Lc-Ms/Ms Profiling and Characterization of Active Components from Medicinal Gingers (Curcuma Xanthorrhiza and Zingiber Zerumbet)

Ground fresh rhizomes of Zingiber zerumbet and Curcuma xanthorrhiza were exhaustively extracted using acetone, ethanol and water. Acetone extract of Z. zerumbet and ethanol extract of C. xanthorrhiza gave highest yield of crude extract. The crude extracts were fractionated between water and hexane,...

Full description

Saved in:
Bibliographic Details
Main Author: Ruslay, Sharin
Format: Thesis
Language:English
English
Published: 2006
Subjects:
Online Access:http://psasir.upm.edu.my/id/eprint/374/2/549096_ib_2006_6_abstrak_je__dh_pdf_.pdf
Tags: Add Tag
No Tags, Be the first to tag this record!
id my-upm-ir.374
record_format uketd_dc
spelling my-upm-ir.3742013-05-27T06:47:54Z Lc-Ms/Ms Profiling and Characterization of Active Components from Medicinal Gingers (Curcuma Xanthorrhiza and Zingiber Zerumbet) 2006-01 Ruslay, Sharin Ground fresh rhizomes of Zingiber zerumbet and Curcuma xanthorrhiza were exhaustively extracted using acetone, ethanol and water. Acetone extract of Z. zerumbet and ethanol extract of C. xanthorrhiza gave highest yield of crude extract. The crude extracts were fractionated between water and hexane, ethyl acetate and butanol. All the crude extracts and fractions were screened for antioxidant activity. Ethyl acetate and butanol fractions exhibited good antioxidant activity, of which ethyl acetate fractions from both plants showed strongest antioxidant activity. High Performance Liquid Chromatography (HPLC) profiling was done to analyse the peak patterns of extracts and fractions of both plants. Liquid chromatography- UV diode-array and electrospray ionization mass spectroscopy (ESI-MS) have been used to characterize the active fractions of Z. zerumbet and C. xanthorrhiza. The active fraction (ethyl acetate) from Z. zerumbet was analysed to afford kaempferol-3-O-rhamnoside (A) and its isomeric acetyl derivative as kaempferol-3-O-(2” or 3”-O-acetyl)rhamnoside (B), kaempferol-3-O-(4”-O-acetyl)rhamnoside (C), kaempferol-3-O-(3”,4”-O-diacetyl)rhamnoside (D) and kaempferol-3-O-(2”,4”-O-diacetyl)rhamnoside (E). All the structures were confirmed by using various spectroscopic method including HPLC (spiking method), ESI-MS, IR, UV and NMR spectroscopy. Three components, bisdemethoxycurcumin (F), demethoxycurcumin (G) and curcumin (H) were identified from active ethyl acetate fraction of C. xanthorrhiza. The LC-DAD-MS/MS profiling of Z. zerumbet and C. xanthorrhiza have been developed for the first time as per our knowledge. Phytochemical studies on the rhizomes of Z. zerumbet have yielded 7 pure compounds. Hexane fraction afforded zerumbone (1), while ethyl acetate fraction gave demethoxycurcumin (9), kaempferol (11), kaempferol-3-O-rhamnoside (15) or (A), kaempferol-3-O- (4”-O-acetyl)rhamnoside (14) or (C), kaempferol-3-O- (3”, 4”-O-diacetyl)rhamnoside (6) or (D) and kaempferol-3-O- (2”, 4”-O-diacetyl)rhamnoside (18) or (E). Curcuma Medicine, Botanic 2006-01 Thesis http://psasir.upm.edu.my/id/eprint/374/ http://psasir.upm.edu.my/id/eprint/374/2/549096_ib_2006_6_abstrak_je__dh_pdf_.pdf application/pdf en public masters Universiti Putra Malaysia Curcuma Medicine, Botanic Institute of Bioscience English
institution Universiti Putra Malaysia
collection PSAS Institutional Repository
language English
English
topic Curcuma
Curcuma

spellingShingle Curcuma
Curcuma

Ruslay, Sharin
Lc-Ms/Ms Profiling and Characterization of Active Components from Medicinal Gingers (Curcuma Xanthorrhiza and Zingiber Zerumbet)
description Ground fresh rhizomes of Zingiber zerumbet and Curcuma xanthorrhiza were exhaustively extracted using acetone, ethanol and water. Acetone extract of Z. zerumbet and ethanol extract of C. xanthorrhiza gave highest yield of crude extract. The crude extracts were fractionated between water and hexane, ethyl acetate and butanol. All the crude extracts and fractions were screened for antioxidant activity. Ethyl acetate and butanol fractions exhibited good antioxidant activity, of which ethyl acetate fractions from both plants showed strongest antioxidant activity. High Performance Liquid Chromatography (HPLC) profiling was done to analyse the peak patterns of extracts and fractions of both plants. Liquid chromatography- UV diode-array and electrospray ionization mass spectroscopy (ESI-MS) have been used to characterize the active fractions of Z. zerumbet and C. xanthorrhiza. The active fraction (ethyl acetate) from Z. zerumbet was analysed to afford kaempferol-3-O-rhamnoside (A) and its isomeric acetyl derivative as kaempferol-3-O-(2” or 3”-O-acetyl)rhamnoside (B), kaempferol-3-O-(4”-O-acetyl)rhamnoside (C), kaempferol-3-O-(3”,4”-O-diacetyl)rhamnoside (D) and kaempferol-3-O-(2”,4”-O-diacetyl)rhamnoside (E). All the structures were confirmed by using various spectroscopic method including HPLC (spiking method), ESI-MS, IR, UV and NMR spectroscopy. Three components, bisdemethoxycurcumin (F), demethoxycurcumin (G) and curcumin (H) were identified from active ethyl acetate fraction of C. xanthorrhiza. The LC-DAD-MS/MS profiling of Z. zerumbet and C. xanthorrhiza have been developed for the first time as per our knowledge. Phytochemical studies on the rhizomes of Z. zerumbet have yielded 7 pure compounds. Hexane fraction afforded zerumbone (1), while ethyl acetate fraction gave demethoxycurcumin (9), kaempferol (11), kaempferol-3-O-rhamnoside (15) or (A), kaempferol-3-O- (4”-O-acetyl)rhamnoside (14) or (C), kaempferol-3-O- (3”, 4”-O-diacetyl)rhamnoside (6) or (D) and kaempferol-3-O- (2”, 4”-O-diacetyl)rhamnoside (18) or (E).
format Thesis
qualification_level Master's degree
author Ruslay, Sharin
author_facet Ruslay, Sharin
author_sort Ruslay, Sharin
title Lc-Ms/Ms Profiling and Characterization of Active Components from Medicinal Gingers (Curcuma Xanthorrhiza and Zingiber Zerumbet)
title_short Lc-Ms/Ms Profiling and Characterization of Active Components from Medicinal Gingers (Curcuma Xanthorrhiza and Zingiber Zerumbet)
title_full Lc-Ms/Ms Profiling and Characterization of Active Components from Medicinal Gingers (Curcuma Xanthorrhiza and Zingiber Zerumbet)
title_fullStr Lc-Ms/Ms Profiling and Characterization of Active Components from Medicinal Gingers (Curcuma Xanthorrhiza and Zingiber Zerumbet)
title_full_unstemmed Lc-Ms/Ms Profiling and Characterization of Active Components from Medicinal Gingers (Curcuma Xanthorrhiza and Zingiber Zerumbet)
title_sort lc-ms/ms profiling and characterization of active components from medicinal gingers (curcuma xanthorrhiza and zingiber zerumbet)
granting_institution Universiti Putra Malaysia
granting_department Institute of Bioscience
publishDate 2006
url http://psasir.upm.edu.my/id/eprint/374/2/549096_ib_2006_6_abstrak_je__dh_pdf_.pdf
_version_ 1747810206784421888