Synthesis Of Bio-Inorganic Nanohybrids Material (Binhs) Of Deoxyribonucleic Acid Encapsulated Into Layered Double Hydroxide

Deoxyribonucleic acid (DNA) from salmon sperm was encapsulated into the inorganic Zn/Al-layered double hydroxides (LDHs) interlamellar for the formation of bio-inorganic nanohybrids materials (BINHs) by self-assembly method. Intercalation of DNA into Zn/Al-layered double hydroxides inorganic interla...

Full description

Saved in:

Bibliographic Details
Main Author:	Suyub, Iswan Budy
Format:	Thesis
Language:	English English
Published:	2008
Subjects:	Hydroxides
Online Access:	http://psasir.upm.edu.my/id/eprint/4938/1/FBSB_2008_12a.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-upm-ir.4938
record_format	uketd_dc
spelling	my-upm-ir.49382013-05-27T07:19:16Z Synthesis Of Bio-Inorganic Nanohybrids Material (Binhs) Of Deoxyribonucleic Acid Encapsulated Into Layered Double Hydroxide 2008 Suyub, Iswan Budy Deoxyribonucleic acid (DNA) from salmon sperm was encapsulated into the inorganic Zn/Al-layered double hydroxides (LDHs) interlamellar for the formation of bio-inorganic nanohybrids materials (BINHs) by self-assembly method. Intercalation of DNA into Zn/Al-layered double hydroxides inorganic interlamellar host was carried out at different Zn to Al molar ratios, Rs and various concentrations of DNA. This work is meant to synthesize BINHs as drug or gene carrier. Among the numerous DNA delivery approaches developed so far, viral methods are well known and can be extremely efficient as its were used in the first human gene therapy test, but the safety especially the toxicity and immunogenecity of viral vectors is unknown. The physicochemical properties of the as-synthesized LDH and the resulting BINHs nanocomposite were studied using powder x-ray diffraction (PXRD) analysis. Expansion of basal spacing to about 21.2 Å (for R=3 with highest concentration of DNA which is 100 mg/ml) and 21.9 Å (for R=1 with lowest concentration of DNA which is 44.4 mg/ml) in the resulting nanomaterials was observed compared to their respective LDHs precursor with basal spacing of 9.0 Å and 8.9 Å, respectively. This shows that the expansion is due to spatial orientation of the DNA into the LDH inorganic interlamellar and FTIR spectra further confirmed that the DNA was intercalated into the host-matrix of the LDHs. Polymerase Chain Reaction (PCR) analysis verified that DNA within LDH interlayer still sustained its integrity because amplification of certain region of DNA can still be carried out. Hydroxides 2008 Thesis http://psasir.upm.edu.my/id/eprint/4938/ http://psasir.upm.edu.my/id/eprint/4938/1/FBSB_2008_12a.pdf application/pdf en public masters Universiti Putra Malaysia Hydroxides Faculty of Biotechnology and Biomolecular sciences English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic	Hydroxides
spellingShingle	Hydroxides Suyub, Iswan Budy Synthesis Of Bio-Inorganic Nanohybrids Material (Binhs) Of Deoxyribonucleic Acid Encapsulated Into Layered Double Hydroxide
description	Deoxyribonucleic acid (DNA) from salmon sperm was encapsulated into the inorganic Zn/Al-layered double hydroxides (LDHs) interlamellar for the formation of bio-inorganic nanohybrids materials (BINHs) by self-assembly method. Intercalation of DNA into Zn/Al-layered double hydroxides inorganic interlamellar host was carried out at different Zn to Al molar ratios, Rs and various concentrations of DNA. This work is meant to synthesize BINHs as drug or gene carrier. Among the numerous DNA delivery approaches developed so far, viral methods are well known and can be extremely efficient as its were used in the first human gene therapy test, but the safety especially the toxicity and immunogenecity of viral vectors is unknown. The physicochemical properties of the as-synthesized LDH and the resulting BINHs nanocomposite were studied using powder x-ray diffraction (PXRD) analysis. Expansion of basal spacing to about 21.2 Å (for R=3 with highest concentration of DNA which is 100 mg/ml) and 21.9 Å (for R=1 with lowest concentration of DNA which is 44.4 mg/ml) in the resulting nanomaterials was observed compared to their respective LDHs precursor with basal spacing of 9.0 Å and 8.9 Å, respectively. This shows that the expansion is due to spatial orientation of the DNA into the LDH inorganic interlamellar and FTIR spectra further confirmed that the DNA was intercalated into the host-matrix of the LDHs. Polymerase Chain Reaction (PCR) analysis verified that DNA within LDH interlayer still sustained its integrity because amplification of certain region of DNA can still be carried out.
format	Thesis
qualification_level	Master's degree
author	Suyub, Iswan Budy
author_facet	Suyub, Iswan Budy
author_sort	Suyub, Iswan Budy
title	Synthesis Of Bio-Inorganic Nanohybrids Material (Binhs) Of Deoxyribonucleic Acid Encapsulated Into Layered Double Hydroxide
title_short	Synthesis Of Bio-Inorganic Nanohybrids Material (Binhs) Of Deoxyribonucleic Acid Encapsulated Into Layered Double Hydroxide
title_full	Synthesis Of Bio-Inorganic Nanohybrids Material (Binhs) Of Deoxyribonucleic Acid Encapsulated Into Layered Double Hydroxide
title_fullStr	Synthesis Of Bio-Inorganic Nanohybrids Material (Binhs) Of Deoxyribonucleic Acid Encapsulated Into Layered Double Hydroxide
title_full_unstemmed	Synthesis Of Bio-Inorganic Nanohybrids Material (Binhs) Of Deoxyribonucleic Acid Encapsulated Into Layered Double Hydroxide
title_sort	synthesis of bio-inorganic nanohybrids material (binhs) of deoxyribonucleic acid encapsulated into layered double hydroxide
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Biotechnology and Biomolecular sciences
publishDate	2008
url	http://psasir.upm.edu.my/id/eprint/4938/1/FBSB_2008_12a.pdf
_version_	1747810314579083264

Synthesis Of Bio-Inorganic Nanohybrids Material (Binhs) Of Deoxyribonucleic Acid Encapsulated Into Layered Double Hydroxide

Similar Items