Production Of Laccase By A Locally Isolated Fungus For Biodegradation Of Selected Agrowastes

The present study was conducted to screen the significant laccase (ligninolytic enzyme) producers from locally isolated white-rot fungi using agitated and non-agitated culture conditions. The highest laccase producer, identified as Pycnoporus sp. (strain S16) was selected for the optimisation of lac...

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Main Author:	Ang, Kong Nian
Format:	Thesis
Language:	English English
Published:	2007
Subjects:	Lacasse - Agricultural wastes - Biodegradation
Online Access:	http://psasir.upm.edu.my/id/eprint/4860/1/FBSB_2007_2.pdf
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id	my-upm-ir.4860
record_format	uketd_dc
spelling	my-upm-ir.48602013-05-27T07:18:44Z Production Of Laccase By A Locally Isolated Fungus For Biodegradation Of Selected Agrowastes 2007 Ang, Kong Nian The present study was conducted to screen the significant laccase (ligninolytic enzyme) producers from locally isolated white-rot fungi using agitated and non-agitated culture conditions. The highest laccase producer, identified as Pycnoporus sp. (strain S16) was selected for the optimisation of laccase production with particular attention to the effect of physiological factors on laccase production using submerged culture technique. Laccase was characterised to assist in formulating the best condition for the bioconversion of agro-wastes. Significant laccase production was obtained under carbon-depleted condition and dependent on culture morphology. The culture with high laccase activity was observed with the presence of small mycelial pellets with 1−2 mm diameter. The pellet formation and optimum laccase production influenced by various physiological factors are investigated. The best culture condition obtained consisted of 5 g/L of glucose, 12 mM of nitrogen, inoculum concentration of 8 × 106 spores/mL, initial pH 5, incubation temperature of 37°C and agitation speed of 100 rpm. Under the above culture conditions, the time of fermentation was shortened from 16 days to 7 days with maximum laccase activity of 225 U/L. The characterisation study of concentrated laccase revealed the importance of an acidic condition for the highest laccase activity. The biodegradation study showed that some locally available agro-waste materials are suitable for soluble sugar production and that rice bran gave the best results with 1.90 g/L of soluble sugar produced Lacasse - Agricultural wastes - Biodegradation 2007 Thesis http://psasir.upm.edu.my/id/eprint/4860/ http://psasir.upm.edu.my/id/eprint/4860/1/FBSB_2007_2.pdf application/pdf en public masters Universiti Putra Malaysia Lacasse - Agricultural wastes - Biodegradation Faculty of Biotechnology and Biomolecular sciences English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic	Lacasse - Agricultural wastes - Biodegradation
spellingShingle	Lacasse - Agricultural wastes - Biodegradation Ang, Kong Nian Production Of Laccase By A Locally Isolated Fungus For Biodegradation Of Selected Agrowastes
description	The present study was conducted to screen the significant laccase (ligninolytic enzyme) producers from locally isolated white-rot fungi using agitated and non-agitated culture conditions. The highest laccase producer, identified as Pycnoporus sp. (strain S16) was selected for the optimisation of laccase production with particular attention to the effect of physiological factors on laccase production using submerged culture technique. Laccase was characterised to assist in formulating the best condition for the bioconversion of agro-wastes. Significant laccase production was obtained under carbon-depleted condition and dependent on culture morphology. The culture with high laccase activity was observed with the presence of small mycelial pellets with 1−2 mm diameter. The pellet formation and optimum laccase production influenced by various physiological factors are investigated. The best culture condition obtained consisted of 5 g/L of glucose, 12 mM of nitrogen, inoculum concentration of 8 × 106 spores/mL, initial pH 5, incubation temperature of 37°C and agitation speed of 100 rpm. Under the above culture conditions, the time of fermentation was shortened from 16 days to 7 days with maximum laccase activity of 225 U/L. The characterisation study of concentrated laccase revealed the importance of an acidic condition for the highest laccase activity. The biodegradation study showed that some locally available agro-waste materials are suitable for soluble sugar production and that rice bran gave the best results with 1.90 g/L of soluble sugar produced
format	Thesis
qualification_level	Master's degree
author	Ang, Kong Nian
author_facet	Ang, Kong Nian
author_sort	Ang, Kong Nian
title	Production Of Laccase By A Locally Isolated Fungus For Biodegradation Of Selected Agrowastes
title_short	Production Of Laccase By A Locally Isolated Fungus For Biodegradation Of Selected Agrowastes
title_full	Production Of Laccase By A Locally Isolated Fungus For Biodegradation Of Selected Agrowastes
title_fullStr	Production Of Laccase By A Locally Isolated Fungus For Biodegradation Of Selected Agrowastes
title_full_unstemmed	Production Of Laccase By A Locally Isolated Fungus For Biodegradation Of Selected Agrowastes
title_sort	production of laccase by a locally isolated fungus for biodegradation of selected agrowastes
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Biotechnology and Biomolecular sciences
publishDate	2007
url	http://psasir.upm.edu.my/id/eprint/4860/1/FBSB_2007_2.pdf
_version_	1747810298378584064

Production Of Laccase By A Locally Isolated Fungus For Biodegradation Of Selected Agrowastes

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