Biotransformation of Zerumbone and Goniothalamin by Aspergillus Niger (Ftcc 5003) and Sclerotium Rolfsii (Local Isolated): Structural Elucidation and Biological Activities

Microbial transformation was carried out on three natural product compounds, namely zerumbone, goniothalamin and cardamonin, using the fungi Aspergillus niger and Sclerotium rolfsii. Aspergillus niger and Sclerotium rolfsii have successfully converted zerumbone into two products, namely 2,3-dihydroz...

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Main Author:	Chia, Poh Wai
Format:	Thesis
Language:	English English
Published:	2008
Subjects:	Biotransformation (Metabolism)
Online Access:	http://psasir.upm.edu.my/id/eprint/5390/1/IB_2008_2a.pdf
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id	my-upm-ir.5390
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spelling	my-upm-ir.53902013-05-27T07:22:29Z Biotransformation of Zerumbone and Goniothalamin by Aspergillus Niger (Ftcc 5003) and Sclerotium Rolfsii (Local Isolated): Structural Elucidation and Biological Activities 2008 Chia, Poh Wai Microbial transformation was carried out on three natural product compounds, namely zerumbone, goniothalamin and cardamonin, using the fungi Aspergillus niger and Sclerotium rolfsii. Aspergillus niger and Sclerotium rolfsii have successfully converted zerumbone into two products, namely 2,3-dihydrozerumbone and 6,7-epoxyzerumbone. Aspergillus niger and Sclerotium rolfsii also successfully biotransformed goniothalamin into 2,3-dihydrogoniothalamin. The trial carried out on cardamonin was unsuccessful due to the antimicrobial activity of the compound that inhibited the growth of the fungi. Sclerotium rolfsii is a newly discovered biotransformer able to carry out hydrogenation and epoxidation. Two microbial toxins, namely gliotoxin and bisdethiobis(methylthio)gliotoxin were isolated in the course of biotransformation of goniothalamin using A. niger and S. rolfsii cultured on PKC (Palm Kernel Cake) media. The toxins were not produced when the biotransformation was repeated using fungi cultured on standard glucose media. Thus, PKC was deduced to be conducive to the production of the toxins by the two fungi. In an attempt to isolate and purify marchantin A as a candidate for biotransformation studies, several compounds which included marchantin A, apigenin, luteolin and a mixture of stigmasterol and β-sitosterol were found.. A preliminary antiinflammatory and anticholinesterase activities screening of the isolated compounds (zerumbone, 2,3-dihydrozerumbone, 6,7-epoxyzerumbone, goniothalamin, 2,3-dihydrogoniothalamin, gliotoxin, bisdethiobis(methylthio)gliotoxin, apigenin, luteolin, marchantin A and mixture of β-sitosterol and stigmasterol, were also conducted. Luteolin exhibited strong inhibition of soybean lipoxygenase with an IC50 value of 6.25 μg/ml while zerumbone showed moderate inhibition against soybean lipoxygenase with an IC50 value of 22.83 μg/ml. The other compounds were inactive towards the enzyme. In the anticholinesterase inhibitory assay, zerumbone, 6,7-epoxyzerumbone, marchantin A, goniothalamin, apigenin and luteolin, as well as a mixture of β-sitosterol and stigmasterol were found to inhibit the enzyme. Biotransformation (Metabolism) 2008 Thesis http://psasir.upm.edu.my/id/eprint/5390/ http://psasir.upm.edu.my/id/eprint/5390/1/IB_2008_2a.pdf application/pdf en public masters Universiti Putra Malaysia Biotransformation (Metabolism) Institute Bioscience English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic	Biotransformation (Metabolism)
spellingShingle	Biotransformation (Metabolism) Chia, Poh Wai Biotransformation of Zerumbone and Goniothalamin by Aspergillus Niger (Ftcc 5003) and Sclerotium Rolfsii (Local Isolated): Structural Elucidation and Biological Activities
description	Microbial transformation was carried out on three natural product compounds, namely zerumbone, goniothalamin and cardamonin, using the fungi Aspergillus niger and Sclerotium rolfsii. Aspergillus niger and Sclerotium rolfsii have successfully converted zerumbone into two products, namely 2,3-dihydrozerumbone and 6,7-epoxyzerumbone. Aspergillus niger and Sclerotium rolfsii also successfully biotransformed goniothalamin into 2,3-dihydrogoniothalamin. The trial carried out on cardamonin was unsuccessful due to the antimicrobial activity of the compound that inhibited the growth of the fungi. Sclerotium rolfsii is a newly discovered biotransformer able to carry out hydrogenation and epoxidation. Two microbial toxins, namely gliotoxin and bisdethiobis(methylthio)gliotoxin were isolated in the course of biotransformation of goniothalamin using A. niger and S. rolfsii cultured on PKC (Palm Kernel Cake) media. The toxins were not produced when the biotransformation was repeated using fungi cultured on standard glucose media. Thus, PKC was deduced to be conducive to the production of the toxins by the two fungi. In an attempt to isolate and purify marchantin A as a candidate for biotransformation studies, several compounds which included marchantin A, apigenin, luteolin and a mixture of stigmasterol and β-sitosterol were found.. A preliminary antiinflammatory and anticholinesterase activities screening of the isolated compounds (zerumbone, 2,3-dihydrozerumbone, 6,7-epoxyzerumbone, goniothalamin, 2,3-dihydrogoniothalamin, gliotoxin, bisdethiobis(methylthio)gliotoxin, apigenin, luteolin, marchantin A and mixture of β-sitosterol and stigmasterol, were also conducted. Luteolin exhibited strong inhibition of soybean lipoxygenase with an IC50 value of 6.25 μg/ml while zerumbone showed moderate inhibition against soybean lipoxygenase with an IC50 value of 22.83 μg/ml. The other compounds were inactive towards the enzyme. In the anticholinesterase inhibitory assay, zerumbone, 6,7-epoxyzerumbone, marchantin A, goniothalamin, apigenin and luteolin, as well as a mixture of β-sitosterol and stigmasterol were found to inhibit the enzyme.
format	Thesis
qualification_level	Master's degree
author	Chia, Poh Wai
author_facet	Chia, Poh Wai
author_sort	Chia, Poh Wai
title	Biotransformation of Zerumbone and Goniothalamin by Aspergillus Niger (Ftcc 5003) and Sclerotium Rolfsii (Local Isolated): Structural Elucidation and Biological Activities
title_short	Biotransformation of Zerumbone and Goniothalamin by Aspergillus Niger (Ftcc 5003) and Sclerotium Rolfsii (Local Isolated): Structural Elucidation and Biological Activities
title_full	Biotransformation of Zerumbone and Goniothalamin by Aspergillus Niger (Ftcc 5003) and Sclerotium Rolfsii (Local Isolated): Structural Elucidation and Biological Activities
title_fullStr	Biotransformation of Zerumbone and Goniothalamin by Aspergillus Niger (Ftcc 5003) and Sclerotium Rolfsii (Local Isolated): Structural Elucidation and Biological Activities
title_full_unstemmed	Biotransformation of Zerumbone and Goniothalamin by Aspergillus Niger (Ftcc 5003) and Sclerotium Rolfsii (Local Isolated): Structural Elucidation and Biological Activities
title_sort	biotransformation of zerumbone and goniothalamin by aspergillus niger (ftcc 5003) and sclerotium rolfsii (local isolated): structural elucidation and biological activities
granting_institution	Universiti Putra Malaysia
granting_department	Institute Bioscience
publishDate	2008
url	http://psasir.upm.edu.my/id/eprint/5390/1/IB_2008_2a.pdf
_version_	1747810414065876992

Biotransformation of Zerumbone and Goniothalamin by Aspergillus Niger (Ftcc 5003) and Sclerotium Rolfsii (Local Isolated): Structural Elucidation and Biological Activities

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