Characterization of Vibrio Parahaemolyticus Isolated From Local Cockles (Anadara Granosa) From Tanjung Karang, Kuala Selangor

Characterization of Vibrio Parahaemolyticus Isolated From Local Cockles (Anadara Granosa) From Tanjung Karang, Kuala Selangor

Vibrio parahaemolyticus is widespread in occurrence and has been recognized as a cause of gastroenteritis related to consumption of raw or improperly cooked seafood. Outbreaks of V. parahaemolyticus food poisoning are most common in Taiwan, Japan and Southeast Asia. In this study, V. parahaemolyticu...

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Main Author: Bilung, Lesley Maurice
Format: Thesis
Language:English
English
Published: 2006
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Vibrio parahaemolyticus - Tanjung Karang - Selangor.
Online Access:http://psasir.upm.edu.my/id/eprint/5289/1/FSTM_2006_27.pdf
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spelling my-upm-ir.52892013-05-27T07:21:44Z Characterization of Vibrio Parahaemolyticus Isolated From Local Cockles (Anadara Granosa) From Tanjung Karang, Kuala Selangor 2006 Bilung, Lesley Maurice Vibrio parahaemolyticus is widespread in occurrence and has been recognized as a cause of gastroenteritis related to consumption of raw or improperly cooked seafood. Outbreaks of V. parahaemolyticus food poisoning are most common in Taiwan, Japan and Southeast Asia. In this study, V. parahaemolyticus was isolated from 62 of 100 (62%) samples of cockles (Anadara granosa) collected from Tanjong Karang, Kuala Selangor. A total of 62 strains were studied for the presence or absence of regulatory gene (toxR), virulence genes (tdh and trh), their antibiotic resistance, the occurrence of plasmids and their molecular fingerprints by Randomly Amplified Polymorphic DNA – Polymerase Chain Reaction (RAPD-PCR) and Enterobacterial Repetitive Intergenic Consensus - Polymerase Chain Reaction (ERIC-PCR) assays. All 62 strains were positive for the regulatory gene (toxR) of V. parahaemolyticus. The PCR analysis for the detection of tdh or trh genes showed two (3.2%) positive strains carrying tdh gene and eleven (17.7%) strains had trh gene. The MPN value for all samples was more than 1100 MPN/g. This study has shown that all strains were multiple resistant to three or more of the seventeen antibiotics tested with the MAR indices ranging from 0.58-0.94. All isolates of V. parahaemolyticus were highly resistant towards the antibiotics tested, except one strain that was sensitive towards norfloxacin. Plasmids were found in 80% of the strains analyzed and 18 different plasmid profiles were observed. The plasmid size ranged from 2.7 to more than 54 kb. Two molecular typing methods were used in this study to examine the genetic relatedness among the V. parahaemolyticus strains. In the analysis by RAPD-PCR and ERIC-PCR, the size of RAPD and ERIC fragments ranged from 0.25 to 10.0 kb with an average number of ten and eight bands, respectively. Sixty-two genotypes among the 62 V. parahaemolyticus strains were generated using RAPD and ERIC-PCR which indicates that the strains were very diverse. Hence, this study, demonstrated that the local cockles are potential source for pathogenic V. parahaemolyticus. Vibrio parahaemolyticus - Tanjung Karang - Selangor. 2006 Thesis http://psasir.upm.edu.my/id/eprint/5289/ http://psasir.upm.edu.my/id/eprint/5289/1/FSTM_2006_27.pdf application/pdf en public phd doctoral Universiti Putra Malaysia Vibrio parahaemolyticus - Tanjung Karang - Selangor. Food Science and Technology English
institution Universiti Putra Malaysia
collection PSAS Institutional Repository
language English
English
topic Vibrio parahaemolyticus - Tanjung Karang - Selangor.
spellingShingle Vibrio parahaemolyticus - Tanjung Karang - Selangor.


Bilung, Lesley Maurice
Characterization of Vibrio Parahaemolyticus Isolated From Local Cockles (Anadara Granosa) From Tanjung Karang, Kuala Selangor
description Vibrio parahaemolyticus is widespread in occurrence and has been recognized as a cause of gastroenteritis related to consumption of raw or improperly cooked seafood. Outbreaks of V. parahaemolyticus food poisoning are most common in Taiwan, Japan and Southeast Asia. In this study, V. parahaemolyticus was isolated from 62 of 100 (62%) samples of cockles (Anadara granosa) collected from Tanjong Karang, Kuala Selangor. A total of 62 strains were studied for the presence or absence of regulatory gene (toxR), virulence genes (tdh and trh), their antibiotic resistance, the occurrence of plasmids and their molecular fingerprints by Randomly Amplified Polymorphic DNA – Polymerase Chain Reaction (RAPD-PCR) and Enterobacterial Repetitive Intergenic Consensus - Polymerase Chain Reaction (ERIC-PCR) assays. All 62 strains were positive for the regulatory gene (toxR) of V. parahaemolyticus. The PCR analysis for the detection of tdh or trh genes showed two (3.2%) positive strains carrying tdh gene and eleven (17.7%) strains had trh gene. The MPN value for all samples was more than 1100 MPN/g. This study has shown that all strains were multiple resistant to three or more of the seventeen antibiotics tested with the MAR indices ranging from 0.58-0.94. All isolates of V. parahaemolyticus were highly resistant towards the antibiotics tested, except one strain that was sensitive towards norfloxacin. Plasmids were found in 80% of the strains analyzed and 18 different plasmid profiles were observed. The plasmid size ranged from 2.7 to more than 54 kb. Two molecular typing methods were used in this study to examine the genetic relatedness among the V. parahaemolyticus strains. In the analysis by RAPD-PCR and ERIC-PCR, the size of RAPD and ERIC fragments ranged from 0.25 to 10.0 kb with an average number of ten and eight bands, respectively. Sixty-two genotypes among the 62 V. parahaemolyticus strains were generated using RAPD and ERIC-PCR which indicates that the strains were very diverse. Hence, this study, demonstrated that the local cockles are potential source for pathogenic V. parahaemolyticus.
format Thesis
qualification_name Doctor of Philosophy (PhD.)
qualification_level Doctorate
author Bilung, Lesley Maurice
author_facet Bilung, Lesley Maurice
author_sort Bilung, Lesley Maurice
title Characterization of Vibrio Parahaemolyticus Isolated From Local Cockles (Anadara Granosa) From Tanjung Karang, Kuala Selangor
title_short Characterization of Vibrio Parahaemolyticus Isolated From Local Cockles (Anadara Granosa) From Tanjung Karang, Kuala Selangor
title_full Characterization of Vibrio Parahaemolyticus Isolated From Local Cockles (Anadara Granosa) From Tanjung Karang, Kuala Selangor
title_fullStr Characterization of Vibrio Parahaemolyticus Isolated From Local Cockles (Anadara Granosa) From Tanjung Karang, Kuala Selangor
title_full_unstemmed Characterization of Vibrio Parahaemolyticus Isolated From Local Cockles (Anadara Granosa) From Tanjung Karang, Kuala Selangor
title_sort characterization of vibrio parahaemolyticus isolated from local cockles (anadara granosa) from tanjung karang, kuala selangor
granting_institution Universiti Putra Malaysia
granting_department Food Science and Technology
publishDate 2006
url http://psasir.upm.edu.my/id/eprint/5289/1/FSTM_2006_27.pdf
_version_ 1747810391972380672

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