Identification and Transcript Analysis of Differentially Expressed Genes from Floral Organs of Pigeon Orchid (Dendrobium Crumenatum)

Dendrobium is a member of the Orchidaceae, which is one of the largest families of flowering plants. Over the centuries, orchid flowers have evolved with myriad forms and devices to attract particular pollinators. This led to the development of highly modified organs which not only contributed to...

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Main Author:	Ng, Boon Zean
Format:	Thesis
Language:	English English
Published:	2007
Subjects:	Dendrobium Orchids
Online Access:	http://psasir.upm.edu.my/id/eprint/5383/1/IB_2007_8.pdf
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id	my-upm-ir.5383
record_format	uketd_dc
spelling	my-upm-ir.53832013-05-27T07:22:25Z Identification and Transcript Analysis of Differentially Expressed Genes from Floral Organs of Pigeon Orchid (Dendrobium Crumenatum) 2007 Ng, Boon Zean Dendrobium is a member of the Orchidaceae, which is one of the largest families of flowering plants. Over the centuries, orchid flowers have evolved with myriad forms and devices to attract particular pollinators. This led to the development of highly modified organs which not only contributed to the morphological diversity of orchid flowers, the success of pollination for some, but it also provided opportunities for accessing gene functions. Plant reproductive biology broadly comprises a network of biological events that represents a continuum of developmental processes beginning with the development and eventually culminating in the death of flowers. Due to the complex processes and biological mechanisms involved, analyses using molecular tools provided an opportunity to study the molecular elements that underlies the reproductive biology of orchids. This preliminary study was initiated to identify genes that are differentially expressed and putatively involved in the reproductive biology of the Pigeon orchid. Genes showing differential expression among the sepal, petal, lip and column of the Pigeon orchid were targeted using a derivative of the differential display technique known as GeneFishingTM technology. Ten differentially expressed transcripts were identified where sequence analyses revealed most of the transcripts include genes that were previously uncharacterized in the orchid system. Three partial cDNA clones which encode for small heat shock protein (A1C1-8), pectin methylesterase enzyme (A3C1-1) and 14-3-3 protein (A8C1-9) were selected for expression studies. Comparative expression profiling of these clones in other organs of the Pigeon orchid such as roots, stems and developing flower buds 3-4 days before anthesis via quantitative real-time RT-PCR revealed all three clones may putatively exhibit flower-specific expression. The results from realtime RT-PCR strongly suggest that these transcripts may possibly be involved in reproduction-related processes based on its localization in the column of the Pigeon orchid flowers. Dendrobium Orchids 2007 Thesis http://psasir.upm.edu.my/id/eprint/5383/ http://psasir.upm.edu.my/id/eprint/5383/1/IB_2007_8.pdf application/pdf en public masters Universiti Putra Malaysia Dendrobium Orchids Institute Bioscience English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic	Dendrobium Orchids
spellingShingle	Dendrobium Orchids Ng, Boon Zean Identification and Transcript Analysis of Differentially Expressed Genes from Floral Organs of Pigeon Orchid (Dendrobium Crumenatum)
description	Dendrobium is a member of the Orchidaceae, which is one of the largest families of flowering plants. Over the centuries, orchid flowers have evolved with myriad forms and devices to attract particular pollinators. This led to the development of highly modified organs which not only contributed to the morphological diversity of orchid flowers, the success of pollination for some, but it also provided opportunities for accessing gene functions. Plant reproductive biology broadly comprises a network of biological events that represents a continuum of developmental processes beginning with the development and eventually culminating in the death of flowers. Due to the complex processes and biological mechanisms involved, analyses using molecular tools provided an opportunity to study the molecular elements that underlies the reproductive biology of orchids. This preliminary study was initiated to identify genes that are differentially expressed and putatively involved in the reproductive biology of the Pigeon orchid. Genes showing differential expression among the sepal, petal, lip and column of the Pigeon orchid were targeted using a derivative of the differential display technique known as GeneFishingTM technology. Ten differentially expressed transcripts were identified where sequence analyses revealed most of the transcripts include genes that were previously uncharacterized in the orchid system. Three partial cDNA clones which encode for small heat shock protein (A1C1-8), pectin methylesterase enzyme (A3C1-1) and 14-3-3 protein (A8C1-9) were selected for expression studies. Comparative expression profiling of these clones in other organs of the Pigeon orchid such as roots, stems and developing flower buds 3-4 days before anthesis via quantitative real-time RT-PCR revealed all three clones may putatively exhibit flower-specific expression. The results from realtime RT-PCR strongly suggest that these transcripts may possibly be involved in reproduction-related processes based on its localization in the column of the Pigeon orchid flowers.
format	Thesis
qualification_level	Master's degree
author	Ng, Boon Zean
author_facet	Ng, Boon Zean
author_sort	Ng, Boon Zean
title	Identification and Transcript Analysis of Differentially Expressed Genes from Floral Organs of Pigeon Orchid (Dendrobium Crumenatum)
title_short	Identification and Transcript Analysis of Differentially Expressed Genes from Floral Organs of Pigeon Orchid (Dendrobium Crumenatum)
title_full	Identification and Transcript Analysis of Differentially Expressed Genes from Floral Organs of Pigeon Orchid (Dendrobium Crumenatum)
title_fullStr	Identification and Transcript Analysis of Differentially Expressed Genes from Floral Organs of Pigeon Orchid (Dendrobium Crumenatum)
title_full_unstemmed	Identification and Transcript Analysis of Differentially Expressed Genes from Floral Organs of Pigeon Orchid (Dendrobium Crumenatum)
title_sort	identification and transcript analysis of differentially expressed genes from floral organs of pigeon orchid (dendrobium crumenatum)
granting_institution	Universiti Putra Malaysia
granting_department	Institute Bioscience
publishDate	2007
url	http://psasir.upm.edu.my/id/eprint/5383/1/IB_2007_8.pdf
_version_	1747810412338872320

Identification and Transcript Analysis of Differentially Expressed Genes from Floral Organs of Pigeon Orchid (Dendrobium Crumenatum)

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