The Nucleocapsid Protein Of Newcastle Disease Virus As A Carrier For The Vp1 Polypeptides Of Enterovirus 71

Human enterovirus 71 (EV71) is an important human enterovirus which belongs to the Enterovirus genus of the Picornaviridae family. Large outbreaks of EV71 have been associated with severe central nervous disease (CNS) manifestations including the hand, foot and mouth disease (HFMD). To date, ther...

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Main Author:	Sivasamugham, Lalita Ambigal
Format:	Thesis
Language:	English
Published:	2005
Subjects:	Newcastle disease virus
Online Access:	http://psasir.upm.edu.my/id/eprint/5910/1/FBSB_2005_10%20IR.pdf
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id	my-upm-ir.5910
record_format	uketd_dc
spelling	my-upm-ir.59102022-02-04T02:06:11Z The Nucleocapsid Protein Of Newcastle Disease Virus As A Carrier For The Vp1 Polypeptides Of Enterovirus 71 2005-10 Sivasamugham, Lalita Ambigal Human enterovirus 71 (EV71) is an important human enterovirus which belongs to the Enterovirus genus of the Picornaviridae family. Large outbreaks of EV71 have been associated with severe central nervous disease (CNS) manifestations including the hand, foot and mouth disease (HFMD). To date, there is no effective antiviral drug available to treat the infections. Therefore, the development of an effective vaccine is considered as one of the best choice to prevent the diseases caused by EV71. The VP1 protein which is the most immunogenic capsid protein of EV71, can be used in the development of subunit EV71 vaccines. The complete VP1 protein of EV71 was truncated into six regions and fused to the full length nucleocapsid protein (NPfl) and truncated NP (NPt; which lacks 20% amino acids from its Cterminal end). Western blot analysis using rabbit anti-VP1 serum showed that the N-terminal region of the VP1 protein contained a major antigenic region. Of all the recombinant NP proteins, the ones carrying truncated VP1 protein, VP11-100 were efficiently expressed in Escherichia coli system. Electron microscopic analysis of the purified NPt-VPl-loo revealed that this protein predominantly selfassembled into intact ring-like structure whereas NPfl-VPl-loo showed disrupted ring-like formations. Rabbits immunized with these purified recombinant proteins exhibited a strong immune response against the complete VP1 protein. The antisera of these recombinant proteins also reacted positively with the authentic EV71 when analyzed by an immuno flourescence assay thus suggesting their potential as subunit vaccine candidates against EV71 infections and also as immunological reagents for the detection of anti-EV71 antibodies in serum samples. Newcastle disease virus 2005-10 Thesis http://psasir.upm.edu.my/id/eprint/5910/ http://psasir.upm.edu.my/id/eprint/5910/1/FBSB_2005_10%20IR.pdf text en public masters Universiti Putra Malaysia Newcastle disease virus Faculty of Biotechnology and Biomocular Sciences Yusoff, Khatijah
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
advisor	Yusoff, Khatijah
topic	Newcastle disease virus
spellingShingle	Newcastle disease virus Sivasamugham, Lalita Ambigal The Nucleocapsid Protein Of Newcastle Disease Virus As A Carrier For The Vp1 Polypeptides Of Enterovirus 71
description	Human enterovirus 71 (EV71) is an important human enterovirus which belongs to the Enterovirus genus of the Picornaviridae family. Large outbreaks of EV71 have been associated with severe central nervous disease (CNS) manifestations including the hand, foot and mouth disease (HFMD). To date, there is no effective antiviral drug available to treat the infections. Therefore, the development of an effective vaccine is considered as one of the best choice to prevent the diseases caused by EV71. The VP1 protein which is the most immunogenic capsid protein of EV71, can be used in the development of subunit EV71 vaccines. The complete VP1 protein of EV71 was truncated into six regions and fused to the full length nucleocapsid protein (NPfl) and truncated NP (NPt; which lacks 20% amino acids from its Cterminal end). Western blot analysis using rabbit anti-VP1 serum showed that the N-terminal region of the VP1 protein contained a major antigenic region. Of all the recombinant NP proteins, the ones carrying truncated VP1 protein, VP11-100 were efficiently expressed in Escherichia coli system. Electron microscopic analysis of the purified NPt-VPl-loo revealed that this protein predominantly selfassembled into intact ring-like structure whereas NPfl-VPl-loo showed disrupted ring-like formations. Rabbits immunized with these purified recombinant proteins exhibited a strong immune response against the complete VP1 protein. The antisera of these recombinant proteins also reacted positively with the authentic EV71 when analyzed by an immuno flourescence assay thus suggesting their potential as subunit vaccine candidates against EV71 infections and also as immunological reagents for the detection of anti-EV71 antibodies in serum samples.
format	Thesis
qualification_level	Master's degree
author	Sivasamugham, Lalita Ambigal
author_facet	Sivasamugham, Lalita Ambigal
author_sort	Sivasamugham, Lalita Ambigal
title	The Nucleocapsid Protein Of Newcastle Disease Virus As A Carrier For The Vp1 Polypeptides Of Enterovirus 71
title_short	The Nucleocapsid Protein Of Newcastle Disease Virus As A Carrier For The Vp1 Polypeptides Of Enterovirus 71
title_full	The Nucleocapsid Protein Of Newcastle Disease Virus As A Carrier For The Vp1 Polypeptides Of Enterovirus 71
title_fullStr	The Nucleocapsid Protein Of Newcastle Disease Virus As A Carrier For The Vp1 Polypeptides Of Enterovirus 71
title_full_unstemmed	The Nucleocapsid Protein Of Newcastle Disease Virus As A Carrier For The Vp1 Polypeptides Of Enterovirus 71
title_sort	nucleocapsid protein of newcastle disease virus as a carrier for the vp1 polypeptides of enterovirus 71
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Biotechnology and Biomocular Sciences
publishDate	2005
url	http://psasir.upm.edu.my/id/eprint/5910/1/FBSB_2005_10%20IR.pdf
_version_	1747810507695325184

The Nucleocapsid Protein Of Newcastle Disease Virus As A Carrier For The Vp1 Polypeptides Of Enterovirus 71

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