Bacterial flora associated with hatchery-reared juvenile giant tiger prawn, Penaeus monodon (fabricius), and screening of putative bacteria as probiotic candidate

Post larvae of giant tiger prawn, Penaeus monodon, was reared in Puchong hatchery complex of Universiti Putra Malaysia up to juvenile stage. Bacterial flora of different body parts, sediment and rearing water was determined using conventional biochemical methods and GN and GP Biolog microplate syst...

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Main Author:	Zadeh, Shahram Shakiba
Format:	Thesis
Language:	English
Published:	2012
Subjects:	Bacteria Penaeus monodon Probiotics
Online Access:	http://psasir.upm.edu.my/id/eprint/32159/1/FP%202012%2018R.pdf
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id	my-upm-ir.32159
record_format	uketd_dc
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
topic	Bacteria Penaeus monodon Probiotics
spellingShingle	Bacteria Penaeus monodon Probiotics Zadeh, Shahram Shakiba Bacterial flora associated with hatchery-reared juvenile giant tiger prawn, Penaeus monodon (fabricius), and screening of putative bacteria as probiotic candidate
description	Post larvae of giant tiger prawn, Penaeus monodon, was reared in Puchong hatchery complex of Universiti Putra Malaysia up to juvenile stage. Bacterial flora of different body parts, sediment and rearing water was determined using conventional biochemical methods and GN and GP Biolog microplate system. Highest amount of total bacterial count, total Vibrio count and total Pseudomonas count were observed in sediment followed by digestive system (hepatopancrease and intestine). Seven different genera were identified from different body parts, sediment and rearing water comprised of 72% Gram negative bacteria. The predominant bacteria were Vibrio followed by Shewanella, while incident of Shewanella in digestive system was considerably more than sediment, which is indirect evidence for its colonization in digestive system. Thereafter, antagonistic ability of bacterial flora isolates was examined against the most detrimental shrimp pathogens (Vibrio harveyi, Vibrio parahaemolyticus, and Vibrio alginolyticus) using well plate diffusion technique and cross streak technique. The largest inhibitory zones against mentioned shrimp pathogen were produced by Shewanella algae followed by Burkholderia glumae and Bravibacterium linens. Further antagonistic studies were conducted on S. algae in different pH, Salinity, temperature and time using Response Surface methodology. S.algae was able to induce its antagonistic ability against mentioned shrimp pathogens in relatively wide range of experimental conditions. Further observation on growth potential of the candidate probiotic, S. algae, in different pH, salinity, temperature and time was performed using RSM. The candidate probiotic was able to grow and produce anti-Vibrio substances in different environmental condition. Subsequently, the probiotic ability of the candidate probiotic in compare with 3 mentioned pathogens was assessed by means of a ranking index IR. Bacterium with shorter lag period, �, and doubling time, dt, can overcome other bacteria in vivo. Candidate probiotic was faster than V. harveyi and V. algionolyticus but not faster than V. parahaemolyticus. The safety of candidate probiotic for tiger prawn was subsequently examined. Four treatments group of juvenile P. monodon were fed with 2 levels of candidate probiotic supplemented feed (107 and 1010 cfu/g), negative and positive controls for 3 weeks. The highest survival rate was observed in the 2nd treatment (1010 cfu/g) which was significantly different with controls. Low and high salinity stress tests and low temperature stress test were performed within treatment groups to evaluate the effect of candidate probiotic during stressful condition. The 2nd treatment (1010 cfu/g) exhibited significantly higher survival rate within treatments in all stress tests. Consequently, in vivo study of candidate probiotic was assessed through feeding shrimp treatment groups by supplementing commercial shrimp feed with 4 levels of candidate probiotic (T1; 104, T2; 107, T3; 1010 and T4; 1013 cfu/g). The significant highest feed conversion ratio, specific growth rate, protein efficiency ratio and survival was observed in 3rd treatment, this treatment consisted of significantly better bacterial balance and exhibited highest survival rate after 2 weeks challenging with V. harveyi. The possible human risk associated with using of S. algae was derived from lethal dose 50%, LD50, on mammalian model, BALB/C mice. LD50 of candidate probiotic was about 1036 via oral rout of administration which exhibited its safety for mammalian. Generally, S. algae exhibited quite good probiotic characteristics and can be introduced for further commercial and industrial studies.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Zadeh, Shahram Shakiba
author_facet	Zadeh, Shahram Shakiba
author_sort	Zadeh, Shahram Shakiba
title	Bacterial flora associated with hatchery-reared juvenile giant tiger prawn, Penaeus monodon (fabricius), and screening of putative bacteria as probiotic candidate
title_short	Bacterial flora associated with hatchery-reared juvenile giant tiger prawn, Penaeus monodon (fabricius), and screening of putative bacteria as probiotic candidate
title_full	Bacterial flora associated with hatchery-reared juvenile giant tiger prawn, Penaeus monodon (fabricius), and screening of putative bacteria as probiotic candidate
title_fullStr	Bacterial flora associated with hatchery-reared juvenile giant tiger prawn, Penaeus monodon (fabricius), and screening of putative bacteria as probiotic candidate
title_full_unstemmed	Bacterial flora associated with hatchery-reared juvenile giant tiger prawn, Penaeus monodon (fabricius), and screening of putative bacteria as probiotic candidate
title_sort	bacterial flora associated with hatchery-reared juvenile giant tiger prawn, penaeus monodon (fabricius), and screening of putative bacteria as probiotic candidate
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Agriculture
publishDate	2012
url	http://psasir.upm.edu.my/id/eprint/32159/1/FP%202012%2018R.pdf
_version_	1747811647408308224
spelling	my-upm-ir.321592015-01-19T02:55:20Z Bacterial flora associated with hatchery-reared juvenile giant tiger prawn, Penaeus monodon (fabricius), and screening of putative bacteria as probiotic candidate 2012-02 Zadeh, Shahram Shakiba Post larvae of giant tiger prawn, Penaeus monodon, was reared in Puchong hatchery complex of Universiti Putra Malaysia up to juvenile stage. Bacterial flora of different body parts, sediment and rearing water was determined using conventional biochemical methods and GN and GP Biolog microplate system. Highest amount of total bacterial count, total Vibrio count and total Pseudomonas count were observed in sediment followed by digestive system (hepatopancrease and intestine). Seven different genera were identified from different body parts, sediment and rearing water comprised of 72% Gram negative bacteria. The predominant bacteria were Vibrio followed by Shewanella, while incident of Shewanella in digestive system was considerably more than sediment, which is indirect evidence for its colonization in digestive system. Thereafter, antagonistic ability of bacterial flora isolates was examined against the most detrimental shrimp pathogens (Vibrio harveyi, Vibrio parahaemolyticus, and Vibrio alginolyticus) using well plate diffusion technique and cross streak technique. The largest inhibitory zones against mentioned shrimp pathogen were produced by Shewanella algae followed by Burkholderia glumae and Bravibacterium linens. Further antagonistic studies were conducted on S. algae in different pH, Salinity, temperature and time using Response Surface methodology. S.algae was able to induce its antagonistic ability against mentioned shrimp pathogens in relatively wide range of experimental conditions. Further observation on growth potential of the candidate probiotic, S. algae, in different pH, salinity, temperature and time was performed using RSM. The candidate probiotic was able to grow and produce anti-Vibrio substances in different environmental condition. Subsequently, the probiotic ability of the candidate probiotic in compare with 3 mentioned pathogens was assessed by means of a ranking index IR. Bacterium with shorter lag period, �, and doubling time, dt, can overcome other bacteria in vivo. Candidate probiotic was faster than V. harveyi and V. algionolyticus but not faster than V. parahaemolyticus. The safety of candidate probiotic for tiger prawn was subsequently examined. Four treatments group of juvenile P. monodon were fed with 2 levels of candidate probiotic supplemented feed (107 and 1010 cfu/g), negative and positive controls for 3 weeks. The highest survival rate was observed in the 2nd treatment (1010 cfu/g) which was significantly different with controls. Low and high salinity stress tests and low temperature stress test were performed within treatment groups to evaluate the effect of candidate probiotic during stressful condition. The 2nd treatment (1010 cfu/g) exhibited significantly higher survival rate within treatments in all stress tests. Consequently, in vivo study of candidate probiotic was assessed through feeding shrimp treatment groups by supplementing commercial shrimp feed with 4 levels of candidate probiotic (T1; 104, T2; 107, T3; 1010 and T4; 1013 cfu/g). The significant highest feed conversion ratio, specific growth rate, protein efficiency ratio and survival was observed in 3rd treatment, this treatment consisted of significantly better bacterial balance and exhibited highest survival rate after 2 weeks challenging with V. harveyi. The possible human risk associated with using of S. algae was derived from lethal dose 50%, LD50, on mammalian model, BALB/C mice. LD50 of candidate probiotic was about 1036 via oral rout of administration which exhibited its safety for mammalian. Generally, S. algae exhibited quite good probiotic characteristics and can be introduced for further commercial and industrial studies. Bacteria Penaeus monodon Probiotics 2012-02 Thesis http://psasir.upm.edu.my/id/eprint/32159/ http://psasir.upm.edu.my/id/eprint/32159/1/FP%202012%2018R.pdf application/pdf en public phd doctoral Universiti Putra Malaysia Bacteria Penaeus monodon Probiotics Faculty of Agriculture

Bacterial flora associated with hatchery-reared juvenile giant tiger prawn, Penaeus monodon (fabricius), and screening of putative bacteria as probiotic candidate

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