Ultrasound-Assisted Extraction of Phospholipids from Palm-Pressed Fiber

The extraction of phospholipids (PL) from palm-pressed fiber (PPF) using ultrasound technology was evaluated and quantified using high performance liquid chromatography coupled with an evaporative light scattering detector (HPLC-ELSD). The PL sample was treated with diol solid phase extraction (SPE)...

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Main Author:	Chua, Seong Chea
Format:	Thesis
Language:	English
Published:	2009
Subjects:	Phaspholipids - Fiberboard - Case studies
Online Access:	http://psasir.upm.edu.my/id/eprint/5686/1/A_FSTM_2009_10.pdf
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id	my-upm-ir.5686
record_format	uketd_dc
spelling	my-upm-ir.56862015-08-06T06:26:53Z Ultrasound-Assisted Extraction of Phospholipids from Palm-Pressed Fiber 2009 Chua, Seong Chea The extraction of phospholipids (PL) from palm-pressed fiber (PPF) using ultrasound technology was evaluated and quantified using high performance liquid chromatography coupled with an evaporative light scattering detector (HPLC-ELSD). The PL sample was treated with diol solid phase extraction (SPE) to achieve better separation and higher PL recovery by removing neutral lipids. The types of PL that were considered in the recovery study included phosphatidylethanolamine (PE), phosphatidylglycerol (PG), phosphatidylcholine (PC), and lyso-phosphatidylcholine (LPC). A central composite design (CCD) was employed to study the effect of ultrasound-assisted extraction (UAE) conditions, namely amplitude (20%-90%), cycle (0.2-1.0 W/s), and sonication time (5-30 min), on the PL extraction yield from PPF. The optimum parameters for PL extraction using the ultrasound system were 20% amplitude, 0.2 W/s cycle, and a sonication time of 30 min. Under these optimum conditions, the response values obtained for overall extraction efficiency and individual extraction yield of PE and PC were 110 mg/g, 12570, and 5426 mg/kg, respectively. In the purification of PL, it was observed that acetone can remove oil from the extract. Ethanol was used to fractionate PC and PI, as both PC and PI have different solubility in this solvent. In general, PC is more soluble in ethanol than PI. Therefore, the ethanol extract was the PC-enriched fraction and the ethanol precipitate was the PI-enriched fraction. The sample to ethanol ratio significantly (p < 0.05) affected the purity of PC in the PC-enriched fraction. The best ratio of sample/ethanol (g/mL) was 1:20, which resulted in the highest percentage of the PC fraction, with the highest PC level in the PC fraction and the highest PI level in the PI fraction. The PC-enriched fraction contained 45% PC, 2% PE and 5% PI based on the total extract content, whereas the PI-enriched fraction contained 13% PC, 8% PE, and 60% PI. Phaspholipids - Fiberboard - Case studies 2009 Thesis http://psasir.upm.edu.my/id/eprint/5686/ http://psasir.upm.edu.my/id/eprint/5686/1/A_FSTM_2009_10.pdf application/pdf en public masters Universiti Putra Malaysia Phaspholipids - Fiberboard - Case studies Faculty of Food Science and Technology
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
topic	Phaspholipids - Fiberboard - Case studies
spellingShingle	Phaspholipids - Fiberboard - Case studies Chua, Seong Chea Ultrasound-Assisted Extraction of Phospholipids from Palm-Pressed Fiber
description	The extraction of phospholipids (PL) from palm-pressed fiber (PPF) using ultrasound technology was evaluated and quantified using high performance liquid chromatography coupled with an evaporative light scattering detector (HPLC-ELSD). The PL sample was treated with diol solid phase extraction (SPE) to achieve better separation and higher PL recovery by removing neutral lipids. The types of PL that were considered in the recovery study included phosphatidylethanolamine (PE), phosphatidylglycerol (PG), phosphatidylcholine (PC), and lyso-phosphatidylcholine (LPC). A central composite design (CCD) was employed to study the effect of ultrasound-assisted extraction (UAE) conditions, namely amplitude (20%-90%), cycle (0.2-1.0 W/s), and sonication time (5-30 min), on the PL extraction yield from PPF. The optimum parameters for PL extraction using the ultrasound system were 20% amplitude, 0.2 W/s cycle, and a sonication time of 30 min. Under these optimum conditions, the response values obtained for overall extraction efficiency and individual extraction yield of PE and PC were 110 mg/g, 12570, and 5426 mg/kg, respectively. In the purification of PL, it was observed that acetone can remove oil from the extract. Ethanol was used to fractionate PC and PI, as both PC and PI have different solubility in this solvent. In general, PC is more soluble in ethanol than PI. Therefore, the ethanol extract was the PC-enriched fraction and the ethanol precipitate was the PI-enriched fraction. The sample to ethanol ratio significantly (p < 0.05) affected the purity of PC in the PC-enriched fraction. The best ratio of sample/ethanol (g/mL) was 1:20, which resulted in the highest percentage of the PC fraction, with the highest PC level in the PC fraction and the highest PI level in the PI fraction. The PC-enriched fraction contained 45% PC, 2% PE and 5% PI based on the total extract content, whereas the PI-enriched fraction contained 13% PC, 8% PE, and 60% PI.
format	Thesis
qualification_level	Master's degree
author	Chua, Seong Chea
author_facet	Chua, Seong Chea
author_sort	Chua, Seong Chea
title	Ultrasound-Assisted Extraction of Phospholipids from Palm-Pressed Fiber
title_short	Ultrasound-Assisted Extraction of Phospholipids from Palm-Pressed Fiber
title_full	Ultrasound-Assisted Extraction of Phospholipids from Palm-Pressed Fiber
title_fullStr	Ultrasound-Assisted Extraction of Phospholipids from Palm-Pressed Fiber
title_full_unstemmed	Ultrasound-Assisted Extraction of Phospholipids from Palm-Pressed Fiber
title_sort	ultrasound-assisted extraction of phospholipids from palm-pressed fiber
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Food Science and Technology
publishDate	2009
url	http://psasir.upm.edu.my/id/eprint/5686/1/A_FSTM_2009_10.pdf
_version_	1747810463872188416

Ultrasound-Assisted Extraction of Phospholipids from Palm-Pressed Fiber

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