Detection and phylogenetic profiling of the RNA2 fragments of noda virus associated with white tail disease in Malaysian Macrobrachium rosenbergii de man by RT-PCR

The detection of nodavirus using RT-PCR and histopathological assay was carried out in Malaysian Macrobrachium rosenbergii. Nodaviruses, which were originally isolated from insects, are small non enveloped riboviruses with two single-stranded RNA genomes, RNA1 and RNA2. The 3.1 kb RNA1 gene encodes...

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Main Author:	Saedi, Tayebeh Azam
Format:	Thesis
Language:	English English
Published:	2011
Subjects:	Macrobrachium rosenbergii - Diseases - Malaysia RNA viruses Shrimps - Phylogeny
Online Access:	http://psasir.upm.edu.my/id/eprint/26564/1/FBSB%202011%2032R.pdf
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id	my-upm-ir.26564
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spelling	my-upm-ir.265642014-01-21T04:06:23Z Detection and phylogenetic profiling of the RNA2 fragments of noda virus associated with white tail disease in Malaysian Macrobrachium rosenbergii de man by RT-PCR 2011-04 Saedi, Tayebeh Azam The detection of nodavirus using RT-PCR and histopathological assay was carried out in Malaysian Macrobrachium rosenbergii. Nodaviruses, which were originally isolated from insects, are small non enveloped riboviruses with two single-stranded RNA genomes, RNA1 and RNA2. The 3.1 kb RNA1 gene encodes the RNAdependent RNA polymerase and the 1.4 kb RNA2 gene encodes the coat protein. The giant fresh water prawn, M. rosenbergii, locally known in Malaysia as ‘Udang Galah’ is widely distributed in most of the tropical and subtropical regions worldwide. The popularity of this prawn has grown rapidly and its demand as food is getting progressively greater. However, wild stocks of M. rosenbergii have seen declines in recent years as they are threatened by over fishing and diseases. Serious outbreaks of diseases in udang galah farms in Malaysia have resulted in high mortality rates. One of the new viral diseases in M. rosenbergii is white tail disease (WTD). Two viruses, M. rosenbergii nodavirus (MrNV) and extra small virus (XSV) have been found to be responsible for this disease. The RT-PCR method was used to detect the 650 bp and the 1114 bp fragments of the RNA2 of MrNV in infected samples. However, the presence of the XSV RNA was detected in two samples of the MrNV- positive samples. Partial nucleotide sequence analysis of the Malaysian isolates showed 98% nucleotide identity with the reported sequence of MrNV isolate from China (AY231437.2). The pathology of the disease was also investigated where progressive myofiber degeneration of the tail muscles, liquifactive myopathy, muscle fiber, fragmentation, granulation, and hemocyte infiltration were observed in the RT-PCRpositive samples for MrNV. Macrobrachium rosenbergii - Diseases - Malaysia RNA viruses Shrimps - Phylogeny 2011-04 Thesis http://psasir.upm.edu.my/id/eprint/26564/ http://psasir.upm.edu.my/id/eprint/26564/1/FBSB%202011%2032R.pdf application/pdf en public masters Universiti Putra Malaysia Macrobrachium rosenbergii - Diseases - Malaysia RNA viruses Shrimps - Phylogeny Faculty of Biotechnology and Biomolecular Sciences English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic	Macrobrachium rosenbergii - Diseases - Malaysia RNA viruses Shrimps - Phylogeny
spellingShingle	Macrobrachium rosenbergii - Diseases - Malaysia RNA viruses Shrimps - Phylogeny Saedi, Tayebeh Azam Detection and phylogenetic profiling of the RNA2 fragments of noda virus associated with white tail disease in Malaysian Macrobrachium rosenbergii de man by RT-PCR
description	The detection of nodavirus using RT-PCR and histopathological assay was carried out in Malaysian Macrobrachium rosenbergii. Nodaviruses, which were originally isolated from insects, are small non enveloped riboviruses with two single-stranded RNA genomes, RNA1 and RNA2. The 3.1 kb RNA1 gene encodes the RNAdependent RNA polymerase and the 1.4 kb RNA2 gene encodes the coat protein. The giant fresh water prawn, M. rosenbergii, locally known in Malaysia as ‘Udang Galah’ is widely distributed in most of the tropical and subtropical regions worldwide. The popularity of this prawn has grown rapidly and its demand as food is getting progressively greater. However, wild stocks of M. rosenbergii have seen declines in recent years as they are threatened by over fishing and diseases. Serious outbreaks of diseases in udang galah farms in Malaysia have resulted in high mortality rates. One of the new viral diseases in M. rosenbergii is white tail disease (WTD). Two viruses, M. rosenbergii nodavirus (MrNV) and extra small virus (XSV) have been found to be responsible for this disease. The RT-PCR method was used to detect the 650 bp and the 1114 bp fragments of the RNA2 of MrNV in infected samples. However, the presence of the XSV RNA was detected in two samples of the MrNV- positive samples. Partial nucleotide sequence analysis of the Malaysian isolates showed 98% nucleotide identity with the reported sequence of MrNV isolate from China (AY231437.2). The pathology of the disease was also investigated where progressive myofiber degeneration of the tail muscles, liquifactive myopathy, muscle fiber, fragmentation, granulation, and hemocyte infiltration were observed in the RT-PCRpositive samples for MrNV.
format	Thesis
qualification_level	Master's degree
author	Saedi, Tayebeh Azam
author_facet	Saedi, Tayebeh Azam
author_sort	Saedi, Tayebeh Azam
title	Detection and phylogenetic profiling of the RNA2 fragments of noda virus associated with white tail disease in Malaysian Macrobrachium rosenbergii de man by RT-PCR
title_short	Detection and phylogenetic profiling of the RNA2 fragments of noda virus associated with white tail disease in Malaysian Macrobrachium rosenbergii de man by RT-PCR
title_full	Detection and phylogenetic profiling of the RNA2 fragments of noda virus associated with white tail disease in Malaysian Macrobrachium rosenbergii de man by RT-PCR
title_fullStr	Detection and phylogenetic profiling of the RNA2 fragments of noda virus associated with white tail disease in Malaysian Macrobrachium rosenbergii de man by RT-PCR
title_full_unstemmed	Detection and phylogenetic profiling of the RNA2 fragments of noda virus associated with white tail disease in Malaysian Macrobrachium rosenbergii de man by RT-PCR
title_sort	detection and phylogenetic profiling of the rna2 fragments of noda virus associated with white tail disease in malaysian macrobrachium rosenbergii de man by rt-pcr
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Biotechnology and Biomolecular Sciences
publishDate	2011
url	http://psasir.upm.edu.my/id/eprint/26564/1/FBSB%202011%2032R.pdf
_version_	1747811547178074112

Detection and phylogenetic profiling of the RNA2 fragments of noda virus associated with white tail disease in Malaysian Macrobrachium rosenbergii de man by RT-PCR

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