Isolation and Biological Activity of Naturally Occurring Compounds from Melicope Lunu Ankenda (Gaertn) T-Hartley, Melicope Bonwickii(F-Muell) Tharyley and Tetradium Sambucinum (Bl) Hartley

Isolation and Biological Activity of Naturally Occurring Compounds from Melicope Lunu Ankenda (Gaertn) T-Hartley, Melicope Bonwickii(F-Muell) Tharyley and Tetradium Sambucinum (Bl) Hartley

Detailed investigation on three Rutaceous plants have resulted in the isolation and structural elucidation of a number of compounds. The structures of these compounds were elucidated by using spectroscopic methods such as W (ultraviolet), IR (infia red), MS (mass spectra), NMR (Nuclear Magnetic R...

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Main Author: Komala, Ismiarni
Format: Thesis
Language:English
English
Published: 2005
Subjects:
Isolating mechanisms.
Online Access:http://psasir.upm.edu.my/id/eprint/6248/1/FS_2005_30.pdf
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spelling my-upm-ir.62482023-10-18T08:27:06Z Isolation and Biological Activity of Naturally Occurring Compounds from Melicope Lunu Ankenda (Gaertn) T-Hartley, Melicope Bonwickii(F-Muell) Tharyley and Tetradium Sambucinum (Bl) Hartley 2005-06 Komala, Ismiarni Detailed investigation on three Rutaceous plants have resulted in the isolation and structural elucidation of a number of compounds. The structures of these compounds were elucidated by using spectroscopic methods such as W (ultraviolet), IR (infia red), MS (mass spectra), NMR (Nuclear Magnetic Resonance) and also by comparison with previous reports. The crude extracts and isolated compounds were evaluated for their antioxidant, cytotoxicity and antimicrobial activities using DPPH (1,l -diphenil-2-picrylhydrazyl), MTT ((3-(4,5-dimethylthiazol-2-y1)-2,sdiphenyltetrazolium bromide) and disc diffusion methods, respechvely. The phytochemical study on leaves of Mel~cope lum-ankenda (Gaertn) T. Hartley afforded pO-geranylcoumaric acid (1 53) sesamin (1 Og), melisemine (1 54) and a new compound given tentative structure of 7,7"-digeranyloxy-2",3"-epoxycinnamic anhydride (155). The melisemine (154) showed weak activity as a radical scavenger with an ICso value of 75 pg/mL. Meanwhile the 7,7"-digeranyloxy-2",3"- epoxycinnamic anhydride (155) was found to be active against cervical cancer (Hela) cell line with an ICSOv alue of 34 pg/mL. The investigations on leaves of hfelicope bonwickii (F. Ibluell) T. Hartley have resulted in the isolation of three known furoquinoline alkaloid, 7-(2',3'- epoxypreny1oxy)-4-methoxyfuroquinoline (156), evellerine (157) and kokusaginine (11) together with a new 7-(2'-hydroxy-3'-ch1oroprenyloxy)-4- methoxyfuroquinoline (158) and a known amide compound aurantiamide acetate (1 59). Two compounds 7-(2',3 '-epoxypreny1oxy)-4-methoxyfuroquinoline (1 56) and 7-(2'-hydroxy-3'-cl~1loroprenyloxy)-4-methoxyfuroquinol~(e1 58) were found to be toxic to cervical cancer (Hela) cell line with ICso values of 6.0 and 11.4 pg/mL, respectively. Detailed extraction and separation of bark and leaves of Tetradium sambucinum (BI) Hartley have led to the isolation of decarine (160), rutaecarpine (122) 7- hydroxycoumarin (141) and aurantiamide acetate (159). Decarine (160) was active against the cervical cancer cell lines (Hela) with an ICW value of 14.6 pg/mL, while rutaecarpine (122) showed weak a radical scavenger activity with an ICjo value of 75pg/mL. Isolating mechanisms. 2005-06 Thesis http://psasir.upm.edu.my/id/eprint/6248/ http://psasir.upm.edu.my/id/eprint/6248/1/FS_2005_30.pdf text en public masters Universiti Putra Malaysia Isolating mechanisms. Science Rahmani, Mawardi English
institution Universiti Putra Malaysia
collection PSAS Institutional Repository
language English
English
advisor Rahmani, Mawardi
topic Isolating mechanisms.
spellingShingle Isolating mechanisms.


Komala, Ismiarni
Isolation and Biological Activity of Naturally Occurring Compounds from Melicope Lunu Ankenda (Gaertn) T-Hartley, Melicope Bonwickii(F-Muell) Tharyley and Tetradium Sambucinum (Bl) Hartley
description Detailed investigation on three Rutaceous plants have resulted in the isolation and structural elucidation of a number of compounds. The structures of these compounds were elucidated by using spectroscopic methods such as W (ultraviolet), IR (infia red), MS (mass spectra), NMR (Nuclear Magnetic Resonance) and also by comparison with previous reports. The crude extracts and isolated compounds were evaluated for their antioxidant, cytotoxicity and antimicrobial activities using DPPH (1,l -diphenil-2-picrylhydrazyl), MTT ((3-(4,5-dimethylthiazol-2-y1)-2,sdiphenyltetrazolium bromide) and disc diffusion methods, respechvely. The phytochemical study on leaves of Mel~cope lum-ankenda (Gaertn) T. Hartley afforded pO-geranylcoumaric acid (1 53) sesamin (1 Og), melisemine (1 54) and a new compound given tentative structure of 7,7"-digeranyloxy-2",3"-epoxycinnamic anhydride (155). The melisemine (154) showed weak activity as a radical scavenger with an ICso value of 75 pg/mL. Meanwhile the 7,7"-digeranyloxy-2",3"- epoxycinnamic anhydride (155) was found to be active against cervical cancer (Hela) cell line with an ICSOv alue of 34 pg/mL. The investigations on leaves of hfelicope bonwickii (F. Ibluell) T. Hartley have resulted in the isolation of three known furoquinoline alkaloid, 7-(2',3'- epoxypreny1oxy)-4-methoxyfuroquinoline (156), evellerine (157) and kokusaginine (11) together with a new 7-(2'-hydroxy-3'-ch1oroprenyloxy)-4- methoxyfuroquinoline (158) and a known amide compound aurantiamide acetate (1 59). Two compounds 7-(2',3 '-epoxypreny1oxy)-4-methoxyfuroquinoline (1 56) and 7-(2'-hydroxy-3'-cl~1loroprenyloxy)-4-methoxyfuroquinol~(e1 58) were found to be toxic to cervical cancer (Hela) cell line with ICso values of 6.0 and 11.4 pg/mL, respectively. Detailed extraction and separation of bark and leaves of Tetradium sambucinum (BI) Hartley have led to the isolation of decarine (160), rutaecarpine (122) 7- hydroxycoumarin (141) and aurantiamide acetate (159). Decarine (160) was active against the cervical cancer cell lines (Hela) with an ICW value of 14.6 pg/mL, while rutaecarpine (122) showed weak a radical scavenger activity with an ICjo value of 75pg/mL.
format Thesis
qualification_level Master's degree
author Komala, Ismiarni
author_facet Komala, Ismiarni
author_sort Komala, Ismiarni
title Isolation and Biological Activity of Naturally Occurring Compounds from Melicope Lunu Ankenda (Gaertn) T-Hartley, Melicope Bonwickii(F-Muell) Tharyley and Tetradium Sambucinum (Bl) Hartley
title_short Isolation and Biological Activity of Naturally Occurring Compounds from Melicope Lunu Ankenda (Gaertn) T-Hartley, Melicope Bonwickii(F-Muell) Tharyley and Tetradium Sambucinum (Bl) Hartley
title_full Isolation and Biological Activity of Naturally Occurring Compounds from Melicope Lunu Ankenda (Gaertn) T-Hartley, Melicope Bonwickii(F-Muell) Tharyley and Tetradium Sambucinum (Bl) Hartley
title_fullStr Isolation and Biological Activity of Naturally Occurring Compounds from Melicope Lunu Ankenda (Gaertn) T-Hartley, Melicope Bonwickii(F-Muell) Tharyley and Tetradium Sambucinum (Bl) Hartley
title_full_unstemmed Isolation and Biological Activity of Naturally Occurring Compounds from Melicope Lunu Ankenda (Gaertn) T-Hartley, Melicope Bonwickii(F-Muell) Tharyley and Tetradium Sambucinum (Bl) Hartley
title_sort isolation and biological activity of naturally occurring compounds from melicope lunu ankenda (gaertn) t-hartley, melicope bonwickii(f-muell) tharyley and tetradium sambucinum (bl) hartley
granting_institution Universiti Putra Malaysia
granting_department Science
publishDate 2005
url http://psasir.upm.edu.my/id/eprint/6248/1/FS_2005_30.pdf
_version_ 1783725712400711680

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