Screening of Locally Isolated Actinomycetes and Endophytic Fungi for Production of Bioactive Compounds

Isolation of actinomycetes were done using Humic acid B-vitamins Agar (HVA) while endophytic fungi were from Potato Dextrose Agar (PDA). Isolated strains were then subjected to enzymatic and also antimicrobial testing. Positive strains for antimicrobial testing were then subjected to carbon source u...

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Main Author:	Lim, Jeffrey Seng Heng
Format:	Thesis
Language:	English English
Published:	2006
Subjects:	Fungi - Variation
Online Access:	http://psasir.upm.edu.my/id/eprint/137/1/549086_t_fst_2006_8.pdf
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id	my-upm-ir.137
record_format	uketd_dc
spelling	my-upm-ir.1372013-05-27T06:45:52Z Screening of Locally Isolated Actinomycetes and Endophytic Fungi for Production of Bioactive Compounds 2006-04 Lim, Jeffrey Seng Heng Isolation of actinomycetes were done using Humic acid B-vitamins Agar (HVA) while endophytic fungi were from Potato Dextrose Agar (PDA). Isolated strains were then subjected to enzymatic and also antimicrobial testing. Positive strains for antimicrobial testing were then subjected to carbon source utilization testing and viewed under microscope to determine their spores morphology. From the enzymatic test conducted for actinomycetes, 110 isolates showed positive result for cellulase activity, 107 for xylanase activity and 22 for mannanase activity. Fifteen isolates of endophytic fungi have the ability to degrade cellulose, 28 of the isolates were able to degrade xylan and 12 isolates have the potential to degrade mannan. Thirteen isolates of actinomycetes showed positive result towards the 5 strains of pathogenic microorganisms with the highest on Yersinia enterocolitica. While test done using endophytic fungi showed only 1 isolate with antimicrobial property toward Xanthomonas campestris. Biolog test was done to determine the metabolite diversity of each actinomycetes. Twelve of the 13 isolates of actinomycetes were identified to be from the genus of Streptomyces by observing their spore arrangement and 1 of the isolate could not be identified. Biolog test could not be done on the endophytes fungi strain 13 because this strain does not produce spores.Through microscopic imaging the identity of the endophytes fungi isolate also could not be determined. Actinomycetes isolate number 200 was further identified by targeting its 16S rRNA gene. By using this technique Actinomycetes isolate number 200 was confirmed to be from the genus Streptomyces. Fungi - Variation 2006-04 Thesis http://psasir.upm.edu.my/id/eprint/137/ http://psasir.upm.edu.my/id/eprint/137/1/549086_t_fst_2006_8.pdf application/pdf en public masters Universiti Putra Malaysia Fungi - Variation Faculty of Food Science and Technology English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic	Fungi - Variation
spellingShingle	Fungi - Variation Lim, Jeffrey Seng Heng Screening of Locally Isolated Actinomycetes and Endophytic Fungi for Production of Bioactive Compounds
description	Isolation of actinomycetes were done using Humic acid B-vitamins Agar (HVA) while endophytic fungi were from Potato Dextrose Agar (PDA). Isolated strains were then subjected to enzymatic and also antimicrobial testing. Positive strains for antimicrobial testing were then subjected to carbon source utilization testing and viewed under microscope to determine their spores morphology. From the enzymatic test conducted for actinomycetes, 110 isolates showed positive result for cellulase activity, 107 for xylanase activity and 22 for mannanase activity. Fifteen isolates of endophytic fungi have the ability to degrade cellulose, 28 of the isolates were able to degrade xylan and 12 isolates have the potential to degrade mannan. Thirteen isolates of actinomycetes showed positive result towards the 5 strains of pathogenic microorganisms with the highest on Yersinia enterocolitica. While test done using endophytic fungi showed only 1 isolate with antimicrobial property toward Xanthomonas campestris. Biolog test was done to determine the metabolite diversity of each actinomycetes. Twelve of the 13 isolates of actinomycetes were identified to be from the genus of Streptomyces by observing their spore arrangement and 1 of the isolate could not be identified. Biolog test could not be done on the endophytes fungi strain 13 because this strain does not produce spores.Through microscopic imaging the identity of the endophytes fungi isolate also could not be determined. Actinomycetes isolate number 200 was further identified by targeting its 16S rRNA gene. By using this technique Actinomycetes isolate number 200 was confirmed to be from the genus Streptomyces.
format	Thesis
qualification_level	Master's degree
author	Lim, Jeffrey Seng Heng
author_facet	Lim, Jeffrey Seng Heng
author_sort	Lim, Jeffrey Seng Heng
title	Screening of Locally Isolated Actinomycetes and Endophytic Fungi for Production of Bioactive Compounds
title_short	Screening of Locally Isolated Actinomycetes and Endophytic Fungi for Production of Bioactive Compounds
title_full	Screening of Locally Isolated Actinomycetes and Endophytic Fungi for Production of Bioactive Compounds
title_fullStr	Screening of Locally Isolated Actinomycetes and Endophytic Fungi for Production of Bioactive Compounds
title_full_unstemmed	Screening of Locally Isolated Actinomycetes and Endophytic Fungi for Production of Bioactive Compounds
title_sort	screening of locally isolated actinomycetes and endophytic fungi for production of bioactive compounds
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Food Science and Technology
publishDate	2006
url	http://psasir.upm.edu.my/id/eprint/137/1/549086_t_fst_2006_8.pdf
_version_	1747810144689848320

Screening of Locally Isolated Actinomycetes and Endophytic Fungi for Production of Bioactive Compounds

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