Adhesion Properties Of Bifidobacterium Pseudocatenulatum G4 To Ht-29 Epithelium Cell Line

Bifidobacterium pseudocatenulatum G4 has been recently identified as a safe probiotic for incorporation into functional food for human consumption. Preliminary investigations showed that the probiotic candidate B. pseudocatenulatum G4 strain possesses the required criteria for a being successful pro...

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Main Author:	Suliman, Ali Kahtan
Format:	Thesis
Language:	English English
Published:	2009
Subjects:	Bifidobacterium - Case studies
Online Access:	http://psasir.upm.edu.my/id/eprint/5681/1/A_FSTM_2009_9.pdf
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id	my-upm-ir.5681
record_format	uketd_dc
spelling	my-upm-ir.56812013-05-27T07:24:25Z Adhesion Properties Of Bifidobacterium Pseudocatenulatum G4 To Ht-29 Epithelium Cell Line 2009 Suliman, Ali Kahtan Bifidobacterium pseudocatenulatum G4 has been recently identified as a safe probiotic for incorporation into functional food for human consumption. Preliminary investigations showed that the probiotic candidate B. pseudocatenulatum G4 strain possesses the required criteria for a being successful probiotic microorganism. Further enhancement of these criteria was undertaken by studying the adherence and inhibition properties potential of this probiotic candidate. Human colon carcinoma epithelium cell line HT-29 was used to evaluate the adherence of B. pseudocatenulatum G4, in simulated environmental factors of the colon, namely pH, calcium ions, and cholic acid. The effect of this strain on enhancing intestinal tract resistance to pathogenic Escherichia coli and Clostridium infections was examined. Three different assays were used in order to differentiate between the competition, exclusion, and displacement of the pathogens by B. pseudocatenulatum G4. The adherence ability of B. pseudocatenulatum G4 to HT-29 cell line was investigated as in vitro model. In addition the morphology observation of the organism was done by using scanning electron microscopy (SEM). The effect of human colon environmental factors on the adhesion quality was studied. The results showed that the highest adhesion was in the ascending and transverse acidic regions of the colon. Calcium was shown to increase, while cholic acid was shown to decrease the adhesion of B. pseudocatenulatum G4 to HT-29. The inhibitory effect of B. pseudocatenulatum G4 on the adherence of Escherichia coli O157:H7, Clostridium scindens and Clostridium hiranonis was demonstrated. A decrease in the number of adhering pathogens was observed. Bifidobacterium - Case studies 2009 Thesis http://psasir.upm.edu.my/id/eprint/5681/ http://psasir.upm.edu.my/id/eprint/5681/1/A_FSTM_2009_9.pdf application/pdf en public masters Universiti Putra Malaysia Bifidobacterium - Case studies Faculty: Food Science and Technology English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic	Bifidobacterium - Case studies
spellingShingle	Bifidobacterium - Case studies Suliman, Ali Kahtan Adhesion Properties Of Bifidobacterium Pseudocatenulatum G4 To Ht-29 Epithelium Cell Line
description	Bifidobacterium pseudocatenulatum G4 has been recently identified as a safe probiotic for incorporation into functional food for human consumption. Preliminary investigations showed that the probiotic candidate B. pseudocatenulatum G4 strain possesses the required criteria for a being successful probiotic microorganism. Further enhancement of these criteria was undertaken by studying the adherence and inhibition properties potential of this probiotic candidate. Human colon carcinoma epithelium cell line HT-29 was used to evaluate the adherence of B. pseudocatenulatum G4, in simulated environmental factors of the colon, namely pH, calcium ions, and cholic acid. The effect of this strain on enhancing intestinal tract resistance to pathogenic Escherichia coli and Clostridium infections was examined. Three different assays were used in order to differentiate between the competition, exclusion, and displacement of the pathogens by B. pseudocatenulatum G4. The adherence ability of B. pseudocatenulatum G4 to HT-29 cell line was investigated as in vitro model. In addition the morphology observation of the organism was done by using scanning electron microscopy (SEM). The effect of human colon environmental factors on the adhesion quality was studied. The results showed that the highest adhesion was in the ascending and transverse acidic regions of the colon. Calcium was shown to increase, while cholic acid was shown to decrease the adhesion of B. pseudocatenulatum G4 to HT-29. The inhibitory effect of B. pseudocatenulatum G4 on the adherence of Escherichia coli O157:H7, Clostridium scindens and Clostridium hiranonis was demonstrated. A decrease in the number of adhering pathogens was observed.
format	Thesis
qualification_level	Master's degree
author	Suliman, Ali Kahtan
author_facet	Suliman, Ali Kahtan
author_sort	Suliman, Ali Kahtan
title	Adhesion Properties Of Bifidobacterium Pseudocatenulatum G4 To Ht-29 Epithelium Cell Line
title_short	Adhesion Properties Of Bifidobacterium Pseudocatenulatum G4 To Ht-29 Epithelium Cell Line
title_full	Adhesion Properties Of Bifidobacterium Pseudocatenulatum G4 To Ht-29 Epithelium Cell Line
title_fullStr	Adhesion Properties Of Bifidobacterium Pseudocatenulatum G4 To Ht-29 Epithelium Cell Line
title_full_unstemmed	Adhesion Properties Of Bifidobacterium Pseudocatenulatum G4 To Ht-29 Epithelium Cell Line
title_sort	adhesion properties of bifidobacterium pseudocatenulatum g4 to ht-29 epithelium cell line
granting_institution	Universiti Putra Malaysia
granting_department	Faculty: Food Science and Technology
publishDate	2009
url	http://psasir.upm.edu.my/id/eprint/5681/1/A_FSTM_2009_9.pdf
_version_	1747810462648500224

Adhesion Properties Of Bifidobacterium Pseudocatenulatum G4 To Ht-29 Epithelium Cell Line

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