Biogas Production and Determination of Methanogens from Digester - Treated Palm Oil Mill Effluent

Due to increasing awareness of the risk of the environmental pollution and emission of green house gases (GHG) that caused global warming, a study of palm oil mill effluent (POME) treatment using biological processes in close digesters has been conducted. Potentially beneficial methane gas productio...

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Main Author:	Zakaria @ Mamat, Mohd Rafein
Format:	Thesis
Language:	English English
Published:	2008
Subjects:	Mathanobacteriaceae - Oil palm - Environmental impact changes
Online Access:	http://psasir.upm.edu.my/id/eprint/4935/1/FBSB_2008_9.pdf
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id	my-upm-ir.4935
record_format	uketd_dc
spelling	my-upm-ir.49352013-05-27T07:19:14Z Biogas Production and Determination of Methanogens from Digester - Treated Palm Oil Mill Effluent 2008 Zakaria @ Mamat, Mohd Rafein Due to increasing awareness of the risk of the environmental pollution and emission of green house gases (GHG) that caused global warming, a study of palm oil mill effluent (POME) treatment using biological processes in close digesters has been conducted. Potentially beneficial methane gas production from this treatment has driven the objectives of this study to explore the methanogens from POME sludge and their characteristic in order to improve the POME treatment efficiency. A pilot plant digester with 500 m3 volume was operated for one year and biogas and methane production profiles were monitored daily. Parameters used for monitoring were pH, temperature, volatile fatty acids, chemical oxygen demand, biogas and methane concentration during the treatment. Structure of the flocs formation and methanogenic bacteria isolated from this digester was also carried out in order to understand the microbiological characteristics of the bioprocess involved Specific methanogenic activity test (SMA) and microscopic observation were carried out to support the existence of the methanogens that were able to produce methane gas. During POME treatment process, the highest methane concentration obtained was 55 % (v/v) and chemical oxygen demand (COD) removal efficiency was up to 95%. Anticipated active biomasses retained in the digester were determined as total solids (TS) and volatile suspended solids (VSS) at 2.5% and 1.5%, respectively. SMA test for acetolastic methanogens was determined in the range of 0.05 - 1.3 g COD/ g VSS/d. Microscopic observation of the sludge and isolated colonies have shown that the digester systems were dominated by microorganism resemble to Methanosaeta sp. and fluorescence microscope has proved that this microorganism exhibited autofluorescence green color. Mathanobacteriaceae - Oil palm - Environmental impact changes 2008 Thesis http://psasir.upm.edu.my/id/eprint/4935/ http://psasir.upm.edu.my/id/eprint/4935/1/FBSB_2008_9.pdf application/pdf en public masters Universiti Putra Malaysia Mathanobacteriaceae - Oil palm - Environmental impact changes Faculty of Biotechnology and Biomolecular Sciences English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic	Mathanobacteriaceae - Oil palm - Environmental impact changes
spellingShingle	Mathanobacteriaceae - Oil palm - Environmental impact changes Zakaria @ Mamat, Mohd Rafein Biogas Production and Determination of Methanogens from Digester - Treated Palm Oil Mill Effluent
description	Due to increasing awareness of the risk of the environmental pollution and emission of green house gases (GHG) that caused global warming, a study of palm oil mill effluent (POME) treatment using biological processes in close digesters has been conducted. Potentially beneficial methane gas production from this treatment has driven the objectives of this study to explore the methanogens from POME sludge and their characteristic in order to improve the POME treatment efficiency. A pilot plant digester with 500 m3 volume was operated for one year and biogas and methane production profiles were monitored daily. Parameters used for monitoring were pH, temperature, volatile fatty acids, chemical oxygen demand, biogas and methane concentration during the treatment. Structure of the flocs formation and methanogenic bacteria isolated from this digester was also carried out in order to understand the microbiological characteristics of the bioprocess involved Specific methanogenic activity test (SMA) and microscopic observation were carried out to support the existence of the methanogens that were able to produce methane gas. During POME treatment process, the highest methane concentration obtained was 55 % (v/v) and chemical oxygen demand (COD) removal efficiency was up to 95%. Anticipated active biomasses retained in the digester were determined as total solids (TS) and volatile suspended solids (VSS) at 2.5% and 1.5%, respectively. SMA test for acetolastic methanogens was determined in the range of 0.05 - 1.3 g COD/ g VSS/d. Microscopic observation of the sludge and isolated colonies have shown that the digester systems were dominated by microorganism resemble to Methanosaeta sp. and fluorescence microscope has proved that this microorganism exhibited autofluorescence green color.
format	Thesis
qualification_level	Master's degree
author	Zakaria @ Mamat, Mohd Rafein
author_facet	Zakaria @ Mamat, Mohd Rafein
author_sort	Zakaria @ Mamat, Mohd Rafein
title	Biogas Production and Determination of Methanogens from Digester - Treated Palm Oil Mill Effluent
title_short	Biogas Production and Determination of Methanogens from Digester - Treated Palm Oil Mill Effluent
title_full	Biogas Production and Determination of Methanogens from Digester - Treated Palm Oil Mill Effluent
title_fullStr	Biogas Production and Determination of Methanogens from Digester - Treated Palm Oil Mill Effluent
title_full_unstemmed	Biogas Production and Determination of Methanogens from Digester - Treated Palm Oil Mill Effluent
title_sort	biogas production and determination of methanogens from digester - treated palm oil mill effluent
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Biotechnology and Biomolecular Sciences
publishDate	2008
url	http://psasir.upm.edu.my/id/eprint/4935/1/FBSB_2008_9.pdf
_version_	1747810313848225792

Biogas Production and Determination of Methanogens from Digester - Treated Palm Oil Mill Effluent

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