Degradation Of Shrimp Waste Using Chitinase Produced By Trichoderma Virens UKM1 Through Media Selection

The current increase in the amount of seafood wastes produced by the shrimp industry has led to the finding of new methods for shrimp waste disposal or its recycle. Chitinaseproducing fungi is investigated to be used as a biocontrol agents in particular their potential for waste degradation of shrim...

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Main Author:	Teoh, Lay Sin
Format:	Thesis
Language:	English English
Published:	2008
Subjects:	Shrimps - Chitimase - Trichoderma
Online Access:	http://psasir.upm.edu.my/id/eprint/4947/1/FBSB_2008_10.pdf
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id	my-upm-ir.4947
record_format	uketd_dc
spelling	my-upm-ir.49472013-05-27T07:19:20Z Degradation Of Shrimp Waste Using Chitinase Produced By Trichoderma Virens UKM1 Through Media Selection 2008 Teoh, Lay Sin The current increase in the amount of seafood wastes produced by the shrimp industry has led to the finding of new methods for shrimp waste disposal or its recycle. Chitinaseproducing fungi is investigated to be used as a biocontrol agents in particular their potential for waste degradation of shrimps and crab shells from fishing industry. Locally isolated Trichoderma virens UKM1 expressing chitinases enzyme was used in this study. Three types of media were selected namely Medium 4, Medium 5 and Trichoderma minimal medium (TMM) for the degradation study. The substrate used was colloidal chitin as control substrate, ground and unground sun-dried shrimp shell wastes. Scanning Electron Microscopy (SEM) studies showed penetration of fungus mycelium into the colloidal chitin as compared to ground and unground sun-dried shrimp shells. Trichoderma virens UKM1 in TMM produced the less biomass and highest chitinase enzyme and in Medium 4 the protein concentration is the highest if compared with other media used. The results obtained suggested that colloidal chitin was the best carbon source for modelling the degradation of chitin materials. Stereo microscope studies showed that the mechanism of chitinous materials degradation by the chitinase enzyme is via sequential degradation. Shrimp wastes were further analysed for end products in the crude media using High Performance Liquid Chromatography (HPLC). Results showed that the Trichoderma virens UKM1 secretes a significant amount of exochitinase compared to endochitinase by the identification of monomeric N- acetylglucosamine (NAG) from the chitinous substrate. Trichoderma virens UKM1 produced more NAG using TMM with colloidal chitin as the substrate. Shrimps - Chitimase - Trichoderma 2008 Thesis http://psasir.upm.edu.my/id/eprint/4947/ http://psasir.upm.edu.my/id/eprint/4947/1/FBSB_2008_10.pdf application/pdf en public masters Universiti Putra Malaysia Shrimps - Chitimase - Trichoderma Faculty of Biotechnology and Biomolecular sciences English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic	Shrimps - Chitimase - Trichoderma
spellingShingle	Shrimps - Chitimase - Trichoderma Teoh, Lay Sin Degradation Of Shrimp Waste Using Chitinase Produced By Trichoderma Virens UKM1 Through Media Selection
description	The current increase in the amount of seafood wastes produced by the shrimp industry has led to the finding of new methods for shrimp waste disposal or its recycle. Chitinaseproducing fungi is investigated to be used as a biocontrol agents in particular their potential for waste degradation of shrimps and crab shells from fishing industry. Locally isolated Trichoderma virens UKM1 expressing chitinases enzyme was used in this study. Three types of media were selected namely Medium 4, Medium 5 and Trichoderma minimal medium (TMM) for the degradation study. The substrate used was colloidal chitin as control substrate, ground and unground sun-dried shrimp shell wastes. Scanning Electron Microscopy (SEM) studies showed penetration of fungus mycelium into the colloidal chitin as compared to ground and unground sun-dried shrimp shells. Trichoderma virens UKM1 in TMM produced the less biomass and highest chitinase enzyme and in Medium 4 the protein concentration is the highest if compared with other media used. The results obtained suggested that colloidal chitin was the best carbon source for modelling the degradation of chitin materials. Stereo microscope studies showed that the mechanism of chitinous materials degradation by the chitinase enzyme is via sequential degradation. Shrimp wastes were further analysed for end products in the crude media using High Performance Liquid Chromatography (HPLC). Results showed that the Trichoderma virens UKM1 secretes a significant amount of exochitinase compared to endochitinase by the identification of monomeric N- acetylglucosamine (NAG) from the chitinous substrate. Trichoderma virens UKM1 produced more NAG using TMM with colloidal chitin as the substrate.
format	Thesis
qualification_level	Master's degree
author	Teoh, Lay Sin
author_facet	Teoh, Lay Sin
author_sort	Teoh, Lay Sin
title	Degradation Of Shrimp Waste Using Chitinase Produced By Trichoderma Virens UKM1 Through Media Selection
title_short	Degradation Of Shrimp Waste Using Chitinase Produced By Trichoderma Virens UKM1 Through Media Selection
title_full	Degradation Of Shrimp Waste Using Chitinase Produced By Trichoderma Virens UKM1 Through Media Selection
title_fullStr	Degradation Of Shrimp Waste Using Chitinase Produced By Trichoderma Virens UKM1 Through Media Selection
title_full_unstemmed	Degradation Of Shrimp Waste Using Chitinase Produced By Trichoderma Virens UKM1 Through Media Selection
title_sort	degradation of shrimp waste using chitinase produced by trichoderma virens ukm1 through media selection
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Biotechnology and Biomolecular sciences
publishDate	2008
url	http://psasir.upm.edu.my/id/eprint/4947/1/FBSB_2008_10.pdf
_version_	1747810316540968960

Degradation Of Shrimp Waste Using Chitinase Produced By Trichoderma Virens UKM1 Through Media Selection

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