Single nucleotide polymorphism (SNP45) and plasma adiponectin level among antenatal women in selected polyclinics in Selangor

Gestational diabetes mellitus according to World Health Organization definition is the carbohydrate intolerance resulting in hyperglycemia of variable severity with onset or first diagnosed during pregnancy. Chronic insulin resistance appears to be the core of pathophysiology of gestational diabetes...

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Main Author:	Low, Chen Fei
Format:	Thesis
Language:	English
Published:	2009
Subjects:	Polymorphism Single Nucleotide - genetics
Online Access:	http://psasir.upm.edu.my/id/eprint/49941/1/FPSK%28m%29%202009%2016RR.pdf
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spelling	my-upm-ir.499412017-05-04T09:34:09Z Single nucleotide polymorphism (SNP45) and plasma adiponectin level among antenatal women in selected polyclinics in Selangor 2009-12 Low, Chen Fei Gestational diabetes mellitus according to World Health Organization definition is the carbohydrate intolerance resulting in hyperglycemia of variable severity with onset or first diagnosed during pregnancy. Chronic insulin resistance appears to be the core of pathophysiology of gestational diabetes mellitus that actuates during mid-pregnancy and progress through third trimester. Adiponectin which is one of the adipocyte-secreted hormones was recently been reported to be associated with insulin resistance, and accumulating literature is included gestational diabetes mellitus. Adiponectin has been identified to posses anti-atherogenic, insulin-sensiting as well as lipid-oxidatin enhancing properties. Single nucleotide polymorphisms in adiponectin gene had shown significant correlation with its plasma concentration and to the extent of risk of metabolic disorder. However, dissimilar findings had been reported from different populations and ethnics group. This present study aimed to identify the association of adiponectin single nucleotide polymorphism (SNP45) with gestational diabetes mellitus; and compare various parameters between gestational diabetic and normal patients during early of pregnancy. A total number of 104 antenatal patients from three different polyclinics; Polyclinic Bangi, Polyclinic Kajang and Polyclinic Seri Kembangan; participated in this study, and patients were in early trimester of pregnancy at first visits to antenatal clinic. Subsequently, the respondents were followed up and 79 patients retained in the following study, comprised of 53 normal and 26 gestational diabetic patients. All biochemical tests were conducted using blood plasma and patients” deoxyribonucleic acid was extracted from whole blood. Adiponectin SNP45 was screened using restriction enzyme SmaI. Findings from this present study revealed that gestational diabetic patients exhibit a significantly lower plasma adiponectin level as compared to normal patients (P<0.05) during early trimester. Besides, no significant difference was observed in patients’ plasma fructosamine level and hemoglobin A1c percentage between gestational diabetic and normal patients during the early trimester. Analysis of adiponectin SNP45 revealed a significant association with gestational diabetes mellitus (P = 0.004); and that G allele in SNP45 is significantly associated with gestational diabetes (P= 0.038). A significant lower level of plasma adiponectin in gestational diabetic patients that carry TG/GG genotype in adiponectin SNP45 as compared to normal patients that carry TT genotype was found, revealing a possible role of adiponectin SNP45 in circulating plasma adiponectin concentrations. In normal patients, patients that carry TG/GG genotype exhibit significant higher BMI compared to patients that carry TT genotype. However, this observation was not found in gestational diabetic patients. Therefore, it has been concluded that gestational diabetic patients exhibit lower level of plasma adiponectin during early trimester; and adiponectin SPN45 is associated with gestational diabetes mellitus in the sample population from this present study. Polymorphism, Single Nucleotide - genetics 2009-12 Thesis http://psasir.upm.edu.my/id/eprint/49941/ http://psasir.upm.edu.my/id/eprint/49941/1/FPSK%28m%29%202009%2016RR.pdf application/pdf en public masters Universiti Putra Malaysia Polymorphism, Single Nucleotide - genetics
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
topic	Polymorphism Single Nucleotide - genetics
spellingShingle	Polymorphism Single Nucleotide - genetics Low, Chen Fei Single nucleotide polymorphism (SNP45) and plasma adiponectin level among antenatal women in selected polyclinics in Selangor
description	Gestational diabetes mellitus according to World Health Organization definition is the carbohydrate intolerance resulting in hyperglycemia of variable severity with onset or first diagnosed during pregnancy. Chronic insulin resistance appears to be the core of pathophysiology of gestational diabetes mellitus that actuates during mid-pregnancy and progress through third trimester. Adiponectin which is one of the adipocyte-secreted hormones was recently been reported to be associated with insulin resistance, and accumulating literature is included gestational diabetes mellitus. Adiponectin has been identified to posses anti-atherogenic, insulin-sensiting as well as lipid-oxidatin enhancing properties. Single nucleotide polymorphisms in adiponectin gene had shown significant correlation with its plasma concentration and to the extent of risk of metabolic disorder. However, dissimilar findings had been reported from different populations and ethnics group. This present study aimed to identify the association of adiponectin single nucleotide polymorphism (SNP45) with gestational diabetes mellitus; and compare various parameters between gestational diabetic and normal patients during early of pregnancy. A total number of 104 antenatal patients from three different polyclinics; Polyclinic Bangi, Polyclinic Kajang and Polyclinic Seri Kembangan; participated in this study, and patients were in early trimester of pregnancy at first visits to antenatal clinic. Subsequently, the respondents were followed up and 79 patients retained in the following study, comprised of 53 normal and 26 gestational diabetic patients. All biochemical tests were conducted using blood plasma and patients” deoxyribonucleic acid was extracted from whole blood. Adiponectin SNP45 was screened using restriction enzyme SmaI. Findings from this present study revealed that gestational diabetic patients exhibit a significantly lower plasma adiponectin level as compared to normal patients (P<0.05) during early trimester. Besides, no significant difference was observed in patients’ plasma fructosamine level and hemoglobin A1c percentage between gestational diabetic and normal patients during the early trimester. Analysis of adiponectin SNP45 revealed a significant association with gestational diabetes mellitus (P = 0.004); and that G allele in SNP45 is significantly associated with gestational diabetes (P= 0.038). A significant lower level of plasma adiponectin in gestational diabetic patients that carry TG/GG genotype in adiponectin SNP45 as compared to normal patients that carry TT genotype was found, revealing a possible role of adiponectin SNP45 in circulating plasma adiponectin concentrations. In normal patients, patients that carry TG/GG genotype exhibit significant higher BMI compared to patients that carry TT genotype. However, this observation was not found in gestational diabetic patients. Therefore, it has been concluded that gestational diabetic patients exhibit lower level of plasma adiponectin during early trimester; and adiponectin SPN45 is associated with gestational diabetes mellitus in the sample population from this present study.
format	Thesis
qualification_level	Master's degree
author	Low, Chen Fei
author_facet	Low, Chen Fei
author_sort	Low, Chen Fei
title	Single nucleotide polymorphism (SNP45) and plasma adiponectin level among antenatal women in selected polyclinics in Selangor
title_short	Single nucleotide polymorphism (SNP45) and plasma adiponectin level among antenatal women in selected polyclinics in Selangor
title_full	Single nucleotide polymorphism (SNP45) and plasma adiponectin level among antenatal women in selected polyclinics in Selangor
title_fullStr	Single nucleotide polymorphism (SNP45) and plasma adiponectin level among antenatal women in selected polyclinics in Selangor
title_full_unstemmed	Single nucleotide polymorphism (SNP45) and plasma adiponectin level among antenatal women in selected polyclinics in Selangor
title_sort	single nucleotide polymorphism (snp45) and plasma adiponectin level among antenatal women in selected polyclinics in selangor
granting_institution	Universiti Putra Malaysia
publishDate	2009
url	http://psasir.upm.edu.my/id/eprint/49941/1/FPSK%28m%29%202009%2016RR.pdf
_version_	1747812024876793856

Single nucleotide polymorphism (SNP45) and plasma adiponectin level among antenatal women in selected polyclinics in Selangor

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