Optimization of medium formulation for folate biosynthesis by Lactobacillus plantarum I-UL4 using response surface methodology

Folate plays an important role in human life as one of the most important components for the synthesis of purine, guanine, adenine, pyrimidine and thymine. In this study, lactic acid bacteria (Lactococcus lactis NZ 9000 and MG 1363, Lactobacillus plantarum I-UL4, and Lactobacillus johnsonii DSM 2055...

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Main Author:	Muhamad Nor, Norfarina
Format:	Thesis
Language:	English English
Published:	2011
Subjects:	Folic acid - Synthesis Lactobacillus plantarum Response surfaces (Statistics)
Online Access:	http://psasir.upm.edu.my/id/eprint/27221/1/FBSB%202011%2033R.pdf
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spelling	my-upm-ir.272212014-01-21T04:04:27Z Optimization of medium formulation for folate biosynthesis by Lactobacillus plantarum I-UL4 using response surface methodology 2011-05 Muhamad Nor, Norfarina Folate plays an important role in human life as one of the most important components for the synthesis of purine, guanine, adenine, pyrimidine and thymine. In this study, lactic acid bacteria (Lactococcus lactis NZ 9000 and MG 1363, Lactobacillus plantarum I-UL4, and Lactobacillus johnsonii DSM 20553) were investigated for the biosynthesis of folate. The total folate concentrations in lactic acid bacteria were determined by microbiological assay as a reliable method to evaluate the folate level and Lactobacillus rhamnosus (Lactobacillus casei, ATCC 7469) was used to analyze total folate and responds to most native folates. From this study, all lactic acid bacteria able to synthesized folate and Lb. plantarum I-UL4 exhibited as the best folate biosynthesis and used throughout investigation on the medium optimization. Five components of medium composition (carbon source, lactose, nitrogen source, meat extract and p-aminobenzoic acid (PABA)) were optimized conventionally and the cultivation process was conducted in shake-flask experiment. From the investigation, lactose, meat extract and PABA at concentration 20 g/L, 15 g/L and 10 μM respectively gave significant effect towards growth of Lb. plantarum I-UL4 and folate biosynthesis. Thus, these three concentrations were subsequently used for further medium optimization study based on response surface methodology (RSM). Through RSM approach, twenty experiments of three factors in response to folate biosynthesis were carried out. Estimated optimize conditions of the factors for the growth of Lb. plantarum I-UL4 and folate biosynthesis as suggested by RSM are as follows: lactose, 20 g/L; meat extract, 16.57 g/L and PABA concentration, 10 μM. The optimized medium composition was then further applied in the cultivation process using 2 L stirred tank bioreactor. Finally, about 61.89 μg/L of folate was synthesized by Lb.plantarum I-UL4 using the bioreactor, which was slightly higher than in shake-flask experiment (59.88 μg/L) using the optimized medium composition. In a conclusion, a better understanding of the relationship among the factors was obtained by RSM, which was used as a statistical tool to improve the folate biosynthesis of Lb. plantarum I-UL4 in small and large scale cultivation. Folic acid - Synthesis Lactobacillus plantarum Response surfaces (Statistics) 2011-05 Thesis http://psasir.upm.edu.my/id/eprint/27221/ http://psasir.upm.edu.my/id/eprint/27221/1/FBSB%202011%2033R.pdf application/pdf en public masters Universiti Putra Malaysia Folic acid - Synthesis Lactobacillus plantarum Response surfaces (Statistics) Faculty of Biotechnology and Biomolecular Sciences English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic	Folic acid - Synthesis Lactobacillus plantarum Response surfaces (Statistics)
spellingShingle	Folic acid - Synthesis Lactobacillus plantarum Response surfaces (Statistics) Muhamad Nor, Norfarina Optimization of medium formulation for folate biosynthesis by Lactobacillus plantarum I-UL4 using response surface methodology
description	Folate plays an important role in human life as one of the most important components for the synthesis of purine, guanine, adenine, pyrimidine and thymine. In this study, lactic acid bacteria (Lactococcus lactis NZ 9000 and MG 1363, Lactobacillus plantarum I-UL4, and Lactobacillus johnsonii DSM 20553) were investigated for the biosynthesis of folate. The total folate concentrations in lactic acid bacteria were determined by microbiological assay as a reliable method to evaluate the folate level and Lactobacillus rhamnosus (Lactobacillus casei, ATCC 7469) was used to analyze total folate and responds to most native folates. From this study, all lactic acid bacteria able to synthesized folate and Lb. plantarum I-UL4 exhibited as the best folate biosynthesis and used throughout investigation on the medium optimization. Five components of medium composition (carbon source, lactose, nitrogen source, meat extract and p-aminobenzoic acid (PABA)) were optimized conventionally and the cultivation process was conducted in shake-flask experiment. From the investigation, lactose, meat extract and PABA at concentration 20 g/L, 15 g/L and 10 μM respectively gave significant effect towards growth of Lb. plantarum I-UL4 and folate biosynthesis. Thus, these three concentrations were subsequently used for further medium optimization study based on response surface methodology (RSM). Through RSM approach, twenty experiments of three factors in response to folate biosynthesis were carried out. Estimated optimize conditions of the factors for the growth of Lb. plantarum I-UL4 and folate biosynthesis as suggested by RSM are as follows: lactose, 20 g/L; meat extract, 16.57 g/L and PABA concentration, 10 μM. The optimized medium composition was then further applied in the cultivation process using 2 L stirred tank bioreactor. Finally, about 61.89 μg/L of folate was synthesized by Lb.plantarum I-UL4 using the bioreactor, which was slightly higher than in shake-flask experiment (59.88 μg/L) using the optimized medium composition. In a conclusion, a better understanding of the relationship among the factors was obtained by RSM, which was used as a statistical tool to improve the folate biosynthesis of Lb. plantarum I-UL4 in small and large scale cultivation.
format	Thesis
qualification_level	Master's degree
author	Muhamad Nor, Norfarina
author_facet	Muhamad Nor, Norfarina
author_sort	Muhamad Nor, Norfarina
title	Optimization of medium formulation for folate biosynthesis by Lactobacillus plantarum I-UL4 using response surface methodology
title_short	Optimization of medium formulation for folate biosynthesis by Lactobacillus plantarum I-UL4 using response surface methodology
title_full	Optimization of medium formulation for folate biosynthesis by Lactobacillus plantarum I-UL4 using response surface methodology
title_fullStr	Optimization of medium formulation for folate biosynthesis by Lactobacillus plantarum I-UL4 using response surface methodology
title_full_unstemmed	Optimization of medium formulation for folate biosynthesis by Lactobacillus plantarum I-UL4 using response surface methodology
title_sort	optimization of medium formulation for folate biosynthesis by lactobacillus plantarum i-ul4 using response surface methodology
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Biotechnology and Biomolecular Sciences
publishDate	2011
url	http://psasir.upm.edu.my/id/eprint/27221/1/FBSB%202011%2033R.pdf
_version_	1747811576988041216

Optimization of medium formulation for folate biosynthesis by Lactobacillus plantarum I-UL4 using response surface methodology

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