Endophytic Microorganisms for Improvement of Banana Vigour and Tolerance to Fusarium Wilt

This study explored the potential of endophytic microorganisms (EMS) isolated from wild bananas as biocontrol agents (BCAs) against Fusarium wilt caused by Fusarium oxysporum f. sp. cubense race 4 (FocR4) in susceptible banana ramets (Berangan cv. Intan). The fungal (Fusarium oxysporum (UPM31 PI...

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Main Author:	Ting, Adeline Su Yien
Format:	Thesis
Language:	English English
Published:	2005
Subjects:	Bananas - Case studies Fusarium wilt of banana - Case studies
Online Access:	http://psasir.upm.edu.my/id/eprint/6170/1/FP_2005_9.pdf
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spelling	my-upm-ir.61702023-10-12T03:22:17Z Endophytic Microorganisms for Improvement of Banana Vigour and Tolerance to Fusarium Wilt 2005-10 Ting, Adeline Su Yien This study explored the potential of endophytic microorganisms (EMS) isolated from wild bananas as biocontrol agents (BCAs) against Fusarium wilt caused by Fusarium oxysporum f. sp. cubense race 4 (FocR4) in susceptible banana ramets (Berangan cv. Intan). The fungal (Fusarium oxysporum (UPM31 PI)) and bacterial endophytes (Serratia marcescens (UPM39B3)) were established as effective BCAs; improving growth and vigour and enhancing tolerance of susceptible ramets to Fusarium wilt. They were antagonistic towards FocR4, with Percentage Inhibition of Radial Growth (PIRG) values of 65% and 63%, respectively. Inhibitory substances were produced in the form of volatile as well as non-volatile substances. The endophytes were also able to colonize the host tissues, including the roots and corms in artificial inoculation under laboratory conditions. The association of F. oxysporum (UPM31 PI) and S. marcescens (UPM39B3) with the host plants resulted in enhanced vegetative growth as shown by the increase in height, pseudostem diameter, root mass and total number of leaves ramet-'. Endophytes acted as elicitors in the production of inducible compounds associated with induced resistance (peroxidase, polyphenoloxidase, phenylalanine ammonia lyase, total soluble phenol and lignin content). The robust growth and occurrence of induced resistance subsequently enhanced tolerance of the ramets to Fusarium wilt based on parameters such as delay in onset of symptoms, lower percentages in disease incidence, disease severity, and epidemic rate. Inoculation with F. oxysporum singly was most effective followed by inoculation with mixture with S. marcescens (UPM31 Pl+UPM39B3). However, the survival and subsequent biocontrol efficacy of F. oxysporum (UPM31 PI) and S. marcescens (UPM39B3) might be vulnerable to the changing soil and environmental conditions. Populations of endophytes were not sustained over time. Therefore, further studies regarding formulation and application frequency and techniques, are essential to maximize the potential of F. oxysporum (UPM31P1) and S. marcescens (UPM39B3) as BCAs against Fusarium wilt of banana. Bananas - Case studies Fusarium wilt of banana - Case studies 2005-10 Thesis http://psasir.upm.edu.my/id/eprint/6170/ http://psasir.upm.edu.my/id/eprint/6170/1/FP_2005_9.pdf text en public doctoral Universiti Putra Malaysia Bananas - Case studies Fusarium wilt of banana - Case studies Faculty Agriculture Meon, Sariah English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
advisor	Meon, Sariah
topic	Bananas - Case studies Fusarium wilt of banana - Case studies
spellingShingle	Bananas - Case studies Fusarium wilt of banana - Case studies Ting, Adeline Su Yien Endophytic Microorganisms for Improvement of Banana Vigour and Tolerance to Fusarium Wilt
description	This study explored the potential of endophytic microorganisms (EMS) isolated from wild bananas as biocontrol agents (BCAs) against Fusarium wilt caused by Fusarium oxysporum f. sp. cubense race 4 (FocR4) in susceptible banana ramets (Berangan cv. Intan). The fungal (Fusarium oxysporum (UPM31 PI)) and bacterial endophytes (Serratia marcescens (UPM39B3)) were established as effective BCAs; improving growth and vigour and enhancing tolerance of susceptible ramets to Fusarium wilt. They were antagonistic towards FocR4, with Percentage Inhibition of Radial Growth (PIRG) values of 65% and 63%, respectively. Inhibitory substances were produced in the form of volatile as well as non-volatile substances. The endophytes were also able to colonize the host tissues, including the roots and corms in artificial inoculation under laboratory conditions. The association of F. oxysporum (UPM31 PI) and S. marcescens (UPM39B3) with the host plants resulted in enhanced vegetative growth as shown by the increase in height, pseudostem diameter, root mass and total number of leaves ramet-'. Endophytes acted as elicitors in the production of inducible compounds associated with induced resistance (peroxidase, polyphenoloxidase, phenylalanine ammonia lyase, total soluble phenol and lignin content). The robust growth and occurrence of induced resistance subsequently enhanced tolerance of the ramets to Fusarium wilt based on parameters such as delay in onset of symptoms, lower percentages in disease incidence, disease severity, and epidemic rate. Inoculation with F. oxysporum singly was most effective followed by inoculation with mixture with S. marcescens (UPM31 Pl+UPM39B3). However, the survival and subsequent biocontrol efficacy of F. oxysporum (UPM31 PI) and S. marcescens (UPM39B3) might be vulnerable to the changing soil and environmental conditions. Populations of endophytes were not sustained over time. Therefore, further studies regarding formulation and application frequency and techniques, are essential to maximize the potential of F. oxysporum (UPM31P1) and S. marcescens (UPM39B3) as BCAs against Fusarium wilt of banana.
format	Thesis
qualification_level	Doctorate
author	Ting, Adeline Su Yien
author_facet	Ting, Adeline Su Yien
author_sort	Ting, Adeline Su Yien
title	Endophytic Microorganisms for Improvement of Banana Vigour and Tolerance to Fusarium Wilt
title_short	Endophytic Microorganisms for Improvement of Banana Vigour and Tolerance to Fusarium Wilt
title_full	Endophytic Microorganisms for Improvement of Banana Vigour and Tolerance to Fusarium Wilt
title_fullStr	Endophytic Microorganisms for Improvement of Banana Vigour and Tolerance to Fusarium Wilt
title_full_unstemmed	Endophytic Microorganisms for Improvement of Banana Vigour and Tolerance to Fusarium Wilt
title_sort	endophytic microorganisms for improvement of banana vigour and tolerance to fusarium wilt
granting_institution	Universiti Putra Malaysia
granting_department	Faculty Agriculture
publishDate	2005
url	http://psasir.upm.edu.my/id/eprint/6170/1/FP_2005_9.pdf
_version_	1783725695676973056

Endophytic Microorganisms for Improvement of Banana Vigour and Tolerance to Fusarium Wilt

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