Detection, Prevalence and Phylogenetic Analysis of Feline Coronavirus in Malaysia
Feline coronavirus (FCoV) consisted of feline enteric coronavirus (FECV) and feline infectious peritonitis virus (FIPV) which cause a mild disease and fatal disease known as feline infectious peritonitis (FIP), respectively. There is neither effective vaccine, nor curative treatment for FIP, and a f...
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| المؤلف الرئيسي: | |
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| التنسيق: | أطروحة |
| اللغة: | English English |
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2010
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| الوصول للمادة أونلاين: | http://psasir.upm.edu.my/id/eprint/22079/1/FPV%202010%208R.pdf |
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my-upm-ir.220792013-07-08T02:42:33Z Detection, Prevalence and Phylogenetic Analysis of Feline Coronavirus in Malaysia 2010-06 Sharif, Saeed Feline coronavirus (FCoV) consisted of feline enteric coronavirus (FECV) and feline infectious peritonitis virus (FIPV) which cause a mild disease and fatal disease known as feline infectious peritonitis (FIP), respectively. There is neither effective vaccine, nor curative treatment for FIP, and a fast and reliable diagnosis would greatly assist in the management and control of the virus. In this study, a rapid and sensitive approach was established for detection of FCoV using reverse transcriptase polymerase chain reaction (RT-PCR) assay. In the attempt to improve the sensitivity and specificity of the assay, the RT-PCR assay was developed using SYBR Green 1 real-time RT-PCR. By using the RT-PCR assay the prevalence of FCoV was examined in 44 healthy and 28 sick cats suspected of FIP. Results showed FCoV infections in healthy and sick cats were of 84% and 89%, respectively. Phylogenetic analysis was performed on FCoV isolates obtained from 4 healthy and 10 sick cats. The sequence alignment revealed that the local isolates had 96% homology and fall into one main genetic cluster. These findings provided the first genetic information of FCoV in Malaysia. In another study, a duplex RT-PCR assay was developed for detection of mRNS of FCoV in blood samples from 40 healthy and 10 FIP-suspected cats. The results showed that 67.5% of healthy cats were FCoV-positive and the viral mRNA was detected in 15% of animals. In testing of FIP-suspected cats, all cases were positive FCoV with detectable mRNA. Since it is assumed that the viral replication in blood is associated with FIP, the duplex RT-PCR assay is more specific for detection of FIP. However, detecting the viral mRNA by the duplex RT-PCR in asymptomatic cats should be interpreted in conjunction with other clinical findings. Coronavirus infections - Malaysia 2010-06 Thesis http://psasir.upm.edu.my/id/eprint/22079/ http://psasir.upm.edu.my/id/eprint/22079/1/FPV%202010%208R.pdf application/pdf en staffonly masters Universiti Putra Malaysia Coronavirus infections - Malaysia Faculty of Veterinary Medicine English |
| institution |
Universiti Putra Malaysia |
| collection |
PSAS Institutional Repository |
| language |
English English |
| topic |
Coronavirus infections - Malaysia |
| spellingShingle |
Coronavirus infections - Malaysia Sharif, Saeed Detection, Prevalence and Phylogenetic Analysis of Feline Coronavirus in Malaysia |
| description |
Feline coronavirus (FCoV) consisted of feline enteric coronavirus (FECV) and feline infectious peritonitis virus (FIPV) which cause a mild disease and fatal disease known as feline infectious peritonitis (FIP), respectively. There is neither effective vaccine, nor curative treatment for FIP, and a fast and reliable diagnosis would greatly assist in the management and control of the virus. In this study, a rapid and sensitive approach was established for detection of FCoV using reverse transcriptase polymerase chain reaction (RT-PCR) assay. In the attempt to improve the sensitivity and specificity of the assay, the RT-PCR assay was developed using SYBR Green 1 real-time RT-PCR. By using the RT-PCR assay the prevalence of FCoV was examined in 44 healthy and 28 sick cats suspected of FIP. Results showed FCoV infections in healthy and sick cats were of 84% and 89%, respectively. Phylogenetic analysis was performed on FCoV isolates obtained from 4 healthy and 10 sick cats. The sequence alignment revealed that the local isolates had 96% homology and fall into one main genetic cluster. These findings provided the first genetic information of FCoV in Malaysia. In another study, a duplex RT-PCR assay was developed for detection of mRNS of FCoV in blood samples from 40 healthy and 10 FIP-suspected cats. The results showed that 67.5% of healthy cats were FCoV-positive and the viral mRNA was detected in 15% of animals. In testing of FIP-suspected cats, all cases were positive FCoV with detectable mRNA. Since it is assumed that the viral replication in blood is associated with FIP, the duplex RT-PCR assay is more specific for detection of FIP. However, detecting the viral mRNA by the duplex RT-PCR in asymptomatic cats should be interpreted in conjunction with other clinical findings. |
| format |
Thesis |
| qualification_level |
Master's degree |
| author |
Sharif, Saeed |
| author_facet |
Sharif, Saeed |
| author_sort |
Sharif, Saeed |
| title |
Detection, Prevalence and Phylogenetic Analysis of Feline Coronavirus in Malaysia |
| title_short |
Detection, Prevalence and Phylogenetic Analysis of Feline Coronavirus in Malaysia |
| title_full |
Detection, Prevalence and Phylogenetic Analysis of Feline Coronavirus in Malaysia |
| title_fullStr |
Detection, Prevalence and Phylogenetic Analysis of Feline Coronavirus in Malaysia |
| title_full_unstemmed |
Detection, Prevalence and Phylogenetic Analysis of Feline Coronavirus in Malaysia |
| title_sort |
detection, prevalence and phylogenetic analysis of feline coronavirus in malaysia |
| granting_institution |
Universiti Putra Malaysia |
| granting_department |
Faculty of Veterinary Medicine |
| publishDate |
2010 |
| url |
http://psasir.upm.edu.my/id/eprint/22079/1/FPV%202010%208R.pdf |
| _version_ |
1747811515728134144 |