Effects of Bifidobacterium Pseudocatenulatum G4 on Clostridium Scindens and Clostridium Hiranonis

The purpose of this study was to investigate the deoxycholic acid (DCA) reduction by Bifidobacterium pseudocatenulatum G4 at in vitro conditions with the emphasis of its bile salt deconjugation ability and antagonistic activity against 7α-dehydroxylating bacteria. B. pseudocatenulatum G4 showed anta...

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Main Author:	Rasti, Babak
Format:	Thesis
Language:	English English
Published:	2009
Subjects:	Bifidobacterium - Clostridium - Case studies
Online Access:	http://psasir.upm.edu.my/id/eprint/5736/1/FSTM_2009_6_abstract.pdf
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id	my-upm-ir.5736
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spelling	my-upm-ir.57362013-05-27T07:24:47Z Effects of Bifidobacterium Pseudocatenulatum G4 on Clostridium Scindens and Clostridium Hiranonis 2009 Rasti, Babak The purpose of this study was to investigate the deoxycholic acid (DCA) reduction by Bifidobacterium pseudocatenulatum G4 at in vitro conditions with the emphasis of its bile salt deconjugation ability and antagonistic activity against 7α-dehydroxylating bacteria. B. pseudocatenulatum G4 showed antagonistic activity against Clostridium scindens and C. hiranonis at colonic pH (5.7, 6.2 and 6.8), with highest activity (4mm inhibition zone) at pH 5.7 against C. hiranonis. Growth rates of B. pseudocatenulatum G4 (time required for the initial absorbance at zero time to increase by 0.3 units), decreased in the presence of 2.0% oxgall compared to absence of oxgall. Effect of B. pseudocatenulatum G4 on pH reduction was measured; B. pseudocatenulatum G4 reduced pH from 6.8 to 3.9 in TPY broth supplemented with 0.1% and pH 3.74 in TPY broth without oxgall (control), compared to pH 5.28 in TPY broth supplemented with 2.0% oxgall. This results showed oxgall exert inhibitory effect on pH reduction by B. pseudocatenulatum G4. Bile salt hydrolase (BSH) activity, which is the measurement of enzyme activity responsible for bile salt deconjugation, was quantified by high pressure liquid chromatography (HPLC) assay. B. pseudocatenulatum G4 gave deconjugation rate (disappearance of conjugated bile acid) in TPY broth supplemented with 0.25 mM (the highest concentration in colon) and 5.0 mM (the highest concentration in small intestine) of all 6 different conjugated bile acids (TCA, GCA, TCDCA, GCDCA, TDCA, and GDCA). Generally B. pseudocatenulatum G4 deconjugated glycoconjugated bile acids in higher amount compare to tauroconjugates. The percentage of deconjugation activity was higher in TPY medium supplemented with 0.25mM bile acids compared to TPY broth with 5.0mM bile acids. On other hand, pH 6.2 was observed as the optimum pH for BSH activity. Full factorial statistical design was used to evaluate the effects of three factors (pH, B. pseudocatenulatum G4 concentrations and Clostridiums) with different levels and interactions between them, on DCA production. Statistical analysis for results of the in vitro experiment showed that total DCA concentration produced by C. scindens and C. hiranonis in the presence of B. pseudocatenulatum G4 and low pH, were lower than the control group with highest pH and in the absence of B. pseudocatenulatum G4. Bifidobacterium - Clostridium - Case studies 2009 Thesis http://psasir.upm.edu.my/id/eprint/5736/ http://psasir.upm.edu.my/id/eprint/5736/1/FSTM_2009_6_abstract.pdf application/pdf en public masters Universiti Putra Malaysia Bifidobacterium - Clostridium - Case studies Food Science and Technology English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic	Bifidobacterium - Clostridium - Case studies
spellingShingle	Bifidobacterium - Clostridium - Case studies Rasti, Babak Effects of Bifidobacterium Pseudocatenulatum G4 on Clostridium Scindens and Clostridium Hiranonis
description	The purpose of this study was to investigate the deoxycholic acid (DCA) reduction by Bifidobacterium pseudocatenulatum G4 at in vitro conditions with the emphasis of its bile salt deconjugation ability and antagonistic activity against 7α-dehydroxylating bacteria. B. pseudocatenulatum G4 showed antagonistic activity against Clostridium scindens and C. hiranonis at colonic pH (5.7, 6.2 and 6.8), with highest activity (4mm inhibition zone) at pH 5.7 against C. hiranonis. Growth rates of B. pseudocatenulatum G4 (time required for the initial absorbance at zero time to increase by 0.3 units), decreased in the presence of 2.0% oxgall compared to absence of oxgall. Effect of B. pseudocatenulatum G4 on pH reduction was measured; B. pseudocatenulatum G4 reduced pH from 6.8 to 3.9 in TPY broth supplemented with 0.1% and pH 3.74 in TPY broth without oxgall (control), compared to pH 5.28 in TPY broth supplemented with 2.0% oxgall. This results showed oxgall exert inhibitory effect on pH reduction by B. pseudocatenulatum G4. Bile salt hydrolase (BSH) activity, which is the measurement of enzyme activity responsible for bile salt deconjugation, was quantified by high pressure liquid chromatography (HPLC) assay. B. pseudocatenulatum G4 gave deconjugation rate (disappearance of conjugated bile acid) in TPY broth supplemented with 0.25 mM (the highest concentration in colon) and 5.0 mM (the highest concentration in small intestine) of all 6 different conjugated bile acids (TCA, GCA, TCDCA, GCDCA, TDCA, and GDCA). Generally B. pseudocatenulatum G4 deconjugated glycoconjugated bile acids in higher amount compare to tauroconjugates. The percentage of deconjugation activity was higher in TPY medium supplemented with 0.25mM bile acids compared to TPY broth with 5.0mM bile acids. On other hand, pH 6.2 was observed as the optimum pH for BSH activity. Full factorial statistical design was used to evaluate the effects of three factors (pH, B. pseudocatenulatum G4 concentrations and Clostridiums) with different levels and interactions between them, on DCA production. Statistical analysis for results of the in vitro experiment showed that total DCA concentration produced by C. scindens and C. hiranonis in the presence of B. pseudocatenulatum G4 and low pH, were lower than the control group with highest pH and in the absence of B. pseudocatenulatum G4.
format	Thesis
qualification_level	Master's degree
author	Rasti, Babak
author_facet	Rasti, Babak
author_sort	Rasti, Babak
title	Effects of Bifidobacterium Pseudocatenulatum G4 on Clostridium Scindens and Clostridium Hiranonis
title_short	Effects of Bifidobacterium Pseudocatenulatum G4 on Clostridium Scindens and Clostridium Hiranonis
title_full	Effects of Bifidobacterium Pseudocatenulatum G4 on Clostridium Scindens and Clostridium Hiranonis
title_fullStr	Effects of Bifidobacterium Pseudocatenulatum G4 on Clostridium Scindens and Clostridium Hiranonis
title_full_unstemmed	Effects of Bifidobacterium Pseudocatenulatum G4 on Clostridium Scindens and Clostridium Hiranonis
title_sort	effects of bifidobacterium pseudocatenulatum g4 on clostridium scindens and clostridium hiranonis
granting_institution	Universiti Putra Malaysia
granting_department	Food Science and Technology
publishDate	2009
url	http://psasir.upm.edu.my/id/eprint/5736/1/FSTM_2009_6_abstract.pdf
_version_	1747810474854973440

Effects of Bifidobacterium Pseudocatenulatum G4 on Clostridium Scindens and Clostridium Hiranonis

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