Electrochemical studies of [Cu(phen)(c-mala)] N03, [Cu(phen)2CI2]·5.5H20, [Cu(phen)(edda)·5.5H20, and their interaction with DNA and H202

Electrochemical characterization of the copper complexes; [Cu(phen)(c-mala)]NO3,[Cu(phen)2Cl2)5]·5.5H2O, and [Cu(phen)(edda)]·5H2O (phen= phenanthroline;c-mala = c-methylanaline; and edda = ethylenediaminediacetic) have been carried out using cyclic voltammetry (CV), chronocoulometry (CC), and chron...

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Main Author:	Yaw, Chong Wey
Format:	Thesis
Language:	English
Published:	2012
Subjects:	Electrochemical analysis Copper DNA
Online Access:	http://psasir.upm.edu.my/id/eprint/31941/1/FS%202012%2040.pdf
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id	my-upm-ir.31941
record_format	uketd_dc
spelling	my-upm-ir.319412015-01-20T01:54:40Z Electrochemical studies of [Cu(phen)(c-mala)] N03, [Cu(phen)2CI2]·5.5H20, [Cu(phen)(edda)·5.5H20, and their interaction with DNA and H202 2012-02 Yaw, Chong Wey Electrochemical characterization of the copper complexes; [Cu(phen)(c-mala)]NO3,[Cu(phen)2Cl2)5]·5.5H2O, and [Cu(phen)(edda)]·5H2O (phen= phenanthroline;c-mala = c-methylanaline; and edda = ethylenediaminediacetic) have been carried out using cyclic voltammetry (CV), chronocoulometry (CC), and chronoamperometry (CA). These copper(II) complexes were found to be electroactive as shown by its well defined redox waveforms during cyclic voltammetry. The results of double potential steps chronocoulometric (CC) studies show the presence of surface charge to be in the order of 10-4 C/cm2. A linear plot of log I versus log scan rate with slope of near 0.5 was obtained indicating the presence of a diffusion controlled process. The monotonic rising current transient observed during chronoamperometric studies provides another evidence of the diffusion controlled process. Based on hydrodynamic voltammetry studies, the diffusion coefficients of [Cu(phen)(c-mala)]NO3; [Cu(phen)2Cl2)5]·5.5H2O and [Cu(phen)(edda)]·5H2O were found to be of the order of 10-7 cm2/s. The interaction between copper(II) complexes and DNA and between copper(II) complexes and H2O2 were also investigated using cyclic voltammetry. The above electrochemical interaction was evident as their CV results revealing a slight shift in peak potential and a significant decrease in redox peak currents of Cu(II) complexes in the presence of DNA. It indicates that the interaction between the copper(II) complex with DNA molecule is via intercalation process. On the other hand, gel electrophoresis showed evident of deformation of DNA by the copper complexes. The “chemical nuclease” activity follows the order: [Cu(phen)2Cl2]•5.5H2O > [Cu(phen)(c-mala)]NO3 > [Cu(phen)edda]•5H2O > CuSO4. Electrochemical analysis Copper DNA 2012-02 Thesis http://psasir.upm.edu.my/id/eprint/31941/ http://psasir.upm.edu.my/id/eprint/31941/1/FS%202012%2040.pdf application/pdf en public masters Universiti Putra Malaysia Electrochemical analysis Copper DNA Faculty of Science
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
topic	Electrochemical analysis Copper DNA
spellingShingle	Electrochemical analysis Copper DNA Yaw, Chong Wey Electrochemical studies of [Cu(phen)(c-mala)] N03, [Cu(phen)2CI2]·5.5H20, [Cu(phen)(edda)·5.5H20, and their interaction with DNA and H202
description	Electrochemical characterization of the copper complexes; [Cu(phen)(c-mala)]NO3,[Cu(phen)2Cl2)5]·5.5H2O, and [Cu(phen)(edda)]·5H2O (phen= phenanthroline;c-mala = c-methylanaline; and edda = ethylenediaminediacetic) have been carried out using cyclic voltammetry (CV), chronocoulometry (CC), and chronoamperometry (CA). These copper(II) complexes were found to be electroactive as shown by its well defined redox waveforms during cyclic voltammetry. The results of double potential steps chronocoulometric (CC) studies show the presence of surface charge to be in the order of 10-4 C/cm2. A linear plot of log I versus log scan rate with slope of near 0.5 was obtained indicating the presence of a diffusion controlled process. The monotonic rising current transient observed during chronoamperometric studies provides another evidence of the diffusion controlled process. Based on hydrodynamic voltammetry studies, the diffusion coefficients of [Cu(phen)(c-mala)]NO3; [Cu(phen)2Cl2)5]·5.5H2O and [Cu(phen)(edda)]·5H2O were found to be of the order of 10-7 cm2/s. The interaction between copper(II) complexes and DNA and between copper(II) complexes and H2O2 were also investigated using cyclic voltammetry. The above electrochemical interaction was evident as their CV results revealing a slight shift in peak potential and a significant decrease in redox peak currents of Cu(II) complexes in the presence of DNA. It indicates that the interaction between the copper(II) complex with DNA molecule is via intercalation process. On the other hand, gel electrophoresis showed evident of deformation of DNA by the copper complexes. The “chemical nuclease” activity follows the order: [Cu(phen)2Cl2]•5.5H2O > [Cu(phen)(c-mala)]NO3 > [Cu(phen)edda]•5H2O > CuSO4.
format	Thesis
qualification_level	Master's degree
author	Yaw, Chong Wey
author_facet	Yaw, Chong Wey
author_sort	Yaw, Chong Wey
title	Electrochemical studies of [Cu(phen)(c-mala)] N03, [Cu(phen)2CI2]·5.5H20, [Cu(phen)(edda)·5.5H20, and their interaction with DNA and H202
title_short	Electrochemical studies of [Cu(phen)(c-mala)] N03, [Cu(phen)2CI2]·5.5H20, [Cu(phen)(edda)·5.5H20, and their interaction with DNA and H202
title_full	Electrochemical studies of [Cu(phen)(c-mala)] N03, [Cu(phen)2CI2]·5.5H20, [Cu(phen)(edda)·5.5H20, and their interaction with DNA and H202
title_fullStr	Electrochemical studies of [Cu(phen)(c-mala)] N03, [Cu(phen)2CI2]·5.5H20, [Cu(phen)(edda)·5.5H20, and their interaction with DNA and H202
title_full_unstemmed	Electrochemical studies of [Cu(phen)(c-mala)] N03, [Cu(phen)2CI2]·5.5H20, [Cu(phen)(edda)·5.5H20, and their interaction with DNA and H202
title_sort	electrochemical studies of [cu(phen)(c-mala)] n03, [cu(phen)2ci2]·5.5h20, [cu(phen)(edda)·5.5h20, and their interaction with dna and h202
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Science
publishDate	2012
url	http://psasir.upm.edu.my/id/eprint/31941/1/FS%202012%2040.pdf
_version_	1747811636830273536

Electrochemical studies of [Cu(phen)(c-mala)] N03, [Cu(phen)2CI2]·5.5H20, [Cu(phen)(edda)·5.5H20, and their interaction with DNA and H202

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