Comparison of sex hormone manipulation effects during perinatal period on mRNA expression of Slc9a4, Nr3c2, Htr5b and Mas1, in hippocampus and frontal cortex of male and female rats).

Spatial memory and the ability to navigate through space are known as sexually dimorphic traits among mammals, and numerous studies have shown that these traits can be altered by means of sex hormone manipulation. Hippocampus, the main organ involved in this kind of memory, has specific signature g...

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Main Author:	Karimi, Benyamin
Format:	Thesis
Language:	English
Published:	2013
Subjects:	Reproduction Animal breeding Neural stem cells
Online Access:	http://psasir.upm.edu.my/id/eprint/49394/1/FP%202013%2043RR.pdf
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id	my-upm-ir.49394
record_format	uketd_dc
spelling	my-upm-ir.493942016-11-17T03:37:44Z Comparison of sex hormone manipulation effects during perinatal period on mRNA expression of Slc9a4, Nr3c2, Htr5b and Mas1, in hippocampus and frontal cortex of male and female rats). 2013-11 Karimi, Benyamin Spatial memory and the ability to navigate through space are known as sexually dimorphic traits among mammals, and numerous studies have shown that these traits can be altered by means of sex hormone manipulation. Hippocampus, the main organ involved in this kind of memory, has specific signature genes, with high expression level compared to other regions of the brain. Based on their expression levels and the role that products of these genes can play in processes like signal transduction, mediation of hormone effects and long term potentiation, these genes can be considered as genes necessary for routine tasks of hippocampus. Male and female rat pups were injected with estradiol and testosterone, respectively, at early stage of their lives to examine the effect of sex hormone manipulation on mRNA expression of Slc9a4, Nr3c2, Htr5b and Mas1,using comparative quantitative real-time polymerase chain reaction. Another parallel study was also conducted, using another set of animals, to test the spatial ability after administration of sex hormones under identical conditions. The results showed that expressions of these genes are strongly influenced by sex hormones in both the frontal cortex and hippocampus, especially in male hippocampus, in which expression of all genes were up-regulated. Htr5b was the only gene that was affected only in the males. Expression of Mas1, contrary to expectations, showed stronger changes in its expression in cortex than in hippocampus. Nr3c2 was down regulated in all samples but up regulated in male hippocampus; Slc9a4 also showed a huge up-regulation in male hippocampus compared to other samples. It was also evident from the Morris Water Maze test results that sex hormone manipulation significantly alters the ability in spatial memory and navigation. Reproduction Animal breeding Neural stem cells 2013-11 Thesis http://psasir.upm.edu.my/id/eprint/49394/ http://psasir.upm.edu.my/id/eprint/49394/1/FP%202013%2043RR.pdf application/pdf en public masters Universiti Putra Malaysia Reproduction Animal breeding Neural stem cells
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
topic	Reproduction Animal breeding Neural stem cells
spellingShingle	Reproduction Animal breeding Neural stem cells Karimi, Benyamin Comparison of sex hormone manipulation effects during perinatal period on mRNA expression of Slc9a4, Nr3c2, Htr5b and Mas1, in hippocampus and frontal cortex of male and female rats).
description	Spatial memory and the ability to navigate through space are known as sexually dimorphic traits among mammals, and numerous studies have shown that these traits can be altered by means of sex hormone manipulation. Hippocampus, the main organ involved in this kind of memory, has specific signature genes, with high expression level compared to other regions of the brain. Based on their expression levels and the role that products of these genes can play in processes like signal transduction, mediation of hormone effects and long term potentiation, these genes can be considered as genes necessary for routine tasks of hippocampus. Male and female rat pups were injected with estradiol and testosterone, respectively, at early stage of their lives to examine the effect of sex hormone manipulation on mRNA expression of Slc9a4, Nr3c2, Htr5b and Mas1,using comparative quantitative real-time polymerase chain reaction. Another parallel study was also conducted, using another set of animals, to test the spatial ability after administration of sex hormones under identical conditions. The results showed that expressions of these genes are strongly influenced by sex hormones in both the frontal cortex and hippocampus, especially in male hippocampus, in which expression of all genes were up-regulated. Htr5b was the only gene that was affected only in the males. Expression of Mas1, contrary to expectations, showed stronger changes in its expression in cortex than in hippocampus. Nr3c2 was down regulated in all samples but up regulated in male hippocampus; Slc9a4 also showed a huge up-regulation in male hippocampus compared to other samples. It was also evident from the Morris Water Maze test results that sex hormone manipulation significantly alters the ability in spatial memory and navigation.
format	Thesis
qualification_level	Master's degree
author	Karimi, Benyamin
author_facet	Karimi, Benyamin
author_sort	Karimi, Benyamin
title	Comparison of sex hormone manipulation effects during perinatal period on mRNA expression of Slc9a4, Nr3c2, Htr5b and Mas1, in hippocampus and frontal cortex of male and female rats).
title_short	Comparison of sex hormone manipulation effects during perinatal period on mRNA expression of Slc9a4, Nr3c2, Htr5b and Mas1, in hippocampus and frontal cortex of male and female rats).
title_full	Comparison of sex hormone manipulation effects during perinatal period on mRNA expression of Slc9a4, Nr3c2, Htr5b and Mas1, in hippocampus and frontal cortex of male and female rats).
title_fullStr	Comparison of sex hormone manipulation effects during perinatal period on mRNA expression of Slc9a4, Nr3c2, Htr5b and Mas1, in hippocampus and frontal cortex of male and female rats).
title_full_unstemmed	Comparison of sex hormone manipulation effects during perinatal period on mRNA expression of Slc9a4, Nr3c2, Htr5b and Mas1, in hippocampus and frontal cortex of male and female rats).
title_sort	comparison of sex hormone manipulation effects during perinatal period on mrna expression of slc9a4, nr3c2, htr5b and mas1, in hippocampus and frontal cortex of male and female rats).
granting_institution	Universiti Putra Malaysia
publishDate	2013
url	http://psasir.upm.edu.my/id/eprint/49394/1/FP%202013%2043RR.pdf
_version_	1747812013362380800

Comparison of sex hormone manipulation effects during perinatal period on mRNA expression of Slc9a4, Nr3c2, Htr5b and Mas1, in hippocampus and frontal cortex of male and female rats).

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