Cloning and Characterization of the Apoptotic Suppressor Gene P49 from Spodoptera Litura Nucleopolyhedrovirus

Baculoviruses possess two types of genes that can suppress apoptosis (programmed cell death), p35 and inhibitor of apoptosis (iap). The p49 gene of a Malaysian isolate of Spodoptera Iitura nucleopolyhedrovirus (SpltMNPV), a homolougue of baculovirus p35 gene was cloned and characterized. The open...

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Main Author:	Mat Amin, Noriha
Format:	Thesis
Language:	English
Published:	2005
Subjects:	Spodoptera littoralis
Online Access:	http://psasir.upm.edu.my/id/eprint/5970/1/FBSB_2005_35%20IR.pdf
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id	my-upm-ir.5970
record_format	uketd_dc
spelling	my-upm-ir.59702022-02-17T03:29:02Z Cloning and Characterization of the Apoptotic Suppressor Gene P49 from Spodoptera Litura Nucleopolyhedrovirus 2005-09 Mat Amin, Noriha Baculoviruses possess two types of genes that can suppress apoptosis (programmed cell death), p35 and inhibitor of apoptosis (iap). The p49 gene of a Malaysian isolate of Spodoptera Iitura nucleopolyhedrovirus (SpltMNPV), a homolougue of baculovirus p35 gene was cloned and characterized. The open Reading Frame (OW) of SpltMNPV p49 gene was 13 17 bp long and encodes approximately 349 amino acid residues with a predicted molecular weight of 5 1.1 1 kDa. The SpltMNPV p49 gene shared 99% and 87% identity of nucleotide and amino acid sequence respectively to the SpltMNPV (AF325155), SpltMNPV (AF207549) and Spodoptera littoralis nucleopolyhedrovirus (SlNPV) p49 (AJ00675 1) obtained in the GenBank. The SpltMNPV P49 protein showed amino acid identities fiom 25 to 30% with 37 to 47% similarities to the p35 protein of Leucania separata, Spodoptera litura, Rachiplusia ou, Autographa californica and Bombyx mori nucleopolyhedroviruses, respectively. The SpltMNPV P49 protein molecule displays a potential caspase recognition site TVTDG at amino acid positions 94 to 98 in the polypeptide chain. The predicted secondary structure of SpltMNPV P49 protein including the hydrophilic (polar) and hydrophobic (basic) region was found to be similar to other P49 and P35 protein. Spodoptera littoralis 2005-09 Thesis http://psasir.upm.edu.my/id/eprint/5970/ http://psasir.upm.edu.my/id/eprint/5970/1/FBSB_2005_35%20IR.pdf text en public masters Universiti Putra Malaysia Spodoptera littoralis Faculty of Biotechnology and Biomokular Sciences Mustafa, Shuhaimi
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
advisor	Mustafa, Shuhaimi
topic	Spodoptera littoralis
spellingShingle	Spodoptera littoralis Mat Amin, Noriha Cloning and Characterization of the Apoptotic Suppressor Gene P49 from Spodoptera Litura Nucleopolyhedrovirus
description	Baculoviruses possess two types of genes that can suppress apoptosis (programmed cell death), p35 and inhibitor of apoptosis (iap). The p49 gene of a Malaysian isolate of Spodoptera Iitura nucleopolyhedrovirus (SpltMNPV), a homolougue of baculovirus p35 gene was cloned and characterized. The open Reading Frame (OW) of SpltMNPV p49 gene was 13 17 bp long and encodes approximately 349 amino acid residues with a predicted molecular weight of 5 1.1 1 kDa. The SpltMNPV p49 gene shared 99% and 87% identity of nucleotide and amino acid sequence respectively to the SpltMNPV (AF325155), SpltMNPV (AF207549) and Spodoptera littoralis nucleopolyhedrovirus (SlNPV) p49 (AJ00675 1) obtained in the GenBank. The SpltMNPV P49 protein showed amino acid identities fiom 25 to 30% with 37 to 47% similarities to the p35 protein of Leucania separata, Spodoptera litura, Rachiplusia ou, Autographa californica and Bombyx mori nucleopolyhedroviruses, respectively. The SpltMNPV P49 protein molecule displays a potential caspase recognition site TVTDG at amino acid positions 94 to 98 in the polypeptide chain. The predicted secondary structure of SpltMNPV P49 protein including the hydrophilic (polar) and hydrophobic (basic) region was found to be similar to other P49 and P35 protein.
format	Thesis
qualification_level	Master's degree
author	Mat Amin, Noriha
author_facet	Mat Amin, Noriha
author_sort	Mat Amin, Noriha
title	Cloning and Characterization of the Apoptotic Suppressor Gene P49 from Spodoptera Litura Nucleopolyhedrovirus
title_short	Cloning and Characterization of the Apoptotic Suppressor Gene P49 from Spodoptera Litura Nucleopolyhedrovirus
title_full	Cloning and Characterization of the Apoptotic Suppressor Gene P49 from Spodoptera Litura Nucleopolyhedrovirus
title_fullStr	Cloning and Characterization of the Apoptotic Suppressor Gene P49 from Spodoptera Litura Nucleopolyhedrovirus
title_full_unstemmed	Cloning and Characterization of the Apoptotic Suppressor Gene P49 from Spodoptera Litura Nucleopolyhedrovirus
title_sort	cloning and characterization of the apoptotic suppressor gene p49 from spodoptera litura nucleopolyhedrovirus
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Biotechnology and Biomokular Sciences
publishDate	2005
url	http://psasir.upm.edu.my/id/eprint/5970/1/FBSB_2005_35%20IR.pdf
_version_	1747810518238756864

Cloning and Characterization of the Apoptotic Suppressor Gene P49 from Spodoptera Litura Nucleopolyhedrovirus

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