In vitro plant regeneration and RAPD assessment of somaclonal variations in safed musli (Chlorophytum borivilianum Santapau & R.R.Fernandes)

Safed musli (Chlorophytum borivilianum), is a traditional medicinal plant which belongs to liliaceae family and has an increasing demand due to its wide range of uses. It has an aphrodisiac property and form an important ingredient of herbal tonics prescribed in the Ayurvedic systems of medicine. Du...

Full description

Saved in:

Bibliographic Details
Main Author:	Kemat, Nurashikin
Format:	Thesis
Language:	English
Published:	2012
Subjects:	Chlorophytum - Breeding Liliaceae Plant micropropagation
Online Access:	http://psasir.upm.edu.my/id/eprint/32271/1/FP%202012%2032R.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-upm-ir.32271
record_format	uketd_dc
spelling	my-upm-ir.322712015-01-08T02:07:14Z In vitro plant regeneration and RAPD assessment of somaclonal variations in safed musli (Chlorophytum borivilianum Santapau & R.R.Fernandes) 2012-05 Kemat, Nurashikin Safed musli (Chlorophytum borivilianum), is a traditional medicinal plant which belongs to liliaceae family and has an increasing demand due to its wide range of uses. It has an aphrodisiac property and form an important ingredient of herbal tonics prescribed in the Ayurvedic systems of medicine. Due to its medicinal properties and high demand in herbal industry, Safed musli has been introduced in Malaysia by Felda in 2008 under Felda Herbal Corporation Sdn. Bhd. The major constraint in the cultivation of Safed musli is long tuber dormancy at about 6 -7 months, so it’s only one crop per year can be grown. Besides, the other constraints include shortage of quality planting material, lower tuber multiplication rate and impossible to obtain true to type and disease free plants either from seed tuber or seed. A study was carried out using in vitro propagation and molecular characterization of Safed musli. In vitro culture has shown the advantage over the conventional methods of vegetative production as it ensures rapid rate of multiplication and disease free abundant plantlets. Young shoot buds of Safed musli were collected and sterilized. 100% of clean culture and plant survived in sterilization ent with 70% alcohol (6 minutes), 50% Sodium hypochlorite (20 minutes), 0.1% aqueous mercuric chloride (15 minutes). Mass production of shoots was achieved on MS medium supplemented with 3 mg/L BAP which produced the highest mean number of shoots (18.9) and shoot length (6.0) cm. MS medium supplemented with 1 mg/L IBA which produced the highest mean number of roots (52.4) and root length (6.6) cm. In acclimatization, plantlets on media containing vermiculite : organic matter (1:1) showed the highest percentage of survival plants (83%) and mean number of shoot length (18.69) cm. Callus of Safed musli were induced from young shoot bud on MS medium containing 5 mg/L 2,4-D with the highest percentage of callus formation (66.66%) and mean weight of callus (10.65) g. For shoot regeneration, the highest percentage (66.67%) and the highest mean number of shoots per vial (9.6) was obtained on MS medium with 0.5 mg/L BAP . The RAPD assessment of somaclonal variations among Safed musli plantlet was performed. Out of 90 bands, 55 were polymorphic bands and 35 were monomorphic bands ranging between 115.43bp – 3388.24bp. RAPD marker effectively recognized the genetic difference among the in vitro shoot. Chlorophytum - Breeding Liliaceae Plant micropropagation 2012-05 Thesis http://psasir.upm.edu.my/id/eprint/32271/ http://psasir.upm.edu.my/id/eprint/32271/1/FP%202012%2032R.pdf application/pdf en public masters Universiti Putra Malaysia Chlorophytum - Breeding Liliaceae Plant micropropagation
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
topic	Chlorophytum - Breeding Liliaceae Plant micropropagation
spellingShingle	Chlorophytum - Breeding Liliaceae Plant micropropagation Kemat, Nurashikin In vitro plant regeneration and RAPD assessment of somaclonal variations in safed musli (Chlorophytum borivilianum Santapau & R.R.Fernandes)
description	Safed musli (Chlorophytum borivilianum), is a traditional medicinal plant which belongs to liliaceae family and has an increasing demand due to its wide range of uses. It has an aphrodisiac property and form an important ingredient of herbal tonics prescribed in the Ayurvedic systems of medicine. Due to its medicinal properties and high demand in herbal industry, Safed musli has been introduced in Malaysia by Felda in 2008 under Felda Herbal Corporation Sdn. Bhd. The major constraint in the cultivation of Safed musli is long tuber dormancy at about 6 -7 months, so it’s only one crop per year can be grown. Besides, the other constraints include shortage of quality planting material, lower tuber multiplication rate and impossible to obtain true to type and disease free plants either from seed tuber or seed. A study was carried out using in vitro propagation and molecular characterization of Safed musli. In vitro culture has shown the advantage over the conventional methods of vegetative production as it ensures rapid rate of multiplication and disease free abundant plantlets. Young shoot buds of Safed musli were collected and sterilized. 100% of clean culture and plant survived in sterilization ent with 70% alcohol (6 minutes), 50% Sodium hypochlorite (20 minutes), 0.1% aqueous mercuric chloride (15 minutes). Mass production of shoots was achieved on MS medium supplemented with 3 mg/L BAP which produced the highest mean number of shoots (18.9) and shoot length (6.0) cm. MS medium supplemented with 1 mg/L IBA which produced the highest mean number of roots (52.4) and root length (6.6) cm. In acclimatization, plantlets on media containing vermiculite : organic matter (1:1) showed the highest percentage of survival plants (83%) and mean number of shoot length (18.69) cm. Callus of Safed musli were induced from young shoot bud on MS medium containing 5 mg/L 2,4-D with the highest percentage of callus formation (66.66%) and mean weight of callus (10.65) g. For shoot regeneration, the highest percentage (66.67%) and the highest mean number of shoots per vial (9.6) was obtained on MS medium with 0.5 mg/L BAP . The RAPD assessment of somaclonal variations among Safed musli plantlet was performed. Out of 90 bands, 55 were polymorphic bands and 35 were monomorphic bands ranging between 115.43bp – 3388.24bp. RAPD marker effectively recognized the genetic difference among the in vitro shoot.
format	Thesis
qualification_level	Master's degree
author	Kemat, Nurashikin
author_facet	Kemat, Nurashikin
author_sort	Kemat, Nurashikin
title	In vitro plant regeneration and RAPD assessment of somaclonal variations in safed musli (Chlorophytum borivilianum Santapau & R.R.Fernandes)
title_short	In vitro plant regeneration and RAPD assessment of somaclonal variations in safed musli (Chlorophytum borivilianum Santapau & R.R.Fernandes)
title_full	In vitro plant regeneration and RAPD assessment of somaclonal variations in safed musli (Chlorophytum borivilianum Santapau & R.R.Fernandes)
title_fullStr	In vitro plant regeneration and RAPD assessment of somaclonal variations in safed musli (Chlorophytum borivilianum Santapau & R.R.Fernandes)
title_full_unstemmed	In vitro plant regeneration and RAPD assessment of somaclonal variations in safed musli (Chlorophytum borivilianum Santapau & R.R.Fernandes)
title_sort	in vitro plant regeneration and rapd assessment of somaclonal variations in safed musli (chlorophytum borivilianum santapau & r.r.fernandes)
granting_institution	Universiti Putra Malaysia
publishDate	2012
url	http://psasir.upm.edu.my/id/eprint/32271/1/FP%202012%2032R.pdf
_version_	1747811657494560768

In vitro plant regeneration and RAPD assessment of somaclonal variations in safed musli (Chlorophytum borivilianum Santapau & R.R.Fernandes)

Similar Items