Protein Profiling of Several Malaysian Freshwater Fish by Two-Dimensional Electrophoresis

A proteornics approach using 2D-PAGE was initiated to investigate protein expression in fish organs especially hepatopancreas, with the aim of providing fish protein expression maps that are seriously lacking in the literature. Two dimensional electrophoresis maps of hepatopancreas from Jelawat (...

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Main Author:	Masdor, Nor Azlina
Format:	Thesis
Language:	English English
Published:	2004
Subjects:	Proteins - Freshwater fishes - Malaysia - Electrophorensis - Case studies
Online Access:	http://psasir.upm.edu.my/id/eprint/6208/1/FS_2005_7.pdf
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id	my-upm-ir.6208
record_format	uketd_dc
spelling	my-upm-ir.62082023-10-16T02:30:58Z Protein Profiling of Several Malaysian Freshwater Fish by Two-Dimensional Electrophoresis 2004-12 Masdor, Nor Azlina A proteornics approach using 2D-PAGE was initiated to investigate protein expression in fish organs especially hepatopancreas, with the aim of providing fish protein expression maps that are seriously lacking in the literature. Two dimensional electrophoresis maps of hepatopancreas from Jelawat (Leptobarbus hoevenii), Catfish (Clarias batrachus), Red tilapia (Oreochromis mossambicus), Patin (Pangasius pangasius) and Lampam Jawa (Puntius gonionotus); all Malaysian freshwater fish species were constructed. The soluble protein sample was analyzed by two dimensional electrophoresis, using immobilized pH gradient strip (pH 3-10) and 15% gel acrylamide. 200 protein spots from Red Tilapia and 44 spots from Catfish were observed. Compari sons of similarities in terms of protein spots in the fish species were made using ruler measurements and superimposing the electropherogram of one fish species with the other to confirm spots coordinate similarities. The maximum matching found in terms of pI and molecular weight coordinate is limited to a maximum of three fish species. Patin-Lampam Jawa-Jelawat and Lampam Jawa-Red tilapia-Jelawat share the most number of spots at 8 spots. At the two-fish comparison level, the highest number of same spots was found in Red tilapia-Jelawat (33 spots) followed by Lampam Jawa-Jelawat (25 spots), Lampam Jawa-Red tilapia (20 spots), Patin-Lampam Jawa (17 spots), Patin-Red tilapia, Patin- Jelawat and Lampam Jawa-Catfish; all with 12 same spots. Proteins - Freshwater fishes - Malaysia - Electrophorensis - Case studies 2004-12 Thesis http://psasir.upm.edu.my/id/eprint/6208/ http://psasir.upm.edu.my/id/eprint/6208/1/FS_2005_7.pdf text en public masters Universiti Putra Malaysia Proteins - Freshwater fishes - Malaysia - Electrophorensis - Case studies Science Omar, Ismail English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
advisor	Omar, Ismail
topic	Proteins - Freshwater fishes - Malaysia - Electrophorensis - Case studies
spellingShingle	Proteins - Freshwater fishes - Malaysia - Electrophorensis - Case studies Masdor, Nor Azlina Protein Profiling of Several Malaysian Freshwater Fish by Two-Dimensional Electrophoresis
description	A proteornics approach using 2D-PAGE was initiated to investigate protein expression in fish organs especially hepatopancreas, with the aim of providing fish protein expression maps that are seriously lacking in the literature. Two dimensional electrophoresis maps of hepatopancreas from Jelawat (Leptobarbus hoevenii), Catfish (Clarias batrachus), Red tilapia (Oreochromis mossambicus), Patin (Pangasius pangasius) and Lampam Jawa (Puntius gonionotus); all Malaysian freshwater fish species were constructed. The soluble protein sample was analyzed by two dimensional electrophoresis, using immobilized pH gradient strip (pH 3-10) and 15% gel acrylamide. 200 protein spots from Red Tilapia and 44 spots from Catfish were observed. Compari sons of similarities in terms of protein spots in the fish species were made using ruler measurements and superimposing the electropherogram of one fish species with the other to confirm spots coordinate similarities. The maximum matching found in terms of pI and molecular weight coordinate is limited to a maximum of three fish species. Patin-Lampam Jawa-Jelawat and Lampam Jawa-Red tilapia-Jelawat share the most number of spots at 8 spots. At the two-fish comparison level, the highest number of same spots was found in Red tilapia-Jelawat (33 spots) followed by Lampam Jawa-Jelawat (25 spots), Lampam Jawa-Red tilapia (20 spots), Patin-Lampam Jawa (17 spots), Patin-Red tilapia, Patin- Jelawat and Lampam Jawa-Catfish; all with 12 same spots.
format	Thesis
qualification_level	Master's degree
author	Masdor, Nor Azlina
author_facet	Masdor, Nor Azlina
author_sort	Masdor, Nor Azlina
title	Protein Profiling of Several Malaysian Freshwater Fish by Two-Dimensional Electrophoresis
title_short	Protein Profiling of Several Malaysian Freshwater Fish by Two-Dimensional Electrophoresis
title_full	Protein Profiling of Several Malaysian Freshwater Fish by Two-Dimensional Electrophoresis
title_fullStr	Protein Profiling of Several Malaysian Freshwater Fish by Two-Dimensional Electrophoresis
title_full_unstemmed	Protein Profiling of Several Malaysian Freshwater Fish by Two-Dimensional Electrophoresis
title_sort	protein profiling of several malaysian freshwater fish by two-dimensional electrophoresis
granting_institution	Universiti Putra Malaysia
granting_department	Science
publishDate	2004
url	http://psasir.upm.edu.my/id/eprint/6208/1/FS_2005_7.pdf
_version_	1783725702700335104

Protein Profiling of Several Malaysian Freshwater Fish by Two-Dimensional Electrophoresis

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