Biodegradation of petroleum sludge by Methylobacterium sp

A bacterium was isolated from sludge contaminated soil in petroleum refinery centre and tested for its ability to degrade hydrocarbon in petroleum sludge obtained from Shell Refinery Centre, Port Dickson, Negeri Sembilan. The isolate was tentatively identified as Methylobacterium sp. strain ZASH bas...

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Main Author:	Shamsul Harumain, Zakuan Azizi
Format:	Thesis
Language:	English
Published:	2012
Subjects:	Petroleum - Biodegradation Methylobacterium Wood waste
Online Access:	http://psasir.upm.edu.my/id/eprint/31580/1/FBSB%202012%204R.pdf
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id	my-upm-ir.31580
record_format	uketd_dc
spelling	my-upm-ir.315802015-02-02T07:51:55Z Biodegradation of petroleum sludge by Methylobacterium sp 2012-09 Shamsul Harumain, Zakuan Azizi A bacterium was isolated from sludge contaminated soil in petroleum refinery centre and tested for its ability to degrade hydrocarbon in petroleum sludge obtained from Shell Refinery Centre, Port Dickson, Negeri Sembilan. The isolate was tentatively identified as Methylobacterium sp. strain ZASH based on the partial 16s rRNA molecular phylogeny. The optimum condition for the strain to degrade petroleum sludge was characterized and quantified using GCFID. It was revealed that the bacterium degrade optimally between the temperature of 30°C to 35°C, pH 7 to 7.5, 0.5 to 1.5% (v/v) Tween 80 as surfactant source and at 1 to 2% (w/v) peptone as the nitrogen source. Gas chromatography analysis revealed that after 15 days, the Methylobacterium sp. strain ZASH was able to degrade 70% of hydrocarbons component of chain length C12-C36 found in petroleum sludge. It was also found that sawdust can be a good hydrocarbon adsorbent as its addition increase the hydrocarbon removal up to 99% removal. Petroleum - Biodegradation Methylobacterium Wood waste 2012-09 Thesis http://psasir.upm.edu.my/id/eprint/31580/ http://psasir.upm.edu.my/id/eprint/31580/1/FBSB%202012%204R.pdf application/pdf en public masters Universiti Putra Malaysia Petroleum - Biodegradation Methylobacterium Wood waste Faculty of Biotechnology and Biomolecular Sciences
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
topic	Petroleum - Biodegradation Methylobacterium Wood waste
spellingShingle	Petroleum - Biodegradation Methylobacterium Wood waste Shamsul Harumain, Zakuan Azizi Biodegradation of petroleum sludge by Methylobacterium sp
description	A bacterium was isolated from sludge contaminated soil in petroleum refinery centre and tested for its ability to degrade hydrocarbon in petroleum sludge obtained from Shell Refinery Centre, Port Dickson, Negeri Sembilan. The isolate was tentatively identified as Methylobacterium sp. strain ZASH based on the partial 16s rRNA molecular phylogeny. The optimum condition for the strain to degrade petroleum sludge was characterized and quantified using GCFID. It was revealed that the bacterium degrade optimally between the temperature of 30°C to 35°C, pH 7 to 7.5, 0.5 to 1.5% (v/v) Tween 80 as surfactant source and at 1 to 2% (w/v) peptone as the nitrogen source. Gas chromatography analysis revealed that after 15 days, the Methylobacterium sp. strain ZASH was able to degrade 70% of hydrocarbons component of chain length C12-C36 found in petroleum sludge. It was also found that sawdust can be a good hydrocarbon adsorbent as its addition increase the hydrocarbon removal up to 99% removal.
format	Thesis
qualification_level	Master's degree
author	Shamsul Harumain, Zakuan Azizi
author_facet	Shamsul Harumain, Zakuan Azizi
author_sort	Shamsul Harumain, Zakuan Azizi
title	Biodegradation of petroleum sludge by Methylobacterium sp
title_short	Biodegradation of petroleum sludge by Methylobacterium sp
title_full	Biodegradation of petroleum sludge by Methylobacterium sp
title_fullStr	Biodegradation of petroleum sludge by Methylobacterium sp
title_full_unstemmed	Biodegradation of petroleum sludge by Methylobacterium sp
title_sort	biodegradation of petroleum sludge by methylobacterium sp
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Biotechnology and Biomolecular Sciences
publishDate	2012
url	http://psasir.upm.edu.my/id/eprint/31580/1/FBSB%202012%204R.pdf
_version_	1747811616954515456

Biodegradation of petroleum sludge by Methylobacterium sp

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