Development of Bacillus strain B12 and B45 as putative probiotics to improve growth and survival of Epinephelus fuscoguttatus (Forsskal, 1775) juvenile challenged with vibrio alginolyticus

Occurrences of bacterial diseases and the overexploitation to overcome it have resulted to the emergence of antibiotic resistant microorganisms. In relation to this matter,experiments were performed in this study by identifying putative probionts, screening of putative probionts against selected pat...

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Main Author:	Abd Razak, Nabilah Fatin
Format:	Thesis
Language:	English
Published:	2015
Subjects:	Bacillus subtilis - Development - Health aspects Probiotics - Therapeutic use Epinephelus guttatus - Growth
Online Access:	http://psasir.upm.edu.my/id/eprint/58670/1/FP%202015%2058IR.pdf
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id	my-upm-ir.58670
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spelling	my-upm-ir.586702018-01-26T09:39:13Z Development of Bacillus strain B12 and B45 as putative probiotics to improve growth and survival of Epinephelus fuscoguttatus (Forsskal, 1775) juvenile challenged with vibrio alginolyticus 2015-11 Abd Razak, Nabilah Fatin Occurrences of bacterial diseases and the overexploitation to overcome it have resulted to the emergence of antibiotic resistant microorganisms. In relation to this matter,experiments were performed in this study by identifying putative probionts, screening of putative probionts against selected pathogenic strains based on their in vitro antagonism activities, in vivo safety test, feeding trial of E. fuscoguttatus juveniles and challenge with V. alginolyticus. Gram’s method had classified 44 bacterial strains as rod, gram positive cells while biochemical test (BBL CrystalTM identification system) had identified 44 bacterial strains to species level. From 44 bacterial strains, 22 were non-haemolytic isolates. Agar well diffusion assay have demonstrated two bacilli strains; B12 and B45 elucidating intermediate (++; 11 mm – 15 mm) to highest level (+++; >16 mm) of inhibition zones against Vibrio alginolyticus, V. parahaemolyticus,V. harveyi and Aeromonas hydrophila. The broth culture assay in this study observed a complete inhibitory activity of Bacillusstrain B12 towards V. alginolyticus at preincubation cell density of 105 and 107 cfu/mL while Bacillus strain B45 have displayed no inhibitory activity. 16S rRNA gene sequencing have identified Bacillus strain B12 as Bacillus amyloliquafaciens (94% similarity) and Bacillusstrain B45 as Bacillus subtilis (95% similarity). Pathogenicity test of Bacillus strain B12 and B45 by intraperitoneal (IP) injection have revealed no sort of abnormalities or mortalities on E. fuscoguttatus juveniles. Supplementation of Bacillus strain B12 into feed of E.fuscoguttatus juveniles showed an efficient feed conversion ratio (FCR) of 1.17 + 0.07, increased specific growth rate (2.66 + 0.23) and survival (98 + 1.73%). Application of Bacillus strain B12 into daily feed had significantly increased survival (P<0.05) to 71.65% when E. fuscoguttatus juveniles were challenged with V. alginolyticus. To summarize, experimental findings in this study showed that Bacillus strain B12 at cell density of 107 cfu/mL have the potential to be used as feed additive to improve growth and survival of E. fuscoguttatus juveniles. Bacillus subtilis - Development - Health aspects Probiotics - Therapeutic use Epinephelus guttatus - Growth 2015-11 Thesis http://psasir.upm.edu.my/id/eprint/58670/ http://psasir.upm.edu.my/id/eprint/58670/1/FP%202015%2058IR.pdf application/pdf en public masters Universiti Putra Malaysia Bacillus subtilis - Development - Health aspects Probiotics - Therapeutic use Epinephelus guttatus - Growth
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
topic	Bacillus subtilis - Development - Health aspects Probiotics - Therapeutic use Epinephelus guttatus - Growth
spellingShingle	Bacillus subtilis - Development - Health aspects Probiotics - Therapeutic use Epinephelus guttatus - Growth Abd Razak, Nabilah Fatin Development of Bacillus strain B12 and B45 as putative probiotics to improve growth and survival of Epinephelus fuscoguttatus (Forsskal, 1775) juvenile challenged with vibrio alginolyticus
description	Occurrences of bacterial diseases and the overexploitation to overcome it have resulted to the emergence of antibiotic resistant microorganisms. In relation to this matter,experiments were performed in this study by identifying putative probionts, screening of putative probionts against selected pathogenic strains based on their in vitro antagonism activities, in vivo safety test, feeding trial of E. fuscoguttatus juveniles and challenge with V. alginolyticus. Gram’s method had classified 44 bacterial strains as rod, gram positive cells while biochemical test (BBL CrystalTM identification system) had identified 44 bacterial strains to species level. From 44 bacterial strains, 22 were non-haemolytic isolates. Agar well diffusion assay have demonstrated two bacilli strains; B12 and B45 elucidating intermediate (++; 11 mm – 15 mm) to highest level (+++; >16 mm) of inhibition zones against Vibrio alginolyticus, V. parahaemolyticus,V. harveyi and Aeromonas hydrophila. The broth culture assay in this study observed a complete inhibitory activity of Bacillusstrain B12 towards V. alginolyticus at preincubation cell density of 105 and 107 cfu/mL while Bacillus strain B45 have displayed no inhibitory activity. 16S rRNA gene sequencing have identified Bacillus strain B12 as Bacillus amyloliquafaciens (94% similarity) and Bacillusstrain B45 as Bacillus subtilis (95% similarity). Pathogenicity test of Bacillus strain B12 and B45 by intraperitoneal (IP) injection have revealed no sort of abnormalities or mortalities on E. fuscoguttatus juveniles. Supplementation of Bacillus strain B12 into feed of E.fuscoguttatus juveniles showed an efficient feed conversion ratio (FCR) of 1.17 + 0.07, increased specific growth rate (2.66 + 0.23) and survival (98 + 1.73%). Application of Bacillus strain B12 into daily feed had significantly increased survival (P<0.05) to 71.65% when E. fuscoguttatus juveniles were challenged with V. alginolyticus. To summarize, experimental findings in this study showed that Bacillus strain B12 at cell density of 107 cfu/mL have the potential to be used as feed additive to improve growth and survival of E. fuscoguttatus juveniles.
format	Thesis
qualification_level	Master's degree
author	Abd Razak, Nabilah Fatin
author_facet	Abd Razak, Nabilah Fatin
author_sort	Abd Razak, Nabilah Fatin
title	Development of Bacillus strain B12 and B45 as putative probiotics to improve growth and survival of Epinephelus fuscoguttatus (Forsskal, 1775) juvenile challenged with vibrio alginolyticus
title_short	Development of Bacillus strain B12 and B45 as putative probiotics to improve growth and survival of Epinephelus fuscoguttatus (Forsskal, 1775) juvenile challenged with vibrio alginolyticus
title_full	Development of Bacillus strain B12 and B45 as putative probiotics to improve growth and survival of Epinephelus fuscoguttatus (Forsskal, 1775) juvenile challenged with vibrio alginolyticus
title_fullStr	Development of Bacillus strain B12 and B45 as putative probiotics to improve growth and survival of Epinephelus fuscoguttatus (Forsskal, 1775) juvenile challenged with vibrio alginolyticus
title_full_unstemmed	Development of Bacillus strain B12 and B45 as putative probiotics to improve growth and survival of Epinephelus fuscoguttatus (Forsskal, 1775) juvenile challenged with vibrio alginolyticus
title_sort	development of bacillus strain b12 and b45 as putative probiotics to improve growth and survival of epinephelus fuscoguttatus (forsskal, 1775) juvenile challenged with vibrio alginolyticus
granting_institution	Universiti Putra Malaysia
publishDate	2015
url	http://psasir.upm.edu.my/id/eprint/58670/1/FP%202015%2058IR.pdf
_version_	1747812222632984576

Development of Bacillus strain B12 and B45 as putative probiotics to improve growth and survival of Epinephelus fuscoguttatus (Forsskal, 1775) juvenile challenged with vibrio alginolyticus

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