Development of miniaturized tapered fiber optics diamine oxidase-based sensor for histamine detection

Estimating the level of histamine in fish and fish products is very important because of their implication in fish poisoning in human; hence, ascertaining histamine levels in the afore-mentioned serves as a chemical index for spoilage. Factors such as expensive instrumentation, time consumption, siz...

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Main Author:	Usman, Hamza Boko
Format:	Thesis
Language:	English
Published:	2015
Subjects:	Chemical detectors Optical fiber detectors Design
Online Access:	http://psasir.upm.edu.my/id/eprint/60432/1/FBSB%202015%209IR.pdf
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id	my-upm-ir.60432
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spelling	my-upm-ir.604322018-04-26T03:50:19Z Development of miniaturized tapered fiber optics diamine oxidase-based sensor for histamine detection 2015-05 Usman, Hamza Boko Estimating the level of histamine in fish and fish products is very important because of their implication in fish poisoning in human; hence, ascertaining histamine levels in the afore-mentioned serves as a chemical index for spoilage. Factors such as expensive instrumentation, time consumption, size and weight are some of the problems with conventional analytical methods of detection. Tapered fiber optics-enzyme based sensors are prospective candidates towards resolving these quagmires due to their portability and simplicity vis-à-vis currently available detectors. Taking into cognizance immobilization as the backbone of any biosensor, this work, reports a technique to immobilize an ordered multilayer of diamine oxidase (DAO) by means of chemical cross-linking on the biconical tapered fiber surface step-wisely alternating between chitosan, glutaraldehyde (GA) and the enzyme. The optimum parameters for the fabricated biosensor included 160 mg/ml DAO, 0.5% chitosan, 2.5% GA, pH 7.0,and tapered fiber surface of waist diameter 12 μm and length 20 mm. A spectrophotometric signal resulted from horseradish peroxidase catalyzed reduction of H2O2, a secondary product of the oxidative deamination of histamine monitored at 450 nm in 0.1 M phosphate buffer (pH 7.0 and room temperature). Atomic force microscopy (AFM), scanning electron microscope (SEM) and spectrophotometric technique confirmed the functionality of the biosensor. The biosensor showed a response and recovery time of 14 sec, a linear response range up to 1.5 mM, a good sensitivity of 0.64 mM-1 with detection and quantification limits towards histamine of 0.086 mM (15.8 ppm) and 0.204 mM (37.7 ppm) and a linear response range of 0-1.5 mM. The sensor showed an excellent anti-interferents property towards the common interferents’ agents of <5%, with good recovery performance towards varying concentration of histamine ranging from 95.6 to 103.6% (RSD <5%). It showed operational stability to up to 40 repeated analyses without significant loss of sensitivity. The developed miniaturized biosensor has a good potential for use in quantitative measurement of histamine in seafood. Chemical detectors Optical fiber detectors Design 2015-05 Thesis http://psasir.upm.edu.my/id/eprint/60432/ http://psasir.upm.edu.my/id/eprint/60432/1/FBSB%202015%209IR.pdf text en public masters Universiti Putra Malaysia Chemical detectors Optical fiber detectors Design
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
topic	Chemical detectors Optical fiber detectors Design
spellingShingle	Chemical detectors Optical fiber detectors Design Usman, Hamza Boko Development of miniaturized tapered fiber optics diamine oxidase-based sensor for histamine detection
description	Estimating the level of histamine in fish and fish products is very important because of their implication in fish poisoning in human; hence, ascertaining histamine levels in the afore-mentioned serves as a chemical index for spoilage. Factors such as expensive instrumentation, time consumption, size and weight are some of the problems with conventional analytical methods of detection. Tapered fiber optics-enzyme based sensors are prospective candidates towards resolving these quagmires due to their portability and simplicity vis-à-vis currently available detectors. Taking into cognizance immobilization as the backbone of any biosensor, this work, reports a technique to immobilize an ordered multilayer of diamine oxidase (DAO) by means of chemical cross-linking on the biconical tapered fiber surface step-wisely alternating between chitosan, glutaraldehyde (GA) and the enzyme. The optimum parameters for the fabricated biosensor included 160 mg/ml DAO, 0.5% chitosan, 2.5% GA, pH 7.0,and tapered fiber surface of waist diameter 12 μm and length 20 mm. A spectrophotometric signal resulted from horseradish peroxidase catalyzed reduction of H2O2, a secondary product of the oxidative deamination of histamine monitored at 450 nm in 0.1 M phosphate buffer (pH 7.0 and room temperature). Atomic force microscopy (AFM), scanning electron microscope (SEM) and spectrophotometric technique confirmed the functionality of the biosensor. The biosensor showed a response and recovery time of 14 sec, a linear response range up to 1.5 mM, a good sensitivity of 0.64 mM-1 with detection and quantification limits towards histamine of 0.086 mM (15.8 ppm) and 0.204 mM (37.7 ppm) and a linear response range of 0-1.5 mM. The sensor showed an excellent anti-interferents property towards the common interferents’ agents of <5%, with good recovery performance towards varying concentration of histamine ranging from 95.6 to 103.6% (RSD <5%). It showed operational stability to up to 40 repeated analyses without significant loss of sensitivity. The developed miniaturized biosensor has a good potential for use in quantitative measurement of histamine in seafood.
format	Thesis
qualification_level	Master's degree
author	Usman, Hamza Boko
author_facet	Usman, Hamza Boko
author_sort	Usman, Hamza Boko
title	Development of miniaturized tapered fiber optics diamine oxidase-based sensor for histamine detection
title_short	Development of miniaturized tapered fiber optics diamine oxidase-based sensor for histamine detection
title_full	Development of miniaturized tapered fiber optics diamine oxidase-based sensor for histamine detection
title_fullStr	Development of miniaturized tapered fiber optics diamine oxidase-based sensor for histamine detection
title_full_unstemmed	Development of miniaturized tapered fiber optics diamine oxidase-based sensor for histamine detection
title_sort	development of miniaturized tapered fiber optics diamine oxidase-based sensor for histamine detection
granting_institution	Universiti Putra Malaysia
publishDate	2015
url	http://psasir.upm.edu.my/id/eprint/60432/1/FBSB%202015%209IR.pdf
_version_	1747812271889842176

Development of miniaturized tapered fiber optics diamine oxidase-based sensor for histamine detection

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