Analysis of HSP27, APC and β-catenin expressions in gastric cancer, chronic atrophic gastritis and Helicobacter pylori-associated chronic gastritis

Gastric cancer has been noted to cause high mortality since decades ago. It is the seventh most common cancer in Malaysia. It has been said that it is a potentially curable disease if there is an efficient diagnosis of early gastric cancer. Therefore, there have been many studies to identify the bi...

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Main Author:	Tay, Tan Chow
Format:	Thesis
Language:	English
Published:	2013
Subjects:	Biochemical markers Stomach - Cancer Gastritis
Online Access:	http://psasir.upm.edu.my/id/eprint/40018/7/IB%202013%2010R.pdf
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spelling	my-upm-ir.400182016-04-14T07:03:28Z Analysis of HSP27, APC and β-catenin expressions in gastric cancer, chronic atrophic gastritis and Helicobacter pylori-associated chronic gastritis 2013-01 Tay, Tan Chow Gastric cancer has been noted to cause high mortality since decades ago. It is the seventh most common cancer in Malaysia. It has been said that it is a potentially curable disease if there is an efficient diagnosis of early gastric cancer. Therefore, there have been many studies to identify the biomarkers of gastric cancer but unfortunately until today, none has been found to be reliable. This preliminary study was carried out to investigate the role of Hsp27, APC and β-catenin as a possible biomarker of gastric cancer. Besides, the protein expression levels of Hsp27, APC and β-catenin in the precursor lesions were investigate. A total of 48 gastric cancer, 56 chronic atrophic gastritis and 55 Helicobacter pylori-associated chronic gastritis were analyzed by immunohistochemistry. In addition, 30 each from gastric cancer, chronic atrophic gastritis, Helicobacter pylori-associated chronic gastritis and including normal samples were extracted and analyzed with Western blot. Our study demonstrated significant increased of Hsp27 in 97.9% (47/48) gastric cancer, 96.4% (54/56) chronic atrophic gastritis and 96.4% (53/55) Helicobacter pylori-associated chronic gastritis. These expressions were closely correlated with intestinal type gastric cancer (P= 0.001, correlation coefficient= 0.460) as well as moderately and well differentiated gastric cancer (P= 0.024, correlation coefficient= 0.326). For APC, there were significant increased in 83.3% (40/48) gastric cancer, 89.3% (50/56) chronic atrophic gastritis and 83.6% (46/55) H. pylori-associated chronic gastritis. For β-catenin, there were significant increased in 56.3% (27/48) gastric cancer, 25.0% (14/56) chronic atrophic gastritis and 18.2% (10/55) H. pylori-associated chronic gastritis which showed positive immunoreactivity. There was significant correlation between β-catenin expression and age in chronic atrophic gastritis (P= 0.038, correlation coefficient= 0.278). By using Western Blot, the results showed that the Hsp27 expressions were shown to be significant increased in gastric cancer and chronic atrophic gastritis when compared to normal tissues. However, decreased Hsp27 expression was found in H. pylori-associated chronic gastritis. In conclusion, our findings suggests that Hsp27, APC and β-catenin may play a role as possible biomarkers in gastric cancer and precursor lesions since significant increased in protein expression was observed. The results also suggested that deregulated Hsp27, APC and β-catenin occurred as early as in precursor lesions prior to gastric cancer development. Further studies should be performed to further elucidate the role of Hsp27, APC and β-catenin as biomarkers in gastric cancer. Biochemical markers Stomach - Cancer Gastritis 2013-01 Thesis http://psasir.upm.edu.my/id/eprint/40018/ http://psasir.upm.edu.my/id/eprint/40018/7/IB%202013%2010R.pdf application/pdf en public masters Universiti Putra Malaysia Biochemical markers Stomach - Cancer Gastritis
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
topic	Biochemical markers Stomach - Cancer Gastritis
spellingShingle	Biochemical markers Stomach - Cancer Gastritis Tay, Tan Chow Analysis of HSP27, APC and β-catenin expressions in gastric cancer, chronic atrophic gastritis and Helicobacter pylori-associated chronic gastritis
description	Gastric cancer has been noted to cause high mortality since decades ago. It is the seventh most common cancer in Malaysia. It has been said that it is a potentially curable disease if there is an efficient diagnosis of early gastric cancer. Therefore, there have been many studies to identify the biomarkers of gastric cancer but unfortunately until today, none has been found to be reliable. This preliminary study was carried out to investigate the role of Hsp27, APC and β-catenin as a possible biomarker of gastric cancer. Besides, the protein expression levels of Hsp27, APC and β-catenin in the precursor lesions were investigate. A total of 48 gastric cancer, 56 chronic atrophic gastritis and 55 Helicobacter pylori-associated chronic gastritis were analyzed by immunohistochemistry. In addition, 30 each from gastric cancer, chronic atrophic gastritis, Helicobacter pylori-associated chronic gastritis and including normal samples were extracted and analyzed with Western blot. Our study demonstrated significant increased of Hsp27 in 97.9% (47/48) gastric cancer, 96.4% (54/56) chronic atrophic gastritis and 96.4% (53/55) Helicobacter pylori-associated chronic gastritis. These expressions were closely correlated with intestinal type gastric cancer (P= 0.001, correlation coefficient= 0.460) as well as moderately and well differentiated gastric cancer (P= 0.024, correlation coefficient= 0.326). For APC, there were significant increased in 83.3% (40/48) gastric cancer, 89.3% (50/56) chronic atrophic gastritis and 83.6% (46/55) H. pylori-associated chronic gastritis. For β-catenin, there were significant increased in 56.3% (27/48) gastric cancer, 25.0% (14/56) chronic atrophic gastritis and 18.2% (10/55) H. pylori-associated chronic gastritis which showed positive immunoreactivity. There was significant correlation between β-catenin expression and age in chronic atrophic gastritis (P= 0.038, correlation coefficient= 0.278). By using Western Blot, the results showed that the Hsp27 expressions were shown to be significant increased in gastric cancer and chronic atrophic gastritis when compared to normal tissues. However, decreased Hsp27 expression was found in H. pylori-associated chronic gastritis. In conclusion, our findings suggests that Hsp27, APC and β-catenin may play a role as possible biomarkers in gastric cancer and precursor lesions since significant increased in protein expression was observed. The results also suggested that deregulated Hsp27, APC and β-catenin occurred as early as in precursor lesions prior to gastric cancer development. Further studies should be performed to further elucidate the role of Hsp27, APC and β-catenin as biomarkers in gastric cancer.
format	Thesis
qualification_level	Master's degree
author	Tay, Tan Chow
author_facet	Tay, Tan Chow
author_sort	Tay, Tan Chow
title	Analysis of HSP27, APC and β-catenin expressions in gastric cancer, chronic atrophic gastritis and Helicobacter pylori-associated chronic gastritis
title_short	Analysis of HSP27, APC and β-catenin expressions in gastric cancer, chronic atrophic gastritis and Helicobacter pylori-associated chronic gastritis
title_full	Analysis of HSP27, APC and β-catenin expressions in gastric cancer, chronic atrophic gastritis and Helicobacter pylori-associated chronic gastritis
title_fullStr	Analysis of HSP27, APC and β-catenin expressions in gastric cancer, chronic atrophic gastritis and Helicobacter pylori-associated chronic gastritis
title_full_unstemmed	Analysis of HSP27, APC and β-catenin expressions in gastric cancer, chronic atrophic gastritis and Helicobacter pylori-associated chronic gastritis
title_sort	analysis of hsp27, apc and β-catenin expressions in gastric cancer, chronic atrophic gastritis and helicobacter pylori-associated chronic gastritis
granting_institution	Universiti Putra Malaysia
publishDate	2013
url	http://psasir.upm.edu.my/id/eprint/40018/7/IB%202013%2010R.pdf
_version_	1747811835642380288

Analysis of HSP27, APC and β-catenin expressions in gastric cancer, chronic atrophic gastritis and Helicobacter pylori-associated chronic gastritis

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