Development, Characterization and Application of Microsatellite Markers of Mangrove Horseshoe Crab, Carcinoscorpius Rotundicauda Latreille in Peninsular Malaysia

Horseshoe crab populations are said to be declining worldwide. However, there is still no published report on the status of horseshoe crabs in Malaysia. In this study, microsatellite markers were developed using 5‟anchored ISSR-PCR enrichment procedure to diagnose population genetic structure of th...

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Main Author:	Abu Bakar, Adibah
Format:	Thesis
Language:	English
Published:	2011
Subjects:	Microsatellites (Genetics) - Peninsular Malaysia Limulus polyphemus - Peninsular Malaysia Crabs - Peninsular Malaysia
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id	my-upm-ir.19441
record_format	uketd_dc
spelling	my-upm-ir.194412014-06-30T07:30:42Z Development, Characterization and Application of Microsatellite Markers of Mangrove Horseshoe Crab, Carcinoscorpius Rotundicauda Latreille in Peninsular Malaysia 2011-07 Abu Bakar, Adibah Horseshoe crab populations are said to be declining worldwide. However, there is still no published report on the status of horseshoe crabs in Malaysia. In this study, microsatellite markers were developed using 5‟anchored ISSR-PCR enrichment procedure to diagnose population genetic structure of the mangrove horseshoe crab, Carcinoscorpius rotundicauda. A total of 134 microsatellite regions have been succesfully isolated using this technique and 36 novel microsatellite markers were developed. Out of these, eleven informative microsatellite loci were identified in an analysis of 127 samples representing five C. rotundicauda populations namely Kuala Juru, Kg. Permatang, Kg. Chuah, Kg. Sekokoh and Kg. Sg. Pulai from Peninsular Malaysia. The mean expected (He) and observed heterozygosity (Ho) values obtained ranged between 0.299-0.421 and 0.148-0.197 respectively. Significant departures from Hardy-Weinberg equilibrium were detected in C. rotundicauda populations at Kg. Permatang, Kg. Chuah and Kg. Sg. Pulai. These populations also show moderate genetic differentiation with overall FST values of 0.108. Pairwise genetic distance values analysed between the five populations studied were close to zero which indicate high genetic similarity between populations. Five polymorphic inter-simple-sequence-repeat (ISSR) markers tested also generated comparable results. Thus, samples in each population are presumed to be genetically similar although the populations studied were geographically distant. Due to the unknown migratory ability of C. rotundicauda species, existing habitats must be conserved for the survival of this species especially in Peninsular Malaysia. Findings from this study provide information on the genetic diversity of C. rotundicauda in Peninsular Malaysia and are hoped to assist in the development of conservation program for the mangrove horseshoe crabs in Southeast Asia. Microsatellites (Genetics) - Peninsular Malaysia Limulus polyphemus - Peninsular Malaysia Crabs - Peninsular Malaysia 2011-07 Thesis http://psasir.upm.edu.my/id/eprint/19441/ masters Universiti Putra Malaysia Microsatellites (Genetics) - Peninsular Malaysia Limulus polyphemus - Peninsular Malaysia Crabs - Peninsular Malaysia Faculty of Biotechnology and Biomolecular Sciences English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
topic	Microsatellites (Genetics) - Peninsular Malaysia Limulus polyphemus - Peninsular Malaysia Crabs - Peninsular Malaysia
spellingShingle	Microsatellites (Genetics) - Peninsular Malaysia Limulus polyphemus - Peninsular Malaysia Crabs - Peninsular Malaysia Abu Bakar, Adibah Development, Characterization and Application of Microsatellite Markers of Mangrove Horseshoe Crab, Carcinoscorpius Rotundicauda Latreille in Peninsular Malaysia
description	Horseshoe crab populations are said to be declining worldwide. However, there is still no published report on the status of horseshoe crabs in Malaysia. In this study, microsatellite markers were developed using 5‟anchored ISSR-PCR enrichment procedure to diagnose population genetic structure of the mangrove horseshoe crab, Carcinoscorpius rotundicauda. A total of 134 microsatellite regions have been succesfully isolated using this technique and 36 novel microsatellite markers were developed. Out of these, eleven informative microsatellite loci were identified in an analysis of 127 samples representing five C. rotundicauda populations namely Kuala Juru, Kg. Permatang, Kg. Chuah, Kg. Sekokoh and Kg. Sg. Pulai from Peninsular Malaysia. The mean expected (He) and observed heterozygosity (Ho) values obtained ranged between 0.299-0.421 and 0.148-0.197 respectively. Significant departures from Hardy-Weinberg equilibrium were detected in C. rotundicauda populations at Kg. Permatang, Kg. Chuah and Kg. Sg. Pulai. These populations also show moderate genetic differentiation with overall FST values of 0.108. Pairwise genetic distance values analysed between the five populations studied were close to zero which indicate high genetic similarity between populations. Five polymorphic inter-simple-sequence-repeat (ISSR) markers tested also generated comparable results. Thus, samples in each population are presumed to be genetically similar although the populations studied were geographically distant. Due to the unknown migratory ability of C. rotundicauda species, existing habitats must be conserved for the survival of this species especially in Peninsular Malaysia. Findings from this study provide information on the genetic diversity of C. rotundicauda in Peninsular Malaysia and are hoped to assist in the development of conservation program for the mangrove horseshoe crabs in Southeast Asia.
format	Thesis
qualification_level	Master's degree
author	Abu Bakar, Adibah
author_facet	Abu Bakar, Adibah
author_sort	Abu Bakar, Adibah
title	Development, Characterization and Application of Microsatellite Markers of Mangrove Horseshoe Crab, Carcinoscorpius Rotundicauda Latreille in Peninsular Malaysia
title_short	Development, Characterization and Application of Microsatellite Markers of Mangrove Horseshoe Crab, Carcinoscorpius Rotundicauda Latreille in Peninsular Malaysia
title_full	Development, Characterization and Application of Microsatellite Markers of Mangrove Horseshoe Crab, Carcinoscorpius Rotundicauda Latreille in Peninsular Malaysia
title_fullStr	Development, Characterization and Application of Microsatellite Markers of Mangrove Horseshoe Crab, Carcinoscorpius Rotundicauda Latreille in Peninsular Malaysia
title_full_unstemmed	Development, Characterization and Application of Microsatellite Markers of Mangrove Horseshoe Crab, Carcinoscorpius Rotundicauda Latreille in Peninsular Malaysia
title_sort	development, characterization and application of microsatellite markers of mangrove horseshoe crab, carcinoscorpius rotundicauda latreille in peninsular malaysia
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Biotechnology and Biomolecular Sciences
publishDate	2011
_version_	1747811382481387520

Development, Characterization and Application of Microsatellite Markers of Mangrove Horseshoe Crab, Carcinoscorpius Rotundicauda Latreille in Peninsular Malaysia

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