Production of Cellulases by Indigenous Fungi (Aspergillus SP. and Trichoderma SP.) for Subsequent Use in Bioconversion of Palm Oil Mill Effluent Solid to Sugar

The isolation of fungi was done from sources such as palm oil plantation, rotten fruits and et cetera, where out of fifty colonies isolated, six strains showed positive result on cellulose degradation and only two cellulolytic fungi were selected to undergo optimization. They were identified as Aspe...

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Main Author:	Wong, Kok Mun
Format:	Thesis
Language:	English
Published:	2005
Subjects:	Cellulase Fungi Aspergillus
Online Access:	http://psasir.upm.edu.my/id/eprint/22010/1/FBSB%202005%2037R.pdf
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id	my-upm-ir.22010
record_format	uketd_dc
spelling	my-upm-ir.220102015-05-22T01:42:04Z Production of Cellulases by Indigenous Fungi (Aspergillus SP. and Trichoderma SP.) for Subsequent Use in Bioconversion of Palm Oil Mill Effluent Solid to Sugar 2005-10 Wong, Kok Mun The isolation of fungi was done from sources such as palm oil plantation, rotten fruits and et cetera, where out of fifty colonies isolated, six strains showed positive result on cellulose degradation and only two cellulolytic fungi were selected to undergo optimization. They were identified as Aspergillus sp and Trichoderma sp respectively. In the optimization process, different parameters were studied in order to increase the cellulases activity for both the fungi. Different types of substrates were tested such as POME solid, carboxylmethylcellulose (CMC) and sugarcane bagasse. From the results obtained, sugarcane bagasse was the best substrate to induce the production of cellulases from the fungi. Therefore, in the optimization process sugarcane bagasse was used to examine the effect of different parameters conditions such as temperature, nitrogen source, inoculum size and inoculum age. The fermentation that was carried out at 37°C, mixture of nitrogen sources consisted of (NH4)2SO4, KNO3, peptone and urea, 10% (v/v) inoculum size and at 48 hours of inoculum age proved to be the best conditions for cellulases production. For Aspergillus sp, 0.85 U/mL FPase, 2.03 U/mL CMCase and 3.00 U/mL β-glucosidase were obtained whereas for Trichoderma sp 0.90 U/mL FPase, 3.00 U/mL CMCase and 0.11 U/mL β-glucosidase were recorded respectively. The crude cellulase obtained was precipitated with ammonium sulphate to be further utilized in the saccharification process. The temperature stability and pH profile of the crude enzyme were also examined. The saccharification process for sugar production was carried out in controlled parameters such as different chemical pretreated POME solids, different ratio of enzyme concentration and different pH. The highest reducing sugars and glucose produced by using the crude enzyme were 22.8 g/L and 14.8 g/L respectively. Comparison on sugar production from POME solid by using diluted commercial cellulase enzyme for sugar production from POME solid was also done. About 0.23 g of reducing sugars and 0.15 g of glucose per gram of substrate was yielded by using the crude enzyme in the saccharification process. In conclusion, the objectives of the study were achieved with the isolation of local cellulase-producing fungi. Cellulase Fungi Aspergillus 2005-10 Thesis http://psasir.upm.edu.my/id/eprint/22010/ http://psasir.upm.edu.my/id/eprint/22010/1/FBSB%202005%2037R.pdf application/pdf en public masters Universiti Putra Malaysia Cellulase Fungi Aspergillus Faculty of Biotechnology and Biomolecular Sciences
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
topic	Cellulase Fungi Aspergillus
spellingShingle	Cellulase Fungi Aspergillus Wong, Kok Mun Production of Cellulases by Indigenous Fungi (Aspergillus SP. and Trichoderma SP.) for Subsequent Use in Bioconversion of Palm Oil Mill Effluent Solid to Sugar
description	The isolation of fungi was done from sources such as palm oil plantation, rotten fruits and et cetera, where out of fifty colonies isolated, six strains showed positive result on cellulose degradation and only two cellulolytic fungi were selected to undergo optimization. They were identified as Aspergillus sp and Trichoderma sp respectively. In the optimization process, different parameters were studied in order to increase the cellulases activity for both the fungi. Different types of substrates were tested such as POME solid, carboxylmethylcellulose (CMC) and sugarcane bagasse. From the results obtained, sugarcane bagasse was the best substrate to induce the production of cellulases from the fungi. Therefore, in the optimization process sugarcane bagasse was used to examine the effect of different parameters conditions such as temperature, nitrogen source, inoculum size and inoculum age. The fermentation that was carried out at 37°C, mixture of nitrogen sources consisted of (NH4)2SO4, KNO3, peptone and urea, 10% (v/v) inoculum size and at 48 hours of inoculum age proved to be the best conditions for cellulases production. For Aspergillus sp, 0.85 U/mL FPase, 2.03 U/mL CMCase and 3.00 U/mL β-glucosidase were obtained whereas for Trichoderma sp 0.90 U/mL FPase, 3.00 U/mL CMCase and 0.11 U/mL β-glucosidase were recorded respectively. The crude cellulase obtained was precipitated with ammonium sulphate to be further utilized in the saccharification process. The temperature stability and pH profile of the crude enzyme were also examined. The saccharification process for sugar production was carried out in controlled parameters such as different chemical pretreated POME solids, different ratio of enzyme concentration and different pH. The highest reducing sugars and glucose produced by using the crude enzyme were 22.8 g/L and 14.8 g/L respectively. Comparison on sugar production from POME solid by using diluted commercial cellulase enzyme for sugar production from POME solid was also done. About 0.23 g of reducing sugars and 0.15 g of glucose per gram of substrate was yielded by using the crude enzyme in the saccharification process. In conclusion, the objectives of the study were achieved with the isolation of local cellulase-producing fungi.
format	Thesis
qualification_level	Master's degree
author	Wong, Kok Mun
author_facet	Wong, Kok Mun
author_sort	Wong, Kok Mun
title	Production of Cellulases by Indigenous Fungi (Aspergillus SP. and Trichoderma SP.) for Subsequent Use in Bioconversion of Palm Oil Mill Effluent Solid to Sugar
title_short	Production of Cellulases by Indigenous Fungi (Aspergillus SP. and Trichoderma SP.) for Subsequent Use in Bioconversion of Palm Oil Mill Effluent Solid to Sugar
title_full	Production of Cellulases by Indigenous Fungi (Aspergillus SP. and Trichoderma SP.) for Subsequent Use in Bioconversion of Palm Oil Mill Effluent Solid to Sugar
title_fullStr	Production of Cellulases by Indigenous Fungi (Aspergillus SP. and Trichoderma SP.) for Subsequent Use in Bioconversion of Palm Oil Mill Effluent Solid to Sugar
title_full_unstemmed	Production of Cellulases by Indigenous Fungi (Aspergillus SP. and Trichoderma SP.) for Subsequent Use in Bioconversion of Palm Oil Mill Effluent Solid to Sugar
title_sort	production of cellulases by indigenous fungi (aspergillus sp. and trichoderma sp.) for subsequent use in bioconversion of palm oil mill effluent solid to sugar
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Biotechnology and Biomolecular Sciences
publishDate	2005
url	http://psasir.upm.edu.my/id/eprint/22010/1/FBSB%202005%2037R.pdf
_version_	1747811510335307776

Production of Cellulases by Indigenous Fungi (Aspergillus SP. and Trichoderma SP.) for Subsequent Use in Bioconversion of Palm Oil Mill Effluent Solid to Sugar

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