Isolation and characterization of hypocholesterolemic bioactive agent extracted from Monascus purpureus FTC5391

One of the basic causes of mortality in developed countries is cardiovascular disease,especially coronary heart disease. Control of blood cholesterol level is important to avoid cardiovascular disease. In this study the bioactive compound(s) of Monascus purpureus FTC5391 with hypocholesterolemic po...

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Main Author:	Ajdari, Zahra
Format:	Thesis
Language:	English
Published:	2012
Subjects:	Monascus purpureus Cardiovascular system - Diseases Blood cholesterol
Online Access:	http://psasir.upm.edu.my/id/eprint/33315/1/FBSB%202012%2022R.pdf
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id	my-upm-ir.33315
record_format	uketd_dc
spelling	my-upm-ir.333152015-03-11T03:59:49Z Isolation and characterization of hypocholesterolemic bioactive agent extracted from Monascus purpureus FTC5391 2012-03 Ajdari, Zahra One of the basic causes of mortality in developed countries is cardiovascular disease,especially coronary heart disease. Control of blood cholesterol level is important to avoid cardiovascular disease. In this study the bioactive compound(s) of Monascus purpureus FTC5391 with hypocholesterolemic potential was investigated. In order to purify and identify the hypocholesterolemic bioactive agent of M. purpureus FTC5391 fermented product, cultivated via submerged fermentation in modified medium was sub-fractionated by solid phase extraction (SPE). The hypocholesterolemic active fraction in rats was selected for further purification by recycling preparative HPLC (RP-HPLC). The effective fraction significantly (P-value < 0.05) regulated the serum lipid-profile of hypercholesterolemic rats as compared with no treated control groups. This fraction increased the serum HDL-C (100%) and decreased the TC (42.6%), LDL-C (46%), TG (54.4%) levels as well as TC/HDL-C ratio (71.9%). Whereas, Atrovastatin, as a positive control, could regulate the rat serum lipid profile by increasing the serum HDL-C (37.5%) and decreasing the serum TC, LDL-C, TG levels and TC/HDL-C ratio 45.15%, 42%, 54.3% and 61.4%, respectively. MS spectrum of the pure effective fraction and its comparison with spectra library suggested the cyclopropane-carboxylic acid 4-dodecanoeil ester as a novel hypocholesterolemic agent. The obvious superiority of this novel compound to gulate the lipid profile in comparison to the atrovastatin and its ability to decrease the liver damage indicator enzymes, revealed the golden perspective of this novel molecule as a potent future hypocholesterolemic drug. Monascus purpureus Cardiovascular system - Diseases Blood cholesterol 2012-03 Thesis http://psasir.upm.edu.my/id/eprint/33315/ http://psasir.upm.edu.my/id/eprint/33315/1/FBSB%202012%2022R.pdf application/pdf en public phd doctoral Universiti Putra Malaysia Monascus purpureus Cardiovascular system - Diseases Blood cholesterol
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
topic	Monascus purpureus Cardiovascular system - Diseases Blood cholesterol
spellingShingle	Monascus purpureus Cardiovascular system - Diseases Blood cholesterol Ajdari, Zahra Isolation and characterization of hypocholesterolemic bioactive agent extracted from Monascus purpureus FTC5391
description	One of the basic causes of mortality in developed countries is cardiovascular disease,especially coronary heart disease. Control of blood cholesterol level is important to avoid cardiovascular disease. In this study the bioactive compound(s) of Monascus purpureus FTC5391 with hypocholesterolemic potential was investigated. In order to purify and identify the hypocholesterolemic bioactive agent of M. purpureus FTC5391 fermented product, cultivated via submerged fermentation in modified medium was sub-fractionated by solid phase extraction (SPE). The hypocholesterolemic active fraction in rats was selected for further purification by recycling preparative HPLC (RP-HPLC). The effective fraction significantly (P-value < 0.05) regulated the serum lipid-profile of hypercholesterolemic rats as compared with no treated control groups. This fraction increased the serum HDL-C (100%) and decreased the TC (42.6%), LDL-C (46%), TG (54.4%) levels as well as TC/HDL-C ratio (71.9%). Whereas, Atrovastatin, as a positive control, could regulate the rat serum lipid profile by increasing the serum HDL-C (37.5%) and decreasing the serum TC, LDL-C, TG levels and TC/HDL-C ratio 45.15%, 42%, 54.3% and 61.4%, respectively. MS spectrum of the pure effective fraction and its comparison with spectra library suggested the cyclopropane-carboxylic acid 4-dodecanoeil ester as a novel hypocholesterolemic agent. The obvious superiority of this novel compound to gulate the lipid profile in comparison to the atrovastatin and its ability to decrease the liver damage indicator enzymes, revealed the golden perspective of this novel molecule as a potent future hypocholesterolemic drug.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Ajdari, Zahra
author_facet	Ajdari, Zahra
author_sort	Ajdari, Zahra
title	Isolation and characterization of hypocholesterolemic bioactive agent extracted from Monascus purpureus FTC5391
title_short	Isolation and characterization of hypocholesterolemic bioactive agent extracted from Monascus purpureus FTC5391
title_full	Isolation and characterization of hypocholesterolemic bioactive agent extracted from Monascus purpureus FTC5391
title_fullStr	Isolation and characterization of hypocholesterolemic bioactive agent extracted from Monascus purpureus FTC5391
title_full_unstemmed	Isolation and characterization of hypocholesterolemic bioactive agent extracted from Monascus purpureus FTC5391
title_sort	isolation and characterization of hypocholesterolemic bioactive agent extracted from monascus purpureus ftc5391
granting_institution	Universiti Putra Malaysia
publishDate	2012
url	http://psasir.upm.edu.my/id/eprint/33315/1/FBSB%202012%2022R.pdf
_version_	1747811692130074624

Isolation and characterization of hypocholesterolemic bioactive agent extracted from Monascus purpureus FTC5391

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