Protein Profiling of Several Malaysian Freshwater Fish by Two-Dimensional Electrophoresis
A proteornics approach using 2D-PAGE was initiated to investigate protein expression in fish organs especially hepatopancreas, with the aim of providing fish protein expression maps that are seriously lacking in the literature. Two dimensional electrophoresis maps of hepatopancreas from Jelawat (...
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| Format: | Thesis |
| Language: | English English |
| Published: |
2004
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| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/6208/1/FS_2005_7.pdf |
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| Summary: | A proteornics approach using 2D-PAGE was initiated to investigate protein
expression in fish organs especially hepatopancreas, with the aim of providing
fish protein expression maps that are seriously lacking in the literature. Two
dimensional electrophoresis maps of hepatopancreas from Jelawat (Leptobarbus
hoevenii), Catfish (Clarias batrachus), Red tilapia (Oreochromis mossambicus), Patin
(Pangasius pangasius) and Lampam Jawa (Puntius gonionotus); all Malaysian
freshwater fish species were constructed. The soluble protein sample was
analyzed by two dimensional electrophoresis, using immobilized pH gradient
strip (pH 3-10) and 15% gel acrylamide. 200 protein spots from Red Tilapia and
44 spots from Catfish were observed. Compari sons of similarities in terms of
protein spots in the fish species were made using ruler measurements and
superimposing the electropherogram of one fish species with the other to
confirm spots coordinate similarities. The maximum matching found in terms of
pI and molecular weight coordinate is limited to a maximum of three fish
species. Patin-Lampam Jawa-Jelawat and Lampam Jawa-Red tilapia-Jelawat share the most number of spots at 8 spots. At the two-fish comparison level, the
highest number of same spots was found in Red tilapia-Jelawat (33 spots)
followed by Lampam Jawa-Jelawat (25 spots), Lampam Jawa-Red tilapia (20
spots), Patin-Lampam Jawa (17 spots), Patin-Red tilapia, Patin- Jelawat and
Lampam Jawa-Catfish; all with 12 same spots. |
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