Generation, Phenotyping and Functional Analysis of Dendritic Cells (DC) Derived from Human Monocytes and Acute Myeloid Leukaemia (AML) Cells

Dendritic cells (DC) are efficient and potent antigen-presenting cells in our immune system. The ability of DC to present antigens and stimulate T cells has prompted their application as therapeutic cancer vaccines. The objective of this study was to generate DC from two resources: monocytes and...

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Main Author:	Lim, Moon Nian
Format:	Thesis
Language:	English English
Published:	2004
Subjects:
Online Access:	http://psasir.upm.edu.my/id/eprint/6283/1/FPSK%28M%29_2004_2.pdf
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id	my-upm-ir.6283
record_format	uketd_dc
spelling	my-upm-ir.62832023-10-20T03:29:06Z Generation, Phenotyping and Functional Analysis of Dendritic Cells (DC) Derived from Human Monocytes and Acute Myeloid Leukaemia (AML) Cells 2004-06 Lim, Moon Nian Dendritic cells (DC) are efficient and potent antigen-presenting cells in our immune system. The ability of DC to present antigens and stimulate T cells has prompted their application as therapeutic cancer vaccines. The objective of this study was to generate DC from two resources: monocytes and AML blasts. The generated DC were evaluated for their morphology by phase contrast microscopy and May Grunwald Giemsa staining. Viability of cells was determined by trypan blue dye exclusion. Percentage of yields and immunophenotypes were carried out with flow cytometry. The functional capability of DC was also tested in Mixed Leukocyte Reactions and anti-leukaemia cytotoxicity assay. As a result, the generated DC shown typical morphology of those reported and expressed DC surface markers including CDl a, CD83, CD86, CD80 and HLA-DR. Down regulation of CD14 was also observed for cultured monocytes. In MLR assay, both generated DC elicited strong allo-stimulatory response up to more than 100 fold compared to preculture cells. Mild anti-leukaemia cytotoxicity effect (1 5%) was also observed fiom primed effector cells with AML antigen pulsed DC generated fiom monocytes. These data indicate that DC were successfully generated fiom the two resources and they were capable of eliciting immune response. 2004-06 Thesis http://psasir.upm.edu.my/id/eprint/6283/ http://psasir.upm.edu.my/id/eprint/6283/1/FPSK%28M%29_2004_2.pdf text en public masters Universiti Putra Malaysia Faculty of Medicine and Health Science Seow, Heng Fong English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
advisor	Seow, Heng Fong
topic
spellingShingle	Lim, Moon Nian Generation, Phenotyping and Functional Analysis of Dendritic Cells (DC) Derived from Human Monocytes and Acute Myeloid Leukaemia (AML) Cells
description	Dendritic cells (DC) are efficient and potent antigen-presenting cells in our immune system. The ability of DC to present antigens and stimulate T cells has prompted their application as therapeutic cancer vaccines. The objective of this study was to generate DC from two resources: monocytes and AML blasts. The generated DC were evaluated for their morphology by phase contrast microscopy and May Grunwald Giemsa staining. Viability of cells was determined by trypan blue dye exclusion. Percentage of yields and immunophenotypes were carried out with flow cytometry. The functional capability of DC was also tested in Mixed Leukocyte Reactions and anti-leukaemia cytotoxicity assay. As a result, the generated DC shown typical morphology of those reported and expressed DC surface markers including CDl a, CD83, CD86, CD80 and HLA-DR. Down regulation of CD14 was also observed for cultured monocytes. In MLR assay, both generated DC elicited strong allo-stimulatory response up to more than 100 fold compared to preculture cells. Mild anti-leukaemia cytotoxicity effect (1 5%) was also observed fiom primed effector cells with AML antigen pulsed DC generated fiom monocytes. These data indicate that DC were successfully generated fiom the two resources and they were capable of eliciting immune response.
format	Thesis
qualification_level	Master's degree
author	Lim, Moon Nian
author_facet	Lim, Moon Nian
author_sort	Lim, Moon Nian
title	Generation, Phenotyping and Functional Analysis of Dendritic Cells (DC) Derived from Human Monocytes and Acute Myeloid Leukaemia (AML) Cells
title_short	Generation, Phenotyping and Functional Analysis of Dendritic Cells (DC) Derived from Human Monocytes and Acute Myeloid Leukaemia (AML) Cells
title_full	Generation, Phenotyping and Functional Analysis of Dendritic Cells (DC) Derived from Human Monocytes and Acute Myeloid Leukaemia (AML) Cells
title_fullStr	Generation, Phenotyping and Functional Analysis of Dendritic Cells (DC) Derived from Human Monocytes and Acute Myeloid Leukaemia (AML) Cells
title_full_unstemmed	Generation, Phenotyping and Functional Analysis of Dendritic Cells (DC) Derived from Human Monocytes and Acute Myeloid Leukaemia (AML) Cells
title_sort	generation, phenotyping and functional analysis of dendritic cells (dc) derived from human monocytes and acute myeloid leukaemia (aml) cells
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Medicine and Health Science
publishDate	2004
url	http://psasir.upm.edu.my/id/eprint/6283/1/FPSK%28M%29_2004_2.pdf
_version_	1783725720634130432

Generation, Phenotyping and Functional Analysis of Dendritic Cells (DC) Derived from Human Monocytes and Acute Myeloid Leukaemia (AML) Cells

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