Isolation and Characterization of Full-Term Ammoniotic Fluid-derived Stem Cells

Stem cell research has gained many attentions for their hope to cure myriad of disease ranging from genetically linked disorders to neurodegenerative diseases as well as injuries. Pluripotent stem cells signify the perpetual source of versatile cells, making them an excellent source for cell therapy...

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Main Author:	Ferdaos, Nurfarhana
Format:	Thesis
Language:	English English
Published:	2010
Subjects:	Amniotic Fluid Stem Cell
Online Access:	http://psasir.upm.edu.my/id/eprint/21278/1/FPSK%28m%29_2010_20_R.pdf
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id	my-upm-ir.21278
record_format	uketd_dc
spelling	my-upm-ir.212782013-05-27T08:16:03Z Isolation and Characterization of Full-Term Ammoniotic Fluid-derived Stem Cells 2010-10 Ferdaos, Nurfarhana Stem cell research has gained many attentions for their hope to cure myriad of disease ranging from genetically linked disorders to neurodegenerative diseases as well as injuries. Pluripotent stem cells signify the perpetual source of versatile cells, making them an excellent source for cell therapy. Current discoveries have reported amniotic fluid as an alternative source for pluripotent stem cells, termed amniotic fluid as an alternative source for pluripotent stem cells, termed amniotic fluid-derived stem (AFS) cells. AFS cells have been shown to express embryonic and adult stem cell markers while hindering the ethical controversies, which has been the biggest issue involving the well-known pluripotent stem cells; the embryonic stem (ES) cells. In addition, AFS cells also do not form tumors upon transplantation. To date, only amniotic fluid of mid-term pregnancies has been reported to yield AFS cells. However, the collection of mid-term AF samples involves invasive procedure (amniocentesis) that may result in several complications including abortion. Therefore, in study, we aim to isolate and characterize AFS cells from human and rat full-term pregnancies. The heterogeneity of amniotic fluid (AF) cells was observed upon culturing the cells under the optimized conditions, prior to isolation of stem cells by immunoselection against c-Kit, a marker for stem cell factor. The isolation of cells positive for c-Kit using Magnetic-Activated Cell Sorting (MACS) technology has resulted in enrichment of the c-Kit positive cells. Characterization of the c-Kit positive cells has demonstrated the expression of Oct-4, a marker for pluripotent cells and the formation of embryoid bodies (EBs), suggesting the possibility of AFS cells being present during full-term pregnancy in mammals including human. Hence giving hopes that full-term AFS cells would be the future alternative source for stem cell therapy. Amniotic Fluid Stem Cell 2010-10 Thesis http://psasir.upm.edu.my/id/eprint/21278/ http://psasir.upm.edu.my/id/eprint/21278/1/FPSK%28m%29_2010_20_R.pdf application/pdf en public masters Universiti Putra Malaysia Amniotic Fluid Stem Cell Faculty Medicine and Health Sciences English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic	Amniotic Fluid Stem Cell
spellingShingle	Amniotic Fluid Stem Cell Ferdaos, Nurfarhana Isolation and Characterization of Full-Term Ammoniotic Fluid-derived Stem Cells
description	Stem cell research has gained many attentions for their hope to cure myriad of disease ranging from genetically linked disorders to neurodegenerative diseases as well as injuries. Pluripotent stem cells signify the perpetual source of versatile cells, making them an excellent source for cell therapy. Current discoveries have reported amniotic fluid as an alternative source for pluripotent stem cells, termed amniotic fluid as an alternative source for pluripotent stem cells, termed amniotic fluid-derived stem (AFS) cells. AFS cells have been shown to express embryonic and adult stem cell markers while hindering the ethical controversies, which has been the biggest issue involving the well-known pluripotent stem cells; the embryonic stem (ES) cells. In addition, AFS cells also do not form tumors upon transplantation. To date, only amniotic fluid of mid-term pregnancies has been reported to yield AFS cells. However, the collection of mid-term AF samples involves invasive procedure (amniocentesis) that may result in several complications including abortion. Therefore, in study, we aim to isolate and characterize AFS cells from human and rat full-term pregnancies. The heterogeneity of amniotic fluid (AF) cells was observed upon culturing the cells under the optimized conditions, prior to isolation of stem cells by immunoselection against c-Kit, a marker for stem cell factor. The isolation of cells positive for c-Kit using Magnetic-Activated Cell Sorting (MACS) technology has resulted in enrichment of the c-Kit positive cells. Characterization of the c-Kit positive cells has demonstrated the expression of Oct-4, a marker for pluripotent cells and the formation of embryoid bodies (EBs), suggesting the possibility of AFS cells being present during full-term pregnancy in mammals including human. Hence giving hopes that full-term AFS cells would be the future alternative source for stem cell therapy.
format	Thesis
qualification_level	Master's degree
author	Ferdaos, Nurfarhana
author_facet	Ferdaos, Nurfarhana
author_sort	Ferdaos, Nurfarhana
title	Isolation and Characterization of Full-Term Ammoniotic Fluid-derived Stem Cells
title_short	Isolation and Characterization of Full-Term Ammoniotic Fluid-derived Stem Cells
title_full	Isolation and Characterization of Full-Term Ammoniotic Fluid-derived Stem Cells
title_fullStr	Isolation and Characterization of Full-Term Ammoniotic Fluid-derived Stem Cells
title_full_unstemmed	Isolation and Characterization of Full-Term Ammoniotic Fluid-derived Stem Cells
title_sort	isolation and characterization of full-term ammoniotic fluid-derived stem cells
granting_institution	Universiti Putra Malaysia
granting_department	Faculty Medicine and Health Sciences
publishDate	2010
url	http://psasir.upm.edu.my/id/eprint/21278/1/FPSK%28m%29_2010_20_R.pdf
_version_	1747811485712646144

Isolation and Characterization of Full-Term Ammoniotic Fluid-derived Stem Cells

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