Effects of Cola Nut [Cola Nitida (Vent.) Schott & Endl.] Aqueous Extract on Rat Liver During Hepatocarcinogenesis

The effect of Cola nitida aqueous extract in hepatocarcinogenesis induced male Sprague Dawley rat livers, and elemental analysis of the cola nut was studied to investigate the possible anticancer activity. The unprocessed cola nuts were observed for their surface morphological structure under the sc...

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Main Author:	Kadivar, Mohammad
Format:	Thesis
Language:	English English
Published:	2010
Subjects:	Plant Extracts - therapeutic use Cola
Online Access:	http://psasir.upm.edu.my/id/eprint/21416/1/FPSK%28m%29_2010_31_R.pdf
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id	my-upm-ir.21416
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spelling	my-upm-ir.214162013-05-27T08:16:50Z Effects of Cola Nut [Cola Nitida (Vent.) Schott & Endl.] Aqueous Extract on Rat Liver During Hepatocarcinogenesis 2010-11 Kadivar, Mohammad The effect of Cola nitida aqueous extract in hepatocarcinogenesis induced male Sprague Dawley rat livers, and elemental analysis of the cola nut was studied to investigate the possible anticancer activity. The unprocessed cola nuts were observed for their surface morphological structure under the scanning electron microscope (SEM). Together with the imaging, samples were then elementally analyzed using energy dispersive x-ray microanalysis attached to variable pressure scanning electron microscopy (EDX-VPSEM). SEM study of cola nut illustrates numerous crystals packed in clusters within the cell wall. The elemental analysis results revealed that the cola nut contained high amount of oxygen and carbon, in addition to potassium, phosphorus and magnesium. Potassium, magnesium and phosphorous have been well reported as co-factors of antioxidant enzymes to protect the body from oxygen free radicals. Additionally, these elements play important roles in metabolic mechanisms in the body. Hepatocarcinogenesis was induced in rat livers according to the modified Solt and Farber method. Diethylnitrosamine (DEN) was injected into the rats at 200 mg/kg body weight to initiate hepatocarcinogenesis and after two weeks this was followed by feeding 0.02% 2-Acetylaminofluorene (AAF) to promote the hepatocarcinogenesis. The DEN/AAF induced rats were treated with 1, 2.5, and 5% (w/v) concentrations of cola nut extract or 0.001, 0.0025, and 0.005% w/v dilutions of glycyrrhizin as a drug control. There were normal and cancer controls; in addition, three groups of normal rats were treated with three concentrations of cola nut to observe the side effect of the cola nut on normal livers. The body and liver weight profile results of this study showed no significant difference between treated groups compared to normal and cancer controls. The similarity in body weight gain and relative liver weight results might occur because of the short length of the in vivo experiment (eleven weeks). The supplementation of cola nut extract decreased the level of plasma and microsomal GGT and GST tumor marker enzymes significantly in DEN/AAF induced liver tissues even better than glycyrrhizin. Additionally, it was revealed that cola nut extract has no effect on the level of GST and GGT enzymes in normal cells. The histological and ultrastructural examination as well as the lesions scoring results demonstrated that the cola nut extract reduced neoplastic stage of the hepatocarcinogenic liver cells more than glycyrrhizin based on their abnormal morphology, inflammation, necrosis, and fibrosis. Moreover, rat’s normal hepatocytes treated with cola nut extract illustrated normal features. TUNEL assay results showed the significant increase in the number of apoptotic cells in hepatocarcinogenic liver tissues treated with cola nut extract and glycyrrhizin. These results showed that cola nut did not induce the apoptosis in normal liver cells. Quantitative real-time RT-PCR results revealed that although the level of alpha-fetoprotein (AFP) mRNA increased in DEN/AAF induced liver cells, but the supplementation of the cola nut and glycyrrhizin decreased it predominantly in hepatocarcinogenic liver cells treated with cola nut extract. These findings suggest that cola nut might act as a promising anticancer against hepatocarcinogenesis with even higher efficacy compared to glycyrrhizin, without any side effects in normal liver cells. Plant Extracts - therapeutic use Cola 2010-11 Thesis http://psasir.upm.edu.my/id/eprint/21416/ http://psasir.upm.edu.my/id/eprint/21416/1/FPSK%28m%29_2010_31_R.pdf application/pdf en public masters Universiti Putra Malaysia Plant Extracts - therapeutic use Cola Faculty of Medicine and Health Sciences English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic	Plant Extracts - therapeutic use Cola
spellingShingle	Plant Extracts - therapeutic use Cola Kadivar, Mohammad Effects of Cola Nut [Cola Nitida (Vent.) Schott & Endl.] Aqueous Extract on Rat Liver During Hepatocarcinogenesis
description	The effect of Cola nitida aqueous extract in hepatocarcinogenesis induced male Sprague Dawley rat livers, and elemental analysis of the cola nut was studied to investigate the possible anticancer activity. The unprocessed cola nuts were observed for their surface morphological structure under the scanning electron microscope (SEM). Together with the imaging, samples were then elementally analyzed using energy dispersive x-ray microanalysis attached to variable pressure scanning electron microscopy (EDX-VPSEM). SEM study of cola nut illustrates numerous crystals packed in clusters within the cell wall. The elemental analysis results revealed that the cola nut contained high amount of oxygen and carbon, in addition to potassium, phosphorus and magnesium. Potassium, magnesium and phosphorous have been well reported as co-factors of antioxidant enzymes to protect the body from oxygen free radicals. Additionally, these elements play important roles in metabolic mechanisms in the body. Hepatocarcinogenesis was induced in rat livers according to the modified Solt and Farber method. Diethylnitrosamine (DEN) was injected into the rats at 200 mg/kg body weight to initiate hepatocarcinogenesis and after two weeks this was followed by feeding 0.02% 2-Acetylaminofluorene (AAF) to promote the hepatocarcinogenesis. The DEN/AAF induced rats were treated with 1, 2.5, and 5% (w/v) concentrations of cola nut extract or 0.001, 0.0025, and 0.005% w/v dilutions of glycyrrhizin as a drug control. There were normal and cancer controls; in addition, three groups of normal rats were treated with three concentrations of cola nut to observe the side effect of the cola nut on normal livers. The body and liver weight profile results of this study showed no significant difference between treated groups compared to normal and cancer controls. The similarity in body weight gain and relative liver weight results might occur because of the short length of the in vivo experiment (eleven weeks). The supplementation of cola nut extract decreased the level of plasma and microsomal GGT and GST tumor marker enzymes significantly in DEN/AAF induced liver tissues even better than glycyrrhizin. Additionally, it was revealed that cola nut extract has no effect on the level of GST and GGT enzymes in normal cells. The histological and ultrastructural examination as well as the lesions scoring results demonstrated that the cola nut extract reduced neoplastic stage of the hepatocarcinogenic liver cells more than glycyrrhizin based on their abnormal morphology, inflammation, necrosis, and fibrosis. Moreover, rat’s normal hepatocytes treated with cola nut extract illustrated normal features. TUNEL assay results showed the significant increase in the number of apoptotic cells in hepatocarcinogenic liver tissues treated with cola nut extract and glycyrrhizin. These results showed that cola nut did not induce the apoptosis in normal liver cells. Quantitative real-time RT-PCR results revealed that although the level of alpha-fetoprotein (AFP) mRNA increased in DEN/AAF induced liver cells, but the supplementation of the cola nut and glycyrrhizin decreased it predominantly in hepatocarcinogenic liver cells treated with cola nut extract. These findings suggest that cola nut might act as a promising anticancer against hepatocarcinogenesis with even higher efficacy compared to glycyrrhizin, without any side effects in normal liver cells.
format	Thesis
qualification_level	Master's degree
author	Kadivar, Mohammad
author_facet	Kadivar, Mohammad
author_sort	Kadivar, Mohammad
title	Effects of Cola Nut [Cola Nitida (Vent.) Schott & Endl.] Aqueous Extract on Rat Liver During Hepatocarcinogenesis
title_short	Effects of Cola Nut [Cola Nitida (Vent.) Schott & Endl.] Aqueous Extract on Rat Liver During Hepatocarcinogenesis
title_full	Effects of Cola Nut [Cola Nitida (Vent.) Schott & Endl.] Aqueous Extract on Rat Liver During Hepatocarcinogenesis
title_fullStr	Effects of Cola Nut [Cola Nitida (Vent.) Schott & Endl.] Aqueous Extract on Rat Liver During Hepatocarcinogenesis
title_full_unstemmed	Effects of Cola Nut [Cola Nitida (Vent.) Schott & Endl.] Aqueous Extract on Rat Liver During Hepatocarcinogenesis
title_sort	effects of cola nut [cola nitida (vent.) schott & endl.] aqueous extract on rat liver during hepatocarcinogenesis
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Medicine and Health Sciences
publishDate	2010
url	http://psasir.upm.edu.my/id/eprint/21416/1/FPSK%28m%29_2010_31_R.pdf
_version_	1747811488423215104

Effects of Cola Nut [Cola Nitida (Vent.) Schott & Endl.] Aqueous Extract on Rat Liver During Hepatocarcinogenesis

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