Metarhizium Anisopliae Metch. Sorrokin as a Potential Microbial Control Agent Against the German Cockroach, Blattella Germanica (L.) (Dictyoptera:Blattellidae)

The German cockroach, Blatella germanica (L) is an important urban insect pest in many parts the world. In Malaysia, It is abundant in hotels and food preparative outlets. Several methods are being used to control this pest and the most common method is using chemical insecticides. The use of che...

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Main Author:	Wardani, Raden Isra
Format:	Thesis
Language:	English
Published:	2004
Subjects:	Metarhizium anisopliae - Blatella germanica - Insecticide resistance
Online Access:	http://psasir.upm.edu.my/id/eprint/249/1/549494_FH_2004_11.pdf
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id	my-upm-ir.249
record_format	uketd_dc
spelling	my-upm-ir.2492015-08-06T03:20:09Z Metarhizium Anisopliae Metch. Sorrokin as a Potential Microbial Control Agent Against the German Cockroach, Blattella Germanica (L.) (Dictyoptera:Blattellidae) 2004-11 Wardani, Raden Isra The German cockroach, Blatella germanica (L) is an important urban insect pest in many parts the world. In Malaysia, It is abundant in hotels and food preparative outlets. Several methods are being used to control this pest and the most common method is using chemical insecticides. The use of chemical for controlling cockroaches , however, has incurred a serious drawback. The cockroach population in many parts of the world developed resistant to chemical insecticides. Thus alternative methods for controlling are warranted. One of these methods is the use of biological agents such as entomopathogenic fungus, Metarhizium anisopliae. In this study eleven indigenous isolates of M. anisopliae were evaluated for their pathogenicity to adult male of German cockroach. Monoconidial cultures of the isolates were obtained from soil and insect cadaver that were collected from various localities in Malaysia and were maintained on Potatoes Dextrose Agar+Yeast (PDAY).The result from the screening test shows that from eleven isolates tested, HSAH gave the best result with 87.5% mortality after 13 days inoculation and LT50 values of 6.78 days. Although not significantly different, there was a tendency for mortality on nymphs to be higher than mortality on adult males and females. Further bioassay on four selected isolates, HSAH, Mam, Ckrk, LPR3, shows that the infectivity of the fungus varied with the isolates and dosages to which the cockroaches were exposed. The ED50 values ranged from 2.73 x 109 to 3.15 x 1011 at day 4 after inoculation and 4.19 x 103 to 1.22 x 106 at day 14 after inoculation, the LT50 values ranged from 12.37 to 16.45. The result evidently shows that HSAH isolate was the prominent isolate. Using HSAH isolate to control a cockroach colony, the result shows that highest mortality of 78.5 % was achieved when a colony of 50 insects were used and with dosage of 10:90. The same level of mortality i.e 78.5 % could not be achieved by a colony of 100 insects when applied with a dosage of 10:90. Apparently with a bigger cockroach colony, a higher dosage of fungus was required to kill B. germanica Metarhizium anisopliae - Blatella germanica - Insecticide resistance 2004-11 Thesis http://psasir.upm.edu.my/id/eprint/249/ http://psasir.upm.edu.my/id/eprint/249/1/549494_FH_2004_11.pdf application/pdf en public masters Universiti Putra Malaysia Metarhizium anisopliae - Blatella germanica - Insecticide resistance Faculty of Forestry
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
topic	Metarhizium anisopliae - Blatella germanica - Insecticide resistance
spellingShingle	Metarhizium anisopliae - Blatella germanica - Insecticide resistance Wardani, Raden Isra Metarhizium Anisopliae Metch. Sorrokin as a Potential Microbial Control Agent Against the German Cockroach, Blattella Germanica (L.) (Dictyoptera:Blattellidae)
description	The German cockroach, Blatella germanica (L) is an important urban insect pest in many parts the world. In Malaysia, It is abundant in hotels and food preparative outlets. Several methods are being used to control this pest and the most common method is using chemical insecticides. The use of chemical for controlling cockroaches , however, has incurred a serious drawback. The cockroach population in many parts of the world developed resistant to chemical insecticides. Thus alternative methods for controlling are warranted. One of these methods is the use of biological agents such as entomopathogenic fungus, Metarhizium anisopliae. In this study eleven indigenous isolates of M. anisopliae were evaluated for their pathogenicity to adult male of German cockroach. Monoconidial cultures of the isolates were obtained from soil and insect cadaver that were collected from various localities in Malaysia and were maintained on Potatoes Dextrose Agar+Yeast (PDAY).The result from the screening test shows that from eleven isolates tested, HSAH gave the best result with 87.5% mortality after 13 days inoculation and LT50 values of 6.78 days. Although not significantly different, there was a tendency for mortality on nymphs to be higher than mortality on adult males and females. Further bioassay on four selected isolates, HSAH, Mam, Ckrk, LPR3, shows that the infectivity of the fungus varied with the isolates and dosages to which the cockroaches were exposed. The ED50 values ranged from 2.73 x 109 to 3.15 x 1011 at day 4 after inoculation and 4.19 x 103 to 1.22 x 106 at day 14 after inoculation, the LT50 values ranged from 12.37 to 16.45. The result evidently shows that HSAH isolate was the prominent isolate. Using HSAH isolate to control a cockroach colony, the result shows that highest mortality of 78.5 % was achieved when a colony of 50 insects were used and with dosage of 10:90. The same level of mortality i.e 78.5 % could not be achieved by a colony of 100 insects when applied with a dosage of 10:90. Apparently with a bigger cockroach colony, a higher dosage of fungus was required to kill B. germanica
format	Thesis
qualification_level	Master's degree
author	Wardani, Raden Isra
author_facet	Wardani, Raden Isra
author_sort	Wardani, Raden Isra
title	Metarhizium Anisopliae Metch. Sorrokin as a Potential Microbial Control Agent Against the German Cockroach, Blattella Germanica (L.) (Dictyoptera:Blattellidae)
title_short	Metarhizium Anisopliae Metch. Sorrokin as a Potential Microbial Control Agent Against the German Cockroach, Blattella Germanica (L.) (Dictyoptera:Blattellidae)
title_full	Metarhizium Anisopliae Metch. Sorrokin as a Potential Microbial Control Agent Against the German Cockroach, Blattella Germanica (L.) (Dictyoptera:Blattellidae)
title_fullStr	Metarhizium Anisopliae Metch. Sorrokin as a Potential Microbial Control Agent Against the German Cockroach, Blattella Germanica (L.) (Dictyoptera:Blattellidae)
title_full_unstemmed	Metarhizium Anisopliae Metch. Sorrokin as a Potential Microbial Control Agent Against the German Cockroach, Blattella Germanica (L.) (Dictyoptera:Blattellidae)
title_sort	metarhizium anisopliae metch. sorrokin as a potential microbial control agent against the german cockroach, blattella germanica (l.) (dictyoptera:blattellidae)
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Forestry
publishDate	2004
url	http://psasir.upm.edu.my/id/eprint/249/1/549494_FH_2004_11.pdf
_version_	1747810183991525376

Metarhizium Anisopliae Metch. Sorrokin as a Potential Microbial Control Agent Against the German Cockroach, Blattella Germanica (L.) (Dictyoptera:Blattellidae)

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