Fabrication and Characterization of DNA Sensors from Metal Oxides Nanoparticles and Chitosan-Modified Glassy Carbon Electrodes

Voltammetric studies of a sensitive electrochemical deoxyribonucleic acid (DNA) sensor based on nano particles and multi-walled carbon nanotube (MWCNTs) for DNA immobilization is described. In this study, two nanoparticles were used, zirconium oxide (ZrO2) and titanium oxide (TiO2). Layer deposition...

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Main Author:	Mohd Maamor, Nor Amirah
Format:	Thesis
Language:	English English
Published:	2011
Subjects:	DNA Metallic oxides Nanoparticles
Online Access:	http://psasir.upm.edu.my/id/eprint/19619/1/FS_2011_30.pdf
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id	my-upm-ir.19619
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spelling	my-upm-ir.196192014-04-17T01:46:22Z Fabrication and Characterization of DNA Sensors from Metal Oxides Nanoparticles and Chitosan-Modified Glassy Carbon Electrodes 2011-05 Mohd Maamor, Nor Amirah Voltammetric studies of a sensitive electrochemical deoxyribonucleic acid (DNA) sensor based on nano particles and multi-walled carbon nanotube (MWCNTs) for DNA immobilization is described. In this study, two nanoparticles were used, zirconium oxide (ZrO2) and titanium oxide (TiO2). Layer deposition technique was used to prepare nanoparticles/MWCNTs/chitosan (CHIT)-modified glassy carbon electrode (GCE) and DNA were immobilized to the GCE. The immobilization of DNA on the electrode was monitored by cyclic voltammetry (CV) analysis by measuring the change of peak currents using electroactive methylene blue (MB) as an indicator. Compared with previous DNA sensor with DNA directly incorporated on carbon electrodes, this carbon nanotube-based assay with its large surface area and good charge-transport characteristics increased DNA attachment quantity. Parameters used on this study including electrochemical characterization, scan rate study and pH optimization as well as chronoamperometry (CA) and chronocoulometry (CC). The electrochemical reduction and oxidation of the redox couples of MB (as a DNA indicator) can be recognized easily by the solid-phase voltammetry of nanoparticles. The cyclic voltammograms for the three differently modified electrodes, nanoparticles/CHIT, MWCNTs/CHIT and nanoparticles/MWCNTs/CHIT, showed that there are 2 major peaks due to the redox couple of MB. Electrochemical characterization of the two modified electrodes, nanoparticles/MWCNTs/CHIT, shows that both of this modified electrode give highest peak current, evident that the composite mixtures cause the increase in the redox peak current of MB. For hybridization study, the current enhancement observed in three differently fabricated DNA sensor based on DNA/TiO2/CHIT/GCE, DNA/MWCNTs/CHIT/GCE and DNA/TiO2/MWCNTs/CHIT/GCE, responding to redox couple of MB are compared. The sensor DNA/TiO2/MWCNTs/CHIT/GCE was found to the most be sensitive towards hybridized DNA. Parameters, used in this study include hybridization time, hybridization temperature, accumulation time, effect of varying concentration of probe, calibration curve, selectivity of DNA sensor, reproducibility and repeatability. From the studies, the optimized condition of hybridization for target ssDNA reaching the ssDNA probe is 30 min at 30 oC. The accumulation time of MB, time taken for MB to bind with dsDNA is 150 seconds, while, 100 μM of ssDNA probe was found to be the appropriate dosage for the fabrication of the sensor DNA Metallic oxides Nanoparticles 2011-05 Thesis http://psasir.upm.edu.my/id/eprint/19619/ http://psasir.upm.edu.my/id/eprint/19619/1/FS_2011_30.pdf application/pdf en public masters Universiti Putra Malaysia DNA Metallic oxides Nanoparticles Faculty of Science English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic	DNA Metallic oxides Nanoparticles
spellingShingle	DNA Metallic oxides Nanoparticles Mohd Maamor, Nor Amirah Fabrication and Characterization of DNA Sensors from Metal Oxides Nanoparticles and Chitosan-Modified Glassy Carbon Electrodes
description	Voltammetric studies of a sensitive electrochemical deoxyribonucleic acid (DNA) sensor based on nano particles and multi-walled carbon nanotube (MWCNTs) for DNA immobilization is described. In this study, two nanoparticles were used, zirconium oxide (ZrO2) and titanium oxide (TiO2). Layer deposition technique was used to prepare nanoparticles/MWCNTs/chitosan (CHIT)-modified glassy carbon electrode (GCE) and DNA were immobilized to the GCE. The immobilization of DNA on the electrode was monitored by cyclic voltammetry (CV) analysis by measuring the change of peak currents using electroactive methylene blue (MB) as an indicator. Compared with previous DNA sensor with DNA directly incorporated on carbon electrodes, this carbon nanotube-based assay with its large surface area and good charge-transport characteristics increased DNA attachment quantity. Parameters used on this study including electrochemical characterization, scan rate study and pH optimization as well as chronoamperometry (CA) and chronocoulometry (CC). The electrochemical reduction and oxidation of the redox couples of MB (as a DNA indicator) can be recognized easily by the solid-phase voltammetry of nanoparticles. The cyclic voltammograms for the three differently modified electrodes, nanoparticles/CHIT, MWCNTs/CHIT and nanoparticles/MWCNTs/CHIT, showed that there are 2 major peaks due to the redox couple of MB. Electrochemical characterization of the two modified electrodes, nanoparticles/MWCNTs/CHIT, shows that both of this modified electrode give highest peak current, evident that the composite mixtures cause the increase in the redox peak current of MB. For hybridization study, the current enhancement observed in three differently fabricated DNA sensor based on DNA/TiO2/CHIT/GCE, DNA/MWCNTs/CHIT/GCE and DNA/TiO2/MWCNTs/CHIT/GCE, responding to redox couple of MB are compared. The sensor DNA/TiO2/MWCNTs/CHIT/GCE was found to the most be sensitive towards hybridized DNA. Parameters, used in this study include hybridization time, hybridization temperature, accumulation time, effect of varying concentration of probe, calibration curve, selectivity of DNA sensor, reproducibility and repeatability. From the studies, the optimized condition of hybridization for target ssDNA reaching the ssDNA probe is 30 min at 30 oC. The accumulation time of MB, time taken for MB to bind with dsDNA is 150 seconds, while, 100 μM of ssDNA probe was found to be the appropriate dosage for the fabrication of the sensor
format	Thesis
qualification_level	Master's degree
author	Mohd Maamor, Nor Amirah
author_facet	Mohd Maamor, Nor Amirah
author_sort	Mohd Maamor, Nor Amirah
title	Fabrication and Characterization of DNA Sensors from Metal Oxides Nanoparticles and Chitosan-Modified Glassy Carbon Electrodes
title_short	Fabrication and Characterization of DNA Sensors from Metal Oxides Nanoparticles and Chitosan-Modified Glassy Carbon Electrodes
title_full	Fabrication and Characterization of DNA Sensors from Metal Oxides Nanoparticles and Chitosan-Modified Glassy Carbon Electrodes
title_fullStr	Fabrication and Characterization of DNA Sensors from Metal Oxides Nanoparticles and Chitosan-Modified Glassy Carbon Electrodes
title_full_unstemmed	Fabrication and Characterization of DNA Sensors from Metal Oxides Nanoparticles and Chitosan-Modified Glassy Carbon Electrodes
title_sort	fabrication and characterization of dna sensors from metal oxides nanoparticles and chitosan-modified glassy carbon electrodes
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Science
publishDate	2011
url	http://psasir.upm.edu.my/id/eprint/19619/1/FS_2011_30.pdf
_version_	1747811426086420480

Fabrication and Characterization of DNA Sensors from Metal Oxides Nanoparticles and Chitosan-Modified Glassy Carbon Electrodes

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