Characterization and Antioxidant Activity of Phenolic Extracts from Oil Palm (Elaeis Guineensis) Fruits

The extracted oil palm fruit phenolics were analysed using spectrophotometry methods to obtain information on the different types of oil palm phenolics and their antioxidative activities. Different methods were used to extract soluble free (SFP), insoluble-bound (ISBP) and esterified (EFP) phenolics...

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Main Author:	Neo, Yun Ping
Format:	Thesis
Language:	English English
Published:	2009
Subjects:	Oil palm - Phenols - Case studies
Online Access:	http://psasir.upm.edu.my/id/eprint/5728/1/FSTM_2009_1_abstract.pdf
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id	my-upm-ir.5728
record_format	uketd_dc
spelling	my-upm-ir.57282013-05-27T07:24:44Z Characterization and Antioxidant Activity of Phenolic Extracts from Oil Palm (Elaeis Guineensis) Fruits 2009 Neo, Yun Ping The extracted oil palm fruit phenolics were analysed using spectrophotometry methods to obtain information on the different types of oil palm phenolics and their antioxidative activities. Different methods were used to extract soluble free (SFP), insoluble-bound (ISBP) and esterified (EFP) phenolics for a better understanding of the types of phenolics present. TPC, TFC, ODPI and DPPH of oil palm phenolics were also monitored to investigate the possible relationships between these variables and the degree of maturity/ripeness of the oil palm fruit from 16 to 24 weeks. The antioxidant activities of oil palm phenolic extracts were analysed using different antioxidant assays, namely the 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assay, 2,2-diphenyl-2-picrylhydrazyl (DPPH) assay, the ferric-reducing ability (FRAP) assay, β-carotene bleaching assay (BCB) and the oxidative stability index (OSI). Results showed that oil palm phenolic extracts contained high antioxidant activities in the order of ISBP > EFP > SFP. Eight different phenolic acids were identified and quantified using a simple reversed-phase high performance liquid chromatography (HPLC) with a diode array detector (DAD) and liquid chromatography/ tandem mass spectrometry (LC/MS/MS). Gallic, protocatechuic, p-hydroxybenzoic, vanillic, caffeic, syringic, p-coumaric and ferulic acids were detected in oil palm phenolic extracts. Ferulic, p-hydroxybenzoic and p-coumaric acid were the dominant phenolic acids found in oil palm fruit extracts and ranged from 55 - 376 μg/g of DW. The results suggested the potent antioxidant activities of oil palm phenolic extracts and the presence of phenolic acids in palm fruits. Oil palm - Phenols - Case studies 2009 Thesis http://psasir.upm.edu.my/id/eprint/5728/ http://psasir.upm.edu.my/id/eprint/5728/1/FSTM_2009_1_abstract.pdf application/pdf en public masters Universiti Putra Malaysia Oil palm - Phenols - Case studies Food Science and Technology English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic	Oil palm - Phenols - Case studies
spellingShingle	Oil palm - Phenols - Case studies Neo, Yun Ping Characterization and Antioxidant Activity of Phenolic Extracts from Oil Palm (Elaeis Guineensis) Fruits
description	The extracted oil palm fruit phenolics were analysed using spectrophotometry methods to obtain information on the different types of oil palm phenolics and their antioxidative activities. Different methods were used to extract soluble free (SFP), insoluble-bound (ISBP) and esterified (EFP) phenolics for a better understanding of the types of phenolics present. TPC, TFC, ODPI and DPPH of oil palm phenolics were also monitored to investigate the possible relationships between these variables and the degree of maturity/ripeness of the oil palm fruit from 16 to 24 weeks. The antioxidant activities of oil palm phenolic extracts were analysed using different antioxidant assays, namely the 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assay, 2,2-diphenyl-2-picrylhydrazyl (DPPH) assay, the ferric-reducing ability (FRAP) assay, β-carotene bleaching assay (BCB) and the oxidative stability index (OSI). Results showed that oil palm phenolic extracts contained high antioxidant activities in the order of ISBP > EFP > SFP. Eight different phenolic acids were identified and quantified using a simple reversed-phase high performance liquid chromatography (HPLC) with a diode array detector (DAD) and liquid chromatography/ tandem mass spectrometry (LC/MS/MS). Gallic, protocatechuic, p-hydroxybenzoic, vanillic, caffeic, syringic, p-coumaric and ferulic acids were detected in oil palm phenolic extracts. Ferulic, p-hydroxybenzoic and p-coumaric acid were the dominant phenolic acids found in oil palm fruit extracts and ranged from 55 - 376 μg/g of DW. The results suggested the potent antioxidant activities of oil palm phenolic extracts and the presence of phenolic acids in palm fruits.
format	Thesis
qualification_level	Master's degree
author	Neo, Yun Ping
author_facet	Neo, Yun Ping
author_sort	Neo, Yun Ping
title	Characterization and Antioxidant Activity of Phenolic Extracts from Oil Palm (Elaeis Guineensis) Fruits
title_short	Characterization and Antioxidant Activity of Phenolic Extracts from Oil Palm (Elaeis Guineensis) Fruits
title_full	Characterization and Antioxidant Activity of Phenolic Extracts from Oil Palm (Elaeis Guineensis) Fruits
title_fullStr	Characterization and Antioxidant Activity of Phenolic Extracts from Oil Palm (Elaeis Guineensis) Fruits
title_full_unstemmed	Characterization and Antioxidant Activity of Phenolic Extracts from Oil Palm (Elaeis Guineensis) Fruits
title_sort	characterization and antioxidant activity of phenolic extracts from oil palm (elaeis guineensis) fruits
granting_institution	Universiti Putra Malaysia
granting_department	Food Science and Technology
publishDate	2009
url	http://psasir.upm.edu.my/id/eprint/5728/1/FSTM_2009_1_abstract.pdf
_version_	1747810472917204992

Characterization and Antioxidant Activity of Phenolic Extracts from Oil Palm (Elaeis Guineensis) Fruits

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