Optimisation of solid-liquid extraction of bioactive compounds from Orthosiphon stamineus benth leaves

Orthosiphon stamineus, also known as Misai Kucing, is one of the invaluable medicinal plants originated from Southeast Asia. O. stamineus leaves were used in plenty of applications related to the medicinal purposes and are believed to cure certain disease such as hypertension, gout and fever. Howeve...

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Main Author:	Abdul Razak, Mohd Farhan
Format:	Thesis
Language:	English
Published:	2012
Subjects:	Biochemical engineering Solid-liquid interfaces
Online Access:	http://psasir.upm.edu.my/id/eprint/48466/1/FK%202012%20113R.pdf
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spelling	my-upm-ir.484662016-09-09T02:19:46Z Optimisation of solid-liquid extraction of bioactive compounds from Orthosiphon stamineus benth leaves 2012-06 Abdul Razak, Mohd Farhan Orthosiphon stamineus, also known as Misai Kucing, is one of the invaluable medicinal plants originated from Southeast Asia. O. stamineus leaves were used in plenty of applications related to the medicinal purposes and are believed to cure certain disease such as hypertension, gout and fever. However, very little research done with regards to ascertain optimum extraction process and quality extract of O. stamineus leaves. This research studied a solid- liquid extraction (SLE) process which are involved in processing of bioactive extract from O. stamineus leaves. Different experiments were carried out to determine the effects of various operating parameters on the qualitative and quantitative aspects of O. stamineus leaves. Rosmarinic acid (RA) and Sinensetin (SEN) were selected as the quality indicators of the product because these two compounds were played a major role in the bioactivities including anti- inflammatory, anti- oxidant and anti- pyretic of O. stamineus leaves. Water is most suitable solvent for extracting O. stamineus leaves compared to ethanol, ethyl acetate, and hexane. This was because water gave the highest extraction yield as well as quality extract that related to RA and SEN. Besides, water also indicated highest antioxidant and anti- inflammatory compared to other solvents. The optimum extraction condition of O. stamineus leaves was determined as 60°C and the ratio of water to solid is 10:1 (ml:g) to avoid degradation of SEN based on analysis using Response Surface Methodology (RSM) technique. of 6 hours were found to be optimum based on analysis of Response Surface Methodology (RSM). Two hours of extraction duration was selected as optimum extraction time based on kinetic study. The activation energy of the extraction is 36.57 kJ mol-1. Biochemical engineering Solid-liquid interfaces 2012-06 Thesis http://psasir.upm.edu.my/id/eprint/48466/ http://psasir.upm.edu.my/id/eprint/48466/1/FK%202012%20113R.pdf application/pdf en public masters Universiti Putra Malaysia Biochemical engineering Solid-liquid interfaces
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
topic	Biochemical engineering Solid-liquid interfaces
spellingShingle	Biochemical engineering Solid-liquid interfaces Abdul Razak, Mohd Farhan Optimisation of solid-liquid extraction of bioactive compounds from Orthosiphon stamineus benth leaves
description	Orthosiphon stamineus, also known as Misai Kucing, is one of the invaluable medicinal plants originated from Southeast Asia. O. stamineus leaves were used in plenty of applications related to the medicinal purposes and are believed to cure certain disease such as hypertension, gout and fever. However, very little research done with regards to ascertain optimum extraction process and quality extract of O. stamineus leaves. This research studied a solid- liquid extraction (SLE) process which are involved in processing of bioactive extract from O. stamineus leaves. Different experiments were carried out to determine the effects of various operating parameters on the qualitative and quantitative aspects of O. stamineus leaves. Rosmarinic acid (RA) and Sinensetin (SEN) were selected as the quality indicators of the product because these two compounds were played a major role in the bioactivities including anti- inflammatory, anti- oxidant and anti- pyretic of O. stamineus leaves. Water is most suitable solvent for extracting O. stamineus leaves compared to ethanol, ethyl acetate, and hexane. This was because water gave the highest extraction yield as well as quality extract that related to RA and SEN. Besides, water also indicated highest antioxidant and anti- inflammatory compared to other solvents. The optimum extraction condition of O. stamineus leaves was determined as 60°C and the ratio of water to solid is 10:1 (ml:g) to avoid degradation of SEN based on analysis using Response Surface Methodology (RSM) technique. of 6 hours were found to be optimum based on analysis of Response Surface Methodology (RSM). Two hours of extraction duration was selected as optimum extraction time based on kinetic study. The activation energy of the extraction is 36.57 kJ mol-1.
format	Thesis
qualification_level	Master's degree
author	Abdul Razak, Mohd Farhan
author_facet	Abdul Razak, Mohd Farhan
author_sort	Abdul Razak, Mohd Farhan
title	Optimisation of solid-liquid extraction of bioactive compounds from Orthosiphon stamineus benth leaves
title_short	Optimisation of solid-liquid extraction of bioactive compounds from Orthosiphon stamineus benth leaves
title_full	Optimisation of solid-liquid extraction of bioactive compounds from Orthosiphon stamineus benth leaves
title_fullStr	Optimisation of solid-liquid extraction of bioactive compounds from Orthosiphon stamineus benth leaves
title_full_unstemmed	Optimisation of solid-liquid extraction of bioactive compounds from Orthosiphon stamineus benth leaves
title_sort	optimisation of solid-liquid extraction of bioactive compounds from orthosiphon stamineus benth leaves
granting_institution	Universiti Putra Malaysia
publishDate	2012
url	http://psasir.upm.edu.my/id/eprint/48466/1/FK%202012%20113R.pdf
_version_	1747811984315777024

Optimisation of solid-liquid extraction of bioactive compounds from Orthosiphon stamineus benth leaves

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