Biological activities of methanolic extract of selected local mushrooms

Local selected mushrooms; Ganoderma boninense, Auricularia auricula judae,Pleurotus cystidiosus and one new unidentified (BS01) were evaluated for the antioxidant, antimicrobial, anti-tyrosinase, anti-hyaluronidase, anti-inflammatory and insulin secretion activities. The antioxidant activity was mea...

Full description

Saved in:

Bibliographic Details
Main Author:	Hashim, Mahfuzatunajla
Format:	Thesis
Language:	English
Published:	2012
Subjects:	Mushrooms Ganoderma Antioxidants
Online Access:	http://psasir.upm.edu.my/id/eprint/48332/7/FBSB%202012%2053R.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-upm-ir.48332
record_format	uketd_dc
spelling	my-upm-ir.483322016-11-04T04:51:08Z Biological activities of methanolic extract of selected local mushrooms 2012-08 Hashim, Mahfuzatunajla Local selected mushrooms; Ganoderma boninense, Auricularia auricula judae,Pleurotus cystidiosus and one new unidentified (BS01) were evaluated for the antioxidant, antimicrobial, anti-tyrosinase, anti-hyaluronidase, anti-inflammatory and insulin secretion activities. The antioxidant activity was measured using the DPPH (1,1-diphenyl-2-picryl hydrazyl) radical scavenging activity assay and ferric reducing antioxidant power assay (FRAP). In antioxidant activity, both G.boninense and A. A. judae showed the highest activity for DPPH and FRAP assays with the lowest IC50 value. The IC50 of G. boninense and A. A. judae for DPPH were 129.8 ± 1.8 and 198.5 ± 1.5 while for FRAP were 25.8 ± 5.0 and 52.7 ± 3.8,respectively. Anti-inflammatory activity was determined by inhibition of nitric oxide (NO) and measuring the nitrite (NO2 -) formation using Griess assay. However, only G. boninense showed inhibitory effect of NO inhibition with IC50 value at 151.3 μg/mL but the extract was also toxic to the RAW 264.7 cell at 500 μg/mL with cell viability percentage of 39.28 ± 2.5% only. Tyrosinase inhibitory was determined by a spectrophotometric method using L-3,4-ihydroxyphenylalanine (L-DOPA) as a substrate. BS01 exhibited significant (p < 0.05) inhibition with the IC50 value at 279.4 μg/mL and G. boninese at 474.4 μg/mL. The colorimetric Morgan-Elson method was carried out for hyaluronidase assay but all of the mushrooms were tested negative. Insulin secretion activity was measured using rat pancreatic β-cell line, BRIN-BD11 cells and the insulin level produced by the cell line was measured by an enzyme-linked immunosorbent assay using a commercial rat insulin ELISA. Among the mushrooms, G. boninense and P.cystidiosus extracts showed significant (p < 0.05) increased in insulin secretion at the concentration of 62.5, 125, 250 and 500 μg/mL. Overall, from the four mushrooms tested, G. boninense seem to exhibit more bioactive compounds and the further work could be done on the isolation, characterization and purification of the active compounds from the crude extract. Mushrooms Ganoderma Antioxidants 2012-08 Thesis http://psasir.upm.edu.my/id/eprint/48332/ http://psasir.upm.edu.my/id/eprint/48332/7/FBSB%202012%2053R.pdf application/pdf en public masters Universiti Putra Malaysia Mushrooms Ganoderma Antioxidants
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
topic	Mushrooms Ganoderma Antioxidants
spellingShingle	Mushrooms Ganoderma Antioxidants Hashim, Mahfuzatunajla Biological activities of methanolic extract of selected local mushrooms
description	Local selected mushrooms; Ganoderma boninense, Auricularia auricula judae,Pleurotus cystidiosus and one new unidentified (BS01) were evaluated for the antioxidant, antimicrobial, anti-tyrosinase, anti-hyaluronidase, anti-inflammatory and insulin secretion activities. The antioxidant activity was measured using the DPPH (1,1-diphenyl-2-picryl hydrazyl) radical scavenging activity assay and ferric reducing antioxidant power assay (FRAP). In antioxidant activity, both G.boninense and A. A. judae showed the highest activity for DPPH and FRAP assays with the lowest IC50 value. The IC50 of G. boninense and A. A. judae for DPPH were 129.8 ± 1.8 and 198.5 ± 1.5 while for FRAP were 25.8 ± 5.0 and 52.7 ± 3.8,respectively. Anti-inflammatory activity was determined by inhibition of nitric oxide (NO) and measuring the nitrite (NO2 -) formation using Griess assay. However, only G. boninense showed inhibitory effect of NO inhibition with IC50 value at 151.3 μg/mL but the extract was also toxic to the RAW 264.7 cell at 500 μg/mL with cell viability percentage of 39.28 ± 2.5% only. Tyrosinase inhibitory was determined by a spectrophotometric method using L-3,4-ihydroxyphenylalanine (L-DOPA) as a substrate. BS01 exhibited significant (p < 0.05) inhibition with the IC50 value at 279.4 μg/mL and G. boninese at 474.4 μg/mL. The colorimetric Morgan-Elson method was carried out for hyaluronidase assay but all of the mushrooms were tested negative. Insulin secretion activity was measured using rat pancreatic β-cell line, BRIN-BD11 cells and the insulin level produced by the cell line was measured by an enzyme-linked immunosorbent assay using a commercial rat insulin ELISA. Among the mushrooms, G. boninense and P.cystidiosus extracts showed significant (p < 0.05) increased in insulin secretion at the concentration of 62.5, 125, 250 and 500 μg/mL. Overall, from the four mushrooms tested, G. boninense seem to exhibit more bioactive compounds and the further work could be done on the isolation, characterization and purification of the active compounds from the crude extract.
format	Thesis
qualification_level	Master's degree
author	Hashim, Mahfuzatunajla
author_facet	Hashim, Mahfuzatunajla
author_sort	Hashim, Mahfuzatunajla
title	Biological activities of methanolic extract of selected local mushrooms
title_short	Biological activities of methanolic extract of selected local mushrooms
title_full	Biological activities of methanolic extract of selected local mushrooms
title_fullStr	Biological activities of methanolic extract of selected local mushrooms
title_full_unstemmed	Biological activities of methanolic extract of selected local mushrooms
title_sort	biological activities of methanolic extract of selected local mushrooms
granting_institution	Universiti Putra Malaysia
publishDate	2012
url	http://psasir.upm.edu.my/id/eprint/48332/7/FBSB%202012%2053R.pdf
_version_	1747811972558094336

Biological activities of methanolic extract of selected local mushrooms

Similar Items