Insulinotropic Properties of Several Malaysian Herbs and Ganoderma Species

Diabetes Mellitus incident is currently one of the major and common diseases in Malaysia and increasing every year. To date, no herbs or drugs are able to completely cure the disease. In this study, insulinotropic properties of Malaysian herbs were investigated using pancreatic P cells BRIN-BD1 1...

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Main Author:	Bastami, Mohd Saufi
Format:	Thesis
Language:	English English
Published:	2006
Subjects:	Herbs - Malaysia - Toxicology Ganoderma - Malaysia - Toxicology
Online Access:	http://psasir.upm.edu.my/id/eprint/5976/1/FBSB_2006_2.pdf
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spelling	my-upm-ir.59762023-01-03T03:23:29Z Insulinotropic Properties of Several Malaysian Herbs and Ganoderma Species 2006-01 Bastami, Mohd Saufi Diabetes Mellitus incident is currently one of the major and common diseases in Malaysia and increasing every year. To date, no herbs or drugs are able to completely cure the disease. In this study, insulinotropic properties of Malaysian herbs were investigated using pancreatic P cells BRIN-BD1 1 . Three out of 13 herbs samples studied were belongs to fungi (Ganoderma sp). The studied showed seven samples are able to induce insulin secretion over 200 % by BRIN-BD11 cell lines. Based on traditional practitioner reports, Gynuraprocumbens was choosed for further investigation on its antihyperglycemic properties. Glucose tolerance test were carried on normal and streptozotocin-induced diabetic rats showed at 1000 mglkgl b.w Gynura procumbens methanolic extract was able to reduce blood glucose level in rats. Toxicity towards BRIN-BD11 cells was analyzed using MTT assay and alkaline comet assay. Toxicity levels at ICS0 was 300 uglml using MTT assay. Furthermore, Alkaline Comet Assay has proved this toxicity is not due to DNA damage in BRIN-BDl 1 cells. Acute toxicity was carried on ICR strain mice for 14 days. Gynuraprocumbens did not show any toxicity at concentration 7 glkg b.w after administered orally. These results suggest Gynura procumbens and other herbs contain active constituents and have potentials for diabetes treatment. Herbs - Malaysia - Toxicology Ganoderma - Malaysia - Toxicology 2006-01 Thesis http://psasir.upm.edu.my/id/eprint/5976/ http://psasir.upm.edu.my/id/eprint/5976/1/FBSB_2006_2.pdf text en public masters Universiti Putra Malaysia Herbs - Malaysia - Toxicology Ganoderma - Malaysia - Toxicology Faculty of Biotechnology and Biomokular Sciences English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic	Herbs - Malaysia - Toxicology Ganoderma - Malaysia - Toxicology
spellingShingle	Herbs - Malaysia - Toxicology Ganoderma - Malaysia - Toxicology Bastami, Mohd Saufi Insulinotropic Properties of Several Malaysian Herbs and Ganoderma Species
description	Diabetes Mellitus incident is currently one of the major and common diseases in Malaysia and increasing every year. To date, no herbs or drugs are able to completely cure the disease. In this study, insulinotropic properties of Malaysian herbs were investigated using pancreatic P cells BRIN-BD1 1 . Three out of 13 herbs samples studied were belongs to fungi (Ganoderma sp). The studied showed seven samples are able to induce insulin secretion over 200 % by BRIN-BD11 cell lines. Based on traditional practitioner reports, Gynuraprocumbens was choosed for further investigation on its antihyperglycemic properties. Glucose tolerance test were carried on normal and streptozotocin-induced diabetic rats showed at 1000 mglkgl b.w Gynura procumbens methanolic extract was able to reduce blood glucose level in rats. Toxicity towards BRIN-BD11 cells was analyzed using MTT assay and alkaline comet assay. Toxicity levels at ICS0 was 300 uglml using MTT assay. Furthermore, Alkaline Comet Assay has proved this toxicity is not due to DNA damage in BRIN-BDl 1 cells. Acute toxicity was carried on ICR strain mice for 14 days. Gynuraprocumbens did not show any toxicity at concentration 7 glkg b.w after administered orally. These results suggest Gynura procumbens and other herbs contain active constituents and have potentials for diabetes treatment.
format	Thesis
qualification_level	Master's degree
author	Bastami, Mohd Saufi
author_facet	Bastami, Mohd Saufi
author_sort	Bastami, Mohd Saufi
title	Insulinotropic Properties of Several Malaysian Herbs and Ganoderma Species
title_short	Insulinotropic Properties of Several Malaysian Herbs and Ganoderma Species
title_full	Insulinotropic Properties of Several Malaysian Herbs and Ganoderma Species
title_fullStr	Insulinotropic Properties of Several Malaysian Herbs and Ganoderma Species
title_full_unstemmed	Insulinotropic Properties of Several Malaysian Herbs and Ganoderma Species
title_sort	insulinotropic properties of several malaysian herbs and ganoderma species
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Biotechnology and Biomokular Sciences
publishDate	2006
url	http://psasir.upm.edu.my/id/eprint/5976/1/FBSB_2006_2.pdf
_version_	1776100241232101376

Insulinotropic Properties of Several Malaysian Herbs and Ganoderma Species

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