Biosurfactant Production by Pseudomonas Aeruginosa 181

This study involves the screening of biosurfactant producers that have been isolated from crude oil bacteria degraders. The bacteria were isolated by qualitative screening on cetyltrimethylammonium bromide (CTAB) agar plates and quantitative screening for biosurfactant production in liquid media. A...

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Main Author:	Yassin Al-Araji, Laith Issa
Format:	Thesis
Language:	English English
Published:	2004
Subjects:	Biosurfactants Pseudomonas aeruginosa
Online Access:	http://psasir.upm.edu.my/id/eprint/4812/1/FBSB_2004_2.pdf
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spelling	my-upm-ir.48122013-05-27T07:18:26Z Biosurfactant Production by Pseudomonas Aeruginosa 181 2004 Yassin Al-Araji, Laith Issa This study involves the screening of biosurfactant producers that have been isolated from crude oil bacteria degraders. The bacteria were isolated by qualitative screening on cetyltrimethylammonium bromide (CTAB) agar plates and quantitative screening for biosurfactant production in liquid media. A biosurfactant producer identified as Pseudomonas aeruginosa 181 was selected for further analysis. Maximum biosurfactant production by Pseudomonas aeruginosa 181 was achieved after 120 h incubation at pH 7.0 and 37°C. Static condition and 5.0% bacterial inoculum’s gave the optimum biosurfactant yield. Culture medium containing glucose as the carbon source; and casamino acids as the organic nitrogen source gave the highest level of biosurfactant production. Corn steep liquor and ammonium nitrate on the other hand inhibited biosurfactant production. However, the addition of metal ions such as Fe, Mg and Mn maximized biosurfactant synthesis.The biosurfactant produced by Pseudomonas aeruginosa 181 was purified to homogeneity by acid precipitation and ammonium sulphate precipitation. Biosurfactant produced byPseudomonas aeruginosa 181 was stable and had a broad range of pH from 3.0 to 12.0 with the maximum activity (Surface Tension reduction and Emulsification Index (E24))exhibited at pH 7.0. The purified biosurfactant had a broad range of temperature and exhibited optimum activity at 30°C. This biosurfactant had high activity compared to many commercial surfactants with 0.1 mg critical micelle concentration (CMC). The purified biosurfactant had a maximum emulsification index (E24) of 86% with hexadecane,followed by 80% with nonane, dodecane,tridecane, pentadecane, octadecane and o- Xylene.Response surface methodology (RSM) was then used to study interactive effects of the parameters (pH, stirring rate, casamino acid concentration and incubation period) on the production of biosurfactants. Generally, simultaneously increasing surface tension reduction and emulsification index (E24) improved yields. Production carried out at larger volumes of 1L using Bioreactor under RSM-optimized conditions yielded 350.22 mg of products after purification by acid precipitation. Identities of isolated products were verified by using TLC, high performance liquid chromatography (HPLC), liquid chromatography–Mass spectrometry (LC-MS), mass spectrometry (MS-MS), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and infraredspectroscopy (FT-IR), from analysis carried out the rhamnolipids were monorhamnolipids and dirhamnolipids. Biosurfactants Pseudomonas aeruginosa 2004 Thesis http://psasir.upm.edu.my/id/eprint/4812/ http://psasir.upm.edu.my/id/eprint/4812/1/FBSB_2004_2.pdf application/pdf en public phd doctoral Universiti Putra Malaysia Biosurfactants Pseudomonas aeruginosa Faculty of Biotechnology and Biomolecular Sciences English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic	Biosurfactants Pseudomonas aeruginosa
spellingShingle	Biosurfactants Pseudomonas aeruginosa Yassin Al-Araji, Laith Issa Biosurfactant Production by Pseudomonas Aeruginosa 181
description	This study involves the screening of biosurfactant producers that have been isolated from crude oil bacteria degraders. The bacteria were isolated by qualitative screening on cetyltrimethylammonium bromide (CTAB) agar plates and quantitative screening for biosurfactant production in liquid media. A biosurfactant producer identified as Pseudomonas aeruginosa 181 was selected for further analysis. Maximum biosurfactant production by Pseudomonas aeruginosa 181 was achieved after 120 h incubation at pH 7.0 and 37°C. Static condition and 5.0% bacterial inoculum’s gave the optimum biosurfactant yield. Culture medium containing glucose as the carbon source; and casamino acids as the organic nitrogen source gave the highest level of biosurfactant production. Corn steep liquor and ammonium nitrate on the other hand inhibited biosurfactant production. However, the addition of metal ions such as Fe, Mg and Mn maximized biosurfactant synthesis.The biosurfactant produced by Pseudomonas aeruginosa 181 was purified to homogeneity by acid precipitation and ammonium sulphate precipitation. Biosurfactant produced byPseudomonas aeruginosa 181 was stable and had a broad range of pH from 3.0 to 12.0 with the maximum activity (Surface Tension reduction and Emulsification Index (E24))exhibited at pH 7.0. The purified biosurfactant had a broad range of temperature and exhibited optimum activity at 30°C. This biosurfactant had high activity compared to many commercial surfactants with 0.1 mg critical micelle concentration (CMC). The purified biosurfactant had a maximum emulsification index (E24) of 86% with hexadecane,followed by 80% with nonane, dodecane,tridecane, pentadecane, octadecane and o- Xylene.Response surface methodology (RSM) was then used to study interactive effects of the parameters (pH, stirring rate, casamino acid concentration and incubation period) on the production of biosurfactants. Generally, simultaneously increasing surface tension reduction and emulsification index (E24) improved yields. Production carried out at larger volumes of 1L using Bioreactor under RSM-optimized conditions yielded 350.22 mg of products after purification by acid precipitation. Identities of isolated products were verified by using TLC, high performance liquid chromatography (HPLC), liquid chromatography–Mass spectrometry (LC-MS), mass spectrometry (MS-MS), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and infraredspectroscopy (FT-IR), from analysis carried out the rhamnolipids were monorhamnolipids and dirhamnolipids.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Yassin Al-Araji, Laith Issa
author_facet	Yassin Al-Araji, Laith Issa
author_sort	Yassin Al-Araji, Laith Issa
title	Biosurfactant Production by Pseudomonas Aeruginosa 181
title_short	Biosurfactant Production by Pseudomonas Aeruginosa 181
title_full	Biosurfactant Production by Pseudomonas Aeruginosa 181
title_fullStr	Biosurfactant Production by Pseudomonas Aeruginosa 181
title_full_unstemmed	Biosurfactant Production by Pseudomonas Aeruginosa 181
title_sort	biosurfactant production by pseudomonas aeruginosa 181
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Biotechnology and Biomolecular Sciences
publishDate	2004
url	http://psasir.upm.edu.my/id/eprint/4812/1/FBSB_2004_2.pdf
_version_	1747810289562157056

Biosurfactant Production by Pseudomonas Aeruginosa 181

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