Analysis of Flavonoids in Oncidium Taka Flowers at Various Stages of Development

Colour is an important factor in determining the aesthetic value, and hence the economic value of flowers. Thus there is active research and development in chemistry of colour pigments especially in flowers and in the breeding of cultivars for preferred colors. Understanding the chemistry of colo...

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Main Author:	Megat Mohd Tahir, Putri Noor Faizah
Format:	Thesis
Language:	English English
Published:	2006
Subjects:	Flavonoids - Oncidium
Online Access:	http://psasir.upm.edu.my/id/eprint/5995/1/FBSB_2006_24.pdf
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spelling	my-upm-ir.59952023-01-03T07:27:43Z Analysis of Flavonoids in Oncidium Taka Flowers at Various Stages of Development 2006-03 Megat Mohd Tahir, Putri Noor Faizah Colour is an important factor in determining the aesthetic value, and hence the economic value of flowers. Thus there is active research and development in chemistry of colour pigments especially in flowers and in the breeding of cultivars for preferred colors. Understanding the chemistry of colour pigments, especially the changes in their composition and concentration as the flower develops, is essential as a background for any attempt at genetic manipulation of flower colour. It is important to know which pigments are normally present so that specific steps in their synthesis can be targeted. In this study the concentration of flavonoids in the flower of the orchid Oncidium Taka, as it develops fiom the bud stage to the mature flower, was determined. Four stages of flower development were arbitrarily selected, viz. the bud stage, partially open flower, fully opened flower and the mature flower. The flavonoids were extracted using methanol and the extract concentrated to dryness. The separation step was by TLC followed by analysis using HPLC and UV Vis spectrophotometer. The standard compounds for flavonoids were kaempferol, luteolin, naringenin and myricetin. The HPLC analysis result showed that kaempferol, luteolin and myricetin were detected in at the level of 14.54 * 3.86, 1.80 0.27 and 3.04 * 1.51 pglg FW respectively at the bud stage. Throughout the experiment, kaempferol concentration decreased as the flower developed to maturity (0.12 0.03 pg/g FW). The same trend was also observed for myricetin (0.24 * 0.1 1 pglg FW) at the third stage but increased at the fourth stage. Luteolin on the other hand remained consistent at approximately 1.35 * 0.44 pg/g FW throughout the development of the flower fiom the bud to the mature stage. Naringenin could not be detected using HPLC at all stages of flower development. The qualitative analysis using TLC and W Vis spectrophotometer also indicated the presence of kaempferol, luteolin and myricetin in Oncidium Taka petals extract. Naringenin was only detected using TLC technique with solvent A (chloroform:acetic acid:water (90:45:6 vlvlv)) and solvent C (chloroform:methanol(100:20 vlv)). All the TLC plates were fumed with iodine vapour. Flavonoids - Oncidium 2006-03 Thesis http://psasir.upm.edu.my/id/eprint/5995/ http://psasir.upm.edu.my/id/eprint/5995/1/FBSB_2006_24.pdf text en public masters Universiti Putra Malaysia Flavonoids - Oncidium Faculty of Biotechnology and Biomokular Sciences English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic	Flavonoids - Oncidium
spellingShingle	Flavonoids - Oncidium Megat Mohd Tahir, Putri Noor Faizah Analysis of Flavonoids in Oncidium Taka Flowers at Various Stages of Development
description	Colour is an important factor in determining the aesthetic value, and hence the economic value of flowers. Thus there is active research and development in chemistry of colour pigments especially in flowers and in the breeding of cultivars for preferred colors. Understanding the chemistry of colour pigments, especially the changes in their composition and concentration as the flower develops, is essential as a background for any attempt at genetic manipulation of flower colour. It is important to know which pigments are normally present so that specific steps in their synthesis can be targeted. In this study the concentration of flavonoids in the flower of the orchid Oncidium Taka, as it develops fiom the bud stage to the mature flower, was determined. Four stages of flower development were arbitrarily selected, viz. the bud stage, partially open flower, fully opened flower and the mature flower. The flavonoids were extracted using methanol and the extract concentrated to dryness. The separation step was by TLC followed by analysis using HPLC and UV Vis spectrophotometer. The standard compounds for flavonoids were kaempferol, luteolin, naringenin and myricetin. The HPLC analysis result showed that kaempferol, luteolin and myricetin were detected in at the level of 14.54 * 3.86, 1.80 0.27 and 3.04 * 1.51 pglg FW respectively at the bud stage. Throughout the experiment, kaempferol concentration decreased as the flower developed to maturity (0.12 0.03 pg/g FW). The same trend was also observed for myricetin (0.24 * 0.1 1 pglg FW) at the third stage but increased at the fourth stage. Luteolin on the other hand remained consistent at approximately 1.35 * 0.44 pg/g FW throughout the development of the flower fiom the bud to the mature stage. Naringenin could not be detected using HPLC at all stages of flower development. The qualitative analysis using TLC and W Vis spectrophotometer also indicated the presence of kaempferol, luteolin and myricetin in Oncidium Taka petals extract. Naringenin was only detected using TLC technique with solvent A (chloroform:acetic acid:water (90:45:6 vlvlv)) and solvent C (chloroform:methanol(100:20 vlv)). All the TLC plates were fumed with iodine vapour.
format	Thesis
qualification_level	Master's degree
author	Megat Mohd Tahir, Putri Noor Faizah
author_facet	Megat Mohd Tahir, Putri Noor Faizah
author_sort	Megat Mohd Tahir, Putri Noor Faizah
title	Analysis of Flavonoids in Oncidium Taka Flowers at Various Stages of Development
title_short	Analysis of Flavonoids in Oncidium Taka Flowers at Various Stages of Development
title_full	Analysis of Flavonoids in Oncidium Taka Flowers at Various Stages of Development
title_fullStr	Analysis of Flavonoids in Oncidium Taka Flowers at Various Stages of Development
title_full_unstemmed	Analysis of Flavonoids in Oncidium Taka Flowers at Various Stages of Development
title_sort	analysis of flavonoids in oncidium taka flowers at various stages of development
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Biotechnology and Biomokular Sciences
publishDate	2006
url	http://psasir.upm.edu.my/id/eprint/5995/1/FBSB_2006_24.pdf
_version_	1776100244657799168

Analysis of Flavonoids in Oncidium Taka Flowers at Various Stages of Development

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