Tolerance of Trichoderma Atroviride (Karst.) Isolated From Freshwater Ecosystems towards Copper and Zinc

Isolation of microfungi from sediment samples of Kuyoh River Industrial Area, Kuyoh River Residential Area and Sri Serdang Lake was done on Rose Bengal Agar (RBA). The fungi were exposed to increased concentrations of Cu2+ (5 to 600 mg/L) and Zn2+ (5 to 6000 mg/L) on Potato Dextrose Agar (PDA) to fi...

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Main Author:	Yazdani, Mazyar
Format:	Thesis
Language:	English English
Published:	2008
Subjects:	Trichoderma - Malaysia - Case studies Bioremediation Bioremediator
Online Access:	http://psasir.upm.edu.my/id/eprint/5139/1/FS_2008_27.pdf
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record_format	uketd_dc
spelling	my-upm-ir.51392013-05-27T07:20:39Z Tolerance of Trichoderma Atroviride (Karst.) Isolated From Freshwater Ecosystems towards Copper and Zinc 2008 Yazdani, Mazyar Isolation of microfungi from sediment samples of Kuyoh River Industrial Area, Kuyoh River Residential Area and Sri Serdang Lake was done on Rose Bengal Agar (RBA). The fungi were exposed to increased concentrations of Cu2+ (5 to 600 mg/L) and Zn2+ (5 to 6000 mg/L) on Potato Dextrose Agar (PDA) to find the most tolerant isolate. The highest tolerance to both elements was shown by Trichoderma atroviride. Further studies on growth rate, uptake capacity and localization of Cu2+ and Zn2+ were made by using Potato Dextrose Broth (PDB) incorporated with 25 to 300 mg/L of Cu2+ and 500 to 1000 mg/L of Zn2+. This study found that the uptake capacity of T. atroviride for Cu2+ ranged from 0.77 to 11.20 mg/g between 25 to 300 mg/L in the liquid medium while that for Zn2+ ranged from 18.1 to 26.7 mg/g between 500 to 1000 mg/L in the liquid medium. The isolate showed that 50.3 to 85.4 % adsorption and 9.6 to 47.1 % absorption for Cu2+ and 47.6 to 64% adsorption and 30.4 to 45.1% absorption for Zn2+. Based on the present studies, 2.7 to 5 % of Cu2+ and 5.7 to 7.4 % of Zn2+removal was observed due to biomass washing. The mean levels of Cu2+ in the surface sediment of the three mentioned sites were 347.64 μg/g, 32.04 μg/g and 21.71 μg/g while the mean levels of Zn2+were 219.75 μg/g, 140.64 μg/g and 85.10 μg/g, respectively. This study suggests that T. atroviride is a potential bioremediator of Cu2+ and Zn2+. However, further studies are needed to confirm its practical use as a bioremediating agent. Trichoderma - Malaysia - Case studies Bioremediation Bioremediator 2008 Thesis http://psasir.upm.edu.my/id/eprint/5139/ http://psasir.upm.edu.my/id/eprint/5139/1/FS_2008_27.pdf application/pdf en public masters Universiti Putra Malaysia Trichoderma - Malaysia - Case studies Bioremediation Bioremediator Science English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic	Trichoderma - Malaysia - Case studies Bioremediation Bioremediator
spellingShingle	Trichoderma - Malaysia - Case studies Bioremediation Bioremediator Yazdani, Mazyar Tolerance of Trichoderma Atroviride (Karst.) Isolated From Freshwater Ecosystems towards Copper and Zinc
description	Isolation of microfungi from sediment samples of Kuyoh River Industrial Area, Kuyoh River Residential Area and Sri Serdang Lake was done on Rose Bengal Agar (RBA). The fungi were exposed to increased concentrations of Cu2+ (5 to 600 mg/L) and Zn2+ (5 to 6000 mg/L) on Potato Dextrose Agar (PDA) to find the most tolerant isolate. The highest tolerance to both elements was shown by Trichoderma atroviride. Further studies on growth rate, uptake capacity and localization of Cu2+ and Zn2+ were made by using Potato Dextrose Broth (PDB) incorporated with 25 to 300 mg/L of Cu2+ and 500 to 1000 mg/L of Zn2+. This study found that the uptake capacity of T. atroviride for Cu2+ ranged from 0.77 to 11.20 mg/g between 25 to 300 mg/L in the liquid medium while that for Zn2+ ranged from 18.1 to 26.7 mg/g between 500 to 1000 mg/L in the liquid medium. The isolate showed that 50.3 to 85.4 % adsorption and 9.6 to 47.1 % absorption for Cu2+ and 47.6 to 64% adsorption and 30.4 to 45.1% absorption for Zn2+. Based on the present studies, 2.7 to 5 % of Cu2+ and 5.7 to 7.4 % of Zn2+removal was observed due to biomass washing. The mean levels of Cu2+ in the surface sediment of the three mentioned sites were 347.64 μg/g, 32.04 μg/g and 21.71 μg/g while the mean levels of Zn2+were 219.75 μg/g, 140.64 μg/g and 85.10 μg/g, respectively. This study suggests that T. atroviride is a potential bioremediator of Cu2+ and Zn2+. However, further studies are needed to confirm its practical use as a bioremediating agent.
format	Thesis
qualification_level	Master's degree
author	Yazdani, Mazyar
author_facet	Yazdani, Mazyar
author_sort	Yazdani, Mazyar
title	Tolerance of Trichoderma Atroviride (Karst.) Isolated From Freshwater Ecosystems towards Copper and Zinc
title_short	Tolerance of Trichoderma Atroviride (Karst.) Isolated From Freshwater Ecosystems towards Copper and Zinc
title_full	Tolerance of Trichoderma Atroviride (Karst.) Isolated From Freshwater Ecosystems towards Copper and Zinc
title_fullStr	Tolerance of Trichoderma Atroviride (Karst.) Isolated From Freshwater Ecosystems towards Copper and Zinc
title_full_unstemmed	Tolerance of Trichoderma Atroviride (Karst.) Isolated From Freshwater Ecosystems towards Copper and Zinc
title_sort	tolerance of trichoderma atroviride (karst.) isolated from freshwater ecosystems towards copper and zinc
granting_institution	Universiti Putra Malaysia
granting_department	Science
publishDate	2008
url	http://psasir.upm.edu.my/id/eprint/5139/1/FS_2008_27.pdf
_version_	1747810359629053952

Tolerance of Trichoderma Atroviride (Karst.) Isolated From Freshwater Ecosystems towards Copper and Zinc

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