Delivery Systems of Trichoderma Inoculants for the Control of Rhizoctonia Diseases in Brassica Rapa

Trichoderma spp were tested as a biocontrol agent against Rhizoctonia solani Kuhn on Brassica rapa. R. solani was isolated from infected vegetables and confirmed the pathogenic to three cultivars of Brassica causing pre-and post emergence damping-off. Confrontation assay, based on the inhibition...

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Main Author:	Mohamed Daghman, Ibrahim
Format:	Thesis
Language:	English English
Published:	2005
Subjects:	Trichoderma Rhizoctonia solani Biological pest control agents
Online Access:	http://psasir.upm.edu.my/id/eprint/59/1/1000548931_t_fp_2005_37.pdf
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spelling	my-upm-ir.592013-05-27T06:45:22Z Delivery Systems of Trichoderma Inoculants for the Control of Rhizoctonia Diseases in Brassica Rapa 2005-01 Mohamed Daghman, Ibrahim Trichoderma spp were tested as a biocontrol agent against Rhizoctonia solani Kuhn on Brassica rapa. R. solani was isolated from infected vegetables and confirmed the pathogenic to three cultivars of Brassica causing pre-and post emergence damping-off. Confrontation assay, based on the inhibition of mycelial growth and colony overgrowth, showed different degrees of antagonism by Trichoderma isolates to R. solani through competition and mycoparasitism. Two primers (OPC-11 and OPC-15), and RAPD-PCR analysis were used to establish the variability between the nine Trichoderma isolates. The results of which were used to construct a Dendogram. OPC-11 generated 19 RAPD fragments ranging from 200 bp to 3000 bp and OPC-15 generated 18 RAPD fragments ranging from 300 bp to 4000 bp. They successfully grouped the Trichoderma isolates into two main clusters as species aggregates viz. T. harzianum and T. virens. The DNA polymorphism confirmed the identification based on cultural and morphological characteristic. Two of the isolates T. harzanium (UPM40) and T. virens (TV3) were found to establish well on and colonize the roots and rhizospheres of B. rapa. The mixture substrate rice flour and molasses (1:4 RF: ML w/v), incubated three days under shaked culture (100 rpm), was the best for the inoculum production of both Trichoderma isolates. It produced the most conidia (30 x 106 and 22.13 x 106 conidia / g dry mycelium) and mycelium (42.65 mg and 43.97 mg dry mycelium) for UPM40 and TV3, respectively, after only three days incubation. The biomass production was highest at 30 oC incubation temperature for both isolates. Further, desiccation for four days at this temperature still produced good spore viability. UPM40 incorporated on oil palm trunk and chicken dung (OPTCD) and stored at 28 oC and 20 % MC had a significantly longer (P ≤ 0.05) shelf life than other treatments. The shelf lives of the liquid antagonist preparations (UPM40 and TV3) on two food bases and carriers (distilled water and ¼ strength 1:4 RF: ML medium) kept at 10 oC and 28 oC were also evaluated. UPM40 incorporated in the ¼ strength medium and kept at 28 oC had a significantly longer shelf-life (P ≤ 0.05). The dry and liquid formulations of UPM40 could reduce the population of viable R. solani colonies in the infested soil. Both the applications were as effective as the fungicide (Brassicol® 200 mg / L) in protecting the B. rapa against Rhizoctonia pre- and post-emergence damping-off. Bio-coating the seeds of Brassica with similarly reduced damping-off by R. solani Trichoderma Rhizoctonia solani Biological pest control agents 2005-01 Thesis http://psasir.upm.edu.my/id/eprint/59/ http://psasir.upm.edu.my/id/eprint/59/1/1000548931_t_fp_2005_37.pdf application/pdf en public phd doctoral Universiti Putra Malaysia Trichoderma Rhizoctonia solani Biological pest control agents Faculty of Agriculture English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic	Trichoderma Rhizoctonia solani Biological pest control agents
spellingShingle	Trichoderma Rhizoctonia solani Biological pest control agents Mohamed Daghman, Ibrahim Delivery Systems of Trichoderma Inoculants for the Control of Rhizoctonia Diseases in Brassica Rapa
description	Trichoderma spp were tested as a biocontrol agent against Rhizoctonia solani Kuhn on Brassica rapa. R. solani was isolated from infected vegetables and confirmed the pathogenic to three cultivars of Brassica causing pre-and post emergence damping-off. Confrontation assay, based on the inhibition of mycelial growth and colony overgrowth, showed different degrees of antagonism by Trichoderma isolates to R. solani through competition and mycoparasitism. Two primers (OPC-11 and OPC-15), and RAPD-PCR analysis were used to establish the variability between the nine Trichoderma isolates. The results of which were used to construct a Dendogram. OPC-11 generated 19 RAPD fragments ranging from 200 bp to 3000 bp and OPC-15 generated 18 RAPD fragments ranging from 300 bp to 4000 bp. They successfully grouped the Trichoderma isolates into two main clusters as species aggregates viz. T. harzianum and T. virens. The DNA polymorphism confirmed the identification based on cultural and morphological characteristic. Two of the isolates T. harzanium (UPM40) and T. virens (TV3) were found to establish well on and colonize the roots and rhizospheres of B. rapa. The mixture substrate rice flour and molasses (1:4 RF: ML w/v), incubated three days under shaked culture (100 rpm), was the best for the inoculum production of both Trichoderma isolates. It produced the most conidia (30 x 106 and 22.13 x 106 conidia / g dry mycelium) and mycelium (42.65 mg and 43.97 mg dry mycelium) for UPM40 and TV3, respectively, after only three days incubation. The biomass production was highest at 30 oC incubation temperature for both isolates. Further, desiccation for four days at this temperature still produced good spore viability. UPM40 incorporated on oil palm trunk and chicken dung (OPTCD) and stored at 28 oC and 20 % MC had a significantly longer (P ≤ 0.05) shelf life than other treatments. The shelf lives of the liquid antagonist preparations (UPM40 and TV3) on two food bases and carriers (distilled water and ¼ strength 1:4 RF: ML medium) kept at 10 oC and 28 oC were also evaluated. UPM40 incorporated in the ¼ strength medium and kept at 28 oC had a significantly longer shelf-life (P ≤ 0.05). The dry and liquid formulations of UPM40 could reduce the population of viable R. solani colonies in the infested soil. Both the applications were as effective as the fungicide (Brassicol® 200 mg / L) in protecting the B. rapa against Rhizoctonia pre- and post-emergence damping-off. Bio-coating the seeds of Brassica with similarly reduced damping-off by R. solani
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Mohamed Daghman, Ibrahim
author_facet	Mohamed Daghman, Ibrahim
author_sort	Mohamed Daghman, Ibrahim
title	Delivery Systems of Trichoderma Inoculants for the Control of Rhizoctonia Diseases in Brassica Rapa
title_short	Delivery Systems of Trichoderma Inoculants for the Control of Rhizoctonia Diseases in Brassica Rapa
title_full	Delivery Systems of Trichoderma Inoculants for the Control of Rhizoctonia Diseases in Brassica Rapa
title_fullStr	Delivery Systems of Trichoderma Inoculants for the Control of Rhizoctonia Diseases in Brassica Rapa
title_full_unstemmed	Delivery Systems of Trichoderma Inoculants for the Control of Rhizoctonia Diseases in Brassica Rapa
title_sort	delivery systems of trichoderma inoculants for the control of rhizoctonia diseases in brassica rapa
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Agriculture
publishDate	2005
url	http://psasir.upm.edu.my/id/eprint/59/1/1000548931_t_fp_2005_37.pdf
_version_	1747810157754056704

Delivery Systems of Trichoderma Inoculants for the Control of Rhizoctonia Diseases in Brassica Rapa

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