Construction of RFLP and AFLP Genetic Linkage Maps For Oil Palm (Elaeis Guineensis Jacq.) Using a Deli Dura X Yangambi Pisifera Cross

Conventional oil palm improvement using traditional breeding is a slow and expensive process. If markers linked to a useful trait, such as yield, shell thickness and embryogenesis rate, can be identified, marker-assisted selection (MAS) can be carried out, which can reduce the time taken for convent...

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Main Author:	Chua, Kia Ling
Format:	Thesis
Language:	English English
Published:	2006
Subjects:	Oil palm. Genetic recombination - Research.
Online Access:	http://psasir.upm.edu.my/id/eprint/582/1/600410_fs_2006_28_abstrak_je__dh_pdf_.pdf
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spelling	my-upm-ir.5822013-05-27T06:49:29Z Construction of RFLP and AFLP Genetic Linkage Maps For Oil Palm (Elaeis Guineensis Jacq.) Using a Deli Dura X Yangambi Pisifera Cross 2006-02 Chua, Kia Ling Conventional oil palm improvement using traditional breeding is a slow and expensive process. If markers linked to a useful trait, such as yield, shell thickness and embryogenesis rate, can be identified, marker-assisted selection (MAS) can be carried out, which can reduce the time taken for conventional breeding. Generating a linkage map is the first step towards marker-assisted selection. In this study, two oil palm maps were generated based on 87 F1 progeny of a controlled cross (Deli dura x Yangambi pisifera). A total of 106 RFLP markers and 171 AFLP markers were identified and scored. Of the 277 markers scored, 28 markers (10.1%) were deviated from expected Mendelian ratio (p<0.05). Pseudo-testcross strategy was used to generate two parental maps. The dura map consisted of 18 linkage groups and covered a total map distance of 584.1cM. The pisifera map resolved into 19 linkage groups and covered a total map distance of 1099.3cM. Of all the markers analyzed, 16.9% of the dura markers and 25.1% of the pisifera markers remained unlinked. RFLP marker although difficult to develop, proved very useful because only a small fraction is deviated from the expected Mendelian ratio. Furthermore, about 80% of the RFLP markers can be mapped on both parental maps. More markers will be needed to reduce the number of linkage groups of both parental maps to the haploid chromosome number of oil palm (n=16). Five homologous regions between the dura and the pisifera maps were identified by comparing the co-dominant RFLP markers. The orders of the homologous markers were conserved and the overall distances were nearly the same in both varieties, although a small difference was observed in one homologous region on linkage group D3 and P5. This difference might be due to unequal recombinations that occurred at that particular region. Oil palm. Genetic recombination - Research. 2006-02 Thesis http://psasir.upm.edu.my/id/eprint/582/ http://psasir.upm.edu.my/id/eprint/582/1/600410_fs_2006_28_abstrak_je__dh_pdf_.pdf application/pdf en public masters Universiti Putra Malaysia Oil palm. Genetic recombination - Research. Faculty of Science English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic	Oil palm. Genetic recombination - Research.
spellingShingle	Oil palm. Genetic recombination - Research. Chua, Kia Ling Construction of RFLP and AFLP Genetic Linkage Maps For Oil Palm (Elaeis Guineensis Jacq.) Using a Deli Dura X Yangambi Pisifera Cross
description	Conventional oil palm improvement using traditional breeding is a slow and expensive process. If markers linked to a useful trait, such as yield, shell thickness and embryogenesis rate, can be identified, marker-assisted selection (MAS) can be carried out, which can reduce the time taken for conventional breeding. Generating a linkage map is the first step towards marker-assisted selection. In this study, two oil palm maps were generated based on 87 F1 progeny of a controlled cross (Deli dura x Yangambi pisifera). A total of 106 RFLP markers and 171 AFLP markers were identified and scored. Of the 277 markers scored, 28 markers (10.1%) were deviated from expected Mendelian ratio (p<0.05). Pseudo-testcross strategy was used to generate two parental maps. The dura map consisted of 18 linkage groups and covered a total map distance of 584.1cM. The pisifera map resolved into 19 linkage groups and covered a total map distance of 1099.3cM. Of all the markers analyzed, 16.9% of the dura markers and 25.1% of the pisifera markers remained unlinked. RFLP marker although difficult to develop, proved very useful because only a small fraction is deviated from the expected Mendelian ratio. Furthermore, about 80% of the RFLP markers can be mapped on both parental maps. More markers will be needed to reduce the number of linkage groups of both parental maps to the haploid chromosome number of oil palm (n=16). Five homologous regions between the dura and the pisifera maps were identified by comparing the co-dominant RFLP markers. The orders of the homologous markers were conserved and the overall distances were nearly the same in both varieties, although a small difference was observed in one homologous region on linkage group D3 and P5. This difference might be due to unequal recombinations that occurred at that particular region.
format	Thesis
qualification_level	Master's degree
author	Chua, Kia Ling
author_facet	Chua, Kia Ling
author_sort	Chua, Kia Ling
title	Construction of RFLP and AFLP Genetic Linkage Maps For Oil Palm (Elaeis Guineensis Jacq.) Using a Deli Dura X Yangambi Pisifera Cross
title_short	Construction of RFLP and AFLP Genetic Linkage Maps For Oil Palm (Elaeis Guineensis Jacq.) Using a Deli Dura X Yangambi Pisifera Cross
title_full	Construction of RFLP and AFLP Genetic Linkage Maps For Oil Palm (Elaeis Guineensis Jacq.) Using a Deli Dura X Yangambi Pisifera Cross
title_fullStr	Construction of RFLP and AFLP Genetic Linkage Maps For Oil Palm (Elaeis Guineensis Jacq.) Using a Deli Dura X Yangambi Pisifera Cross
title_full_unstemmed	Construction of RFLP and AFLP Genetic Linkage Maps For Oil Palm (Elaeis Guineensis Jacq.) Using a Deli Dura X Yangambi Pisifera Cross
title_sort	construction of rflp and aflp genetic linkage maps for oil palm (elaeis guineensis jacq.) using a deli dura x yangambi pisifera cross
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Science
publishDate	2006
url	http://psasir.upm.edu.my/id/eprint/582/1/600410_fs_2006_28_abstrak_je__dh_pdf_.pdf
_version_	1747810254295400448

Construction of RFLP and AFLP Genetic Linkage Maps For Oil Palm (Elaeis Guineensis Jacq.) Using a Deli Dura X Yangambi Pisifera Cross

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