Antioxidant Activity And Bioactive Compounds Of Selected Tropical Plants

Antioxidant does not only prevent food containing lipid and oils from becoming rancid, but it also provides protection against harmful free radical and has been strongly associated with reduced risk of chronic diseases such as cardiovascular diseases, cancer and diabetes. The antioxidant activity...

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Main Author: Mustafa, Roheeyati Ashikin
Format: Thesis
Language:English
Published: 2009
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Online Access:http://psasir.upm.edu.my/id/eprint/5667/1/A_FSTM_2009_20%20IR.pdf
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spelling my-upm-ir.56672022-01-03T04:11:46Z Antioxidant Activity And Bioactive Compounds Of Selected Tropical Plants 2009-09 Mustafa, Roheeyati Ashikin Antioxidant does not only prevent food containing lipid and oils from becoming rancid, but it also provides protection against harmful free radical and has been strongly associated with reduced risk of chronic diseases such as cardiovascular diseases, cancer and diabetes. The antioxidant activity of 21 selected tropical plant extracts was evaluated utilizing four different assays including conjugated diene, scavenging DPPH radical, TBA and chelating effect on ferrous ion. Bioactive compounds (total phenolics, flavonoids, β-carotene, lycopene, α-tocopherol and ascorbic acid) were also determined in the plants extracts. Results of the study showed that C.caudatus extract was highly effective in the prevention of conjugated diene formation and scavenging effect on DPPH radicals. On the other hand, C.asiatica and P.tetragonolobus extracts demonstrated excellent activity in inhibiting malondialdehyde (MDA) formation and chelating ability on ferrous ion. As expected, increasing the concentration of extracts used increased the antioxidant activity in all plants tested. The antioxidant activities of some of these plants were found to be as good as that of α- tocopherol and BHA (Butylated hydroxyanisole). Results of the study revealed that L.inermis extracts consisted of the highest content of phenols and then flavonoids (catechin, epicatechin and naringenin) compared to all the plants tested. On the other hand, excellent concentration of quercetin and ascorbic acid were identified in V.negundo extract whilst surprisingly, P.bleo extract showed high content of lycopene and α-tocopherol. Highest kaempferol and myricetin was found in S.grandiflora and C.asiatica extracts whilst luteolin and apigenin were predominantly found in P.cordifolia and K.galanga extracts. On the other hand, highest β-carotene content was found in G.procumbens extract. Excellent correlation (R2=0.8613) was found between radical scavenging activity and the total content of phenolic compounds. Similar correlation (R2=0.8430) was seen between radical scavenging activity and flavonoids compounds. The study suggested that phenolic compounds in particular, the flavonoids contribute to the antioxidant activity. Keywords: Antioxidant activity; tropical plants; bioactive compounds; phenolic compounds; ascorbic acid. Antioxidants - Case studies Bioactive compounds - Case studies Plant extracts - Case studies 2009-09 Thesis http://psasir.upm.edu.my/id/eprint/5667/ http://psasir.upm.edu.my/id/eprint/5667/1/A_FSTM_2009_20%20IR.pdf text en public masters Universiti Putra Malaysia Antioxidants - Case studies Bioactive compounds - Case studies Plant extracts - Case studies Food Science and Technology Abdul Hamid, Azizah
institution Universiti Putra Malaysia
collection PSAS Institutional Repository
language English
advisor Abdul Hamid, Azizah
topic Antioxidants - Case studies
Bioactive compounds - Case studies
Plant extracts - Case studies
spellingShingle Antioxidants - Case studies
Bioactive compounds - Case studies
Plant extracts - Case studies
Mustafa, Roheeyati Ashikin
Antioxidant Activity And Bioactive Compounds Of Selected Tropical Plants
description Antioxidant does not only prevent food containing lipid and oils from becoming rancid, but it also provides protection against harmful free radical and has been strongly associated with reduced risk of chronic diseases such as cardiovascular diseases, cancer and diabetes. The antioxidant activity of 21 selected tropical plant extracts was evaluated utilizing four different assays including conjugated diene, scavenging DPPH radical, TBA and chelating effect on ferrous ion. Bioactive compounds (total phenolics, flavonoids, β-carotene, lycopene, α-tocopherol and ascorbic acid) were also determined in the plants extracts. Results of the study showed that C.caudatus extract was highly effective in the prevention of conjugated diene formation and scavenging effect on DPPH radicals. On the other hand, C.asiatica and P.tetragonolobus extracts demonstrated excellent activity in inhibiting malondialdehyde (MDA) formation and chelating ability on ferrous ion. As expected, increasing the concentration of extracts used increased the antioxidant activity in all plants tested. The antioxidant activities of some of these plants were found to be as good as that of α- tocopherol and BHA (Butylated hydroxyanisole). Results of the study revealed that L.inermis extracts consisted of the highest content of phenols and then flavonoids (catechin, epicatechin and naringenin) compared to all the plants tested. On the other hand, excellent concentration of quercetin and ascorbic acid were identified in V.negundo extract whilst surprisingly, P.bleo extract showed high content of lycopene and α-tocopherol. Highest kaempferol and myricetin was found in S.grandiflora and C.asiatica extracts whilst luteolin and apigenin were predominantly found in P.cordifolia and K.galanga extracts. On the other hand, highest β-carotene content was found in G.procumbens extract. Excellent correlation (R2=0.8613) was found between radical scavenging activity and the total content of phenolic compounds. Similar correlation (R2=0.8430) was seen between radical scavenging activity and flavonoids compounds. The study suggested that phenolic compounds in particular, the flavonoids contribute to the antioxidant activity. Keywords: Antioxidant activity; tropical plants; bioactive compounds; phenolic compounds; ascorbic acid.
format Thesis
qualification_level Master's degree
author Mustafa, Roheeyati Ashikin
author_facet Mustafa, Roheeyati Ashikin
author_sort Mustafa, Roheeyati Ashikin
title Antioxidant Activity And Bioactive Compounds Of Selected Tropical Plants
title_short Antioxidant Activity And Bioactive Compounds Of Selected Tropical Plants
title_full Antioxidant Activity And Bioactive Compounds Of Selected Tropical Plants
title_fullStr Antioxidant Activity And Bioactive Compounds Of Selected Tropical Plants
title_full_unstemmed Antioxidant Activity And Bioactive Compounds Of Selected Tropical Plants
title_sort antioxidant activity and bioactive compounds of selected tropical plants
granting_institution Universiti Putra Malaysia
granting_department Food Science and Technology
publishDate 2009
url http://psasir.upm.edu.my/id/eprint/5667/1/A_FSTM_2009_20%20IR.pdf
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