Effects of Unfermented and Fermented Tea Ofstrobilanthes Crispus on the Proliferation of Different Cancer Cell Lines
Strobilanthes crispus ZII 109 (L) Bremek or Saricocalix crispus ZII 109 (L) Bremek (Acanthaceae) plant is originated from Indonesia. Nowadays, more variation of teas, other than Camelia sinensis tea, are produced and marketed. Since interest has now moved to other 'teas', several exper...
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Teh, Arnida Hani Effects of Unfermented and Fermented Tea Ofstrobilanthes Crispus on the Proliferation of Different Cancer Cell Lines |
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Strobilanthes crispus ZII 109 (L) Bremek or Saricocalix crispus ZII 109
(L) Bremek (Acanthaceae) plant is originated from Indonesia.
Nowadays, more variation of teas, other than Camelia sinensis tea, are
produced and marketed. Since interest has now moved to other 'teas',
several experimental tests were carried out in order to fulfill these
objectives: to develop fermented and unfermented teas of Strobilanthes
crispus leaves, to evaluate the sensory qualities, to evaluate the macro
and micro nutrient composition, to determine the total phenolics
content, and to determine the antiproliferative effects of the prepared
samples on several human cancer cell lines, i.e. liver cancer (HepG-2),
hormone-dependent breast cancer (MCF-7), non-hormone-dependent
breast cancer (MDA-MB-231), ovarian carcinoma (CAOV3) and human
cervical carcinoma cell lines (HeLa). Generally, four different products
were developed in this study; fermented Strobilanthes crispus tea (young
leaves), fermented Strobilanthes crispus tea (old leaves), unfermented
Strobilanthes crispus tea (young leaves), and unfermented Strobilanthes
crispus tea (old leaves). Overall, the moisture content of all samples do
not exceed 10%. Protein and fibre content of the samples are reported
lower than the content in the fresh leaves. However these teas provide
more carbohydrate and are high in ash levels. Calcium, sodium,
potassium, magnesium, cuprum, ferum and iron are trace element
present in all samples in various concentrations. Besides, the teas are
also good sources of antioxidant vitamins (vitamin A, C and E), which
correlate with the possible antioxidant activity. From all samples,
fermented Strobilanthes crispus tea (old leaves) showed the highest level
of vitamin A (p-carotene) value (2341.30 + 38.09 pg/g sample), while
unfermented Strobilanthes crispus tea (old leaves) is rich in vitamin C
(ascorbic acid equivalent = 5 177.88 f 1 13.96 pg/g sample) and vitamin
E (a-tocopherol equivalent = 555.91 f 77.32 pg/g sample). While
elemental analysis showed that carbon, oxygen, magnesium,
aluminum, silicon, phosphorus, sulfur, chlorine, potassium, and
calcium elements are present in all samples studied in various levels.
The results might be different from the nutritional composition values
because of the different mechanisms and methods of determination.
The total phenolics content (ferullic acid equivalent) was found highest
in unfermented Strobilanthes crispus tea (old leaves) (40.93583 + 0.70
mg/g of dried weight in methanol extract and 16.68333 f 0.53 mg/g of
dried weight in water extract). Results from the antiproliferative studies
showed that the fermented Strobilanthes crispus tea (old leaves)
methanol extract, fermented Strobilanthes crispus tea (old leaves) water
extract, unfermented Strobilanthes crispus tea (old leaves) methanol
extract and unfermented Strobilanthes crispus tea (old leaves) water
extract inhibited 50 % of the MCF-7 cell growth with ICso values = 23.0,
72.5, 63.0 and 80.5 yg/ml respectively, the unfermented Strobilanthes
crispus tea (old leaves) methanol extract, unfermented Strobilanthes
crispus tea (old leaves) water extract, fermented Strobilanthes crispus tea
(young leaves) water extract, and fermented Strobilanthes crispus tea
(old leaves) methanol extract, inhibited 50 % of the CAOV3 cell growth
with Ic50 values = 54.12, 57.22, 13.91 and 67.39 pg/ml respectively,
while unfermented Strobilanthes crispus tea (old leaves) methanol
extract and unfermented Strobilanthes crispus tea (old leaves) water
extract inhibited 50% of the HeLa cell growth with 1C50 values = 99.38
and 23.33 pg/ml respectively. On the other hand, sensory evaluation
showed that the overall acceptability score was found highest for
unfermented Strobilanthes crispus tea (old leaves), and this positively
related with its ability to inhibit 50% of the proliferation of hormonedependent
breast cancer (MCF-7), ovarian carcinoma cell lines (CAOV3)
and human cervical carcinoma cell lines (HeLa) with ICso values less
than 100 pg/ml. Generally, all the tea infusions were satisfactorily
acceptable. Nonetheless, some of the values obtained for the different
parameters for Strobilanthes crispus teas appear to fall within the range
reported for other tea leaves and, in some cases, they are higher.
Coupled with favorable amounts of minerals, fiber, vitamins, the
various phenolic compounds, and the satisfactorily acceptable taste,
colour, flavour and aroma, these teas may present a potential use as an
herbal tea worth promoting for commercialization. |
| format |
Thesis |
| qualification_level |
Master's degree |
| author |
Teh, Arnida Hani |
| author_facet |
Teh, Arnida Hani |
| author_sort |
Teh, Arnida Hani |
| title |
Effects of Unfermented and Fermented Tea Ofstrobilanthes Crispus on the Proliferation of Different Cancer Cell Lines |
| title_short |
Effects of Unfermented and Fermented Tea Ofstrobilanthes Crispus on the Proliferation of Different Cancer Cell Lines |
| title_full |
Effects of Unfermented and Fermented Tea Ofstrobilanthes Crispus on the Proliferation of Different Cancer Cell Lines |
| title_fullStr |
Effects of Unfermented and Fermented Tea Ofstrobilanthes Crispus on the Proliferation of Different Cancer Cell Lines |
| title_full_unstemmed |
Effects of Unfermented and Fermented Tea Ofstrobilanthes Crispus on the Proliferation of Different Cancer Cell Lines |
| title_sort |
effects of unfermented and fermented tea ofstrobilanthes crispus on the proliferation of different cancer cell lines |
| granting_institution |
Universiti Putra Malaysia |
| granting_department |
Faculty Medicine and Health Sciences |
| publishDate |
2006 |
| url |
http://psasir.upm.edu.my/id/eprint/6393/1/FPSK%28M%29_2006_6.pdf |
| _version_ |
1783725741947486208 |
| spelling |
my-upm-ir.63932023-10-27T01:42:03Z Effects of Unfermented and Fermented Tea Ofstrobilanthes Crispus on the Proliferation of Different Cancer Cell Lines 2006-01 Teh, Arnida Hani Strobilanthes crispus ZII 109 (L) Bremek or Saricocalix crispus ZII 109 (L) Bremek (Acanthaceae) plant is originated from Indonesia. Nowadays, more variation of teas, other than Camelia sinensis tea, are produced and marketed. Since interest has now moved to other 'teas', several experimental tests were carried out in order to fulfill these objectives: to develop fermented and unfermented teas of Strobilanthes crispus leaves, to evaluate the sensory qualities, to evaluate the macro and micro nutrient composition, to determine the total phenolics content, and to determine the antiproliferative effects of the prepared samples on several human cancer cell lines, i.e. liver cancer (HepG-2), hormone-dependent breast cancer (MCF-7), non-hormone-dependent breast cancer (MDA-MB-231), ovarian carcinoma (CAOV3) and human cervical carcinoma cell lines (HeLa). Generally, four different products were developed in this study; fermented Strobilanthes crispus tea (young leaves), fermented Strobilanthes crispus tea (old leaves), unfermented Strobilanthes crispus tea (young leaves), and unfermented Strobilanthes crispus tea (old leaves). Overall, the moisture content of all samples do not exceed 10%. Protein and fibre content of the samples are reported lower than the content in the fresh leaves. However these teas provide more carbohydrate and are high in ash levels. Calcium, sodium, potassium, magnesium, cuprum, ferum and iron are trace element present in all samples in various concentrations. Besides, the teas are also good sources of antioxidant vitamins (vitamin A, C and E), which correlate with the possible antioxidant activity. From all samples, fermented Strobilanthes crispus tea (old leaves) showed the highest level of vitamin A (p-carotene) value (2341.30 + 38.09 pg/g sample), while unfermented Strobilanthes crispus tea (old leaves) is rich in vitamin C (ascorbic acid equivalent = 5 177.88 f 1 13.96 pg/g sample) and vitamin E (a-tocopherol equivalent = 555.91 f 77.32 pg/g sample). While elemental analysis showed that carbon, oxygen, magnesium, aluminum, silicon, phosphorus, sulfur, chlorine, potassium, and calcium elements are present in all samples studied in various levels. The results might be different from the nutritional composition values because of the different mechanisms and methods of determination. The total phenolics content (ferullic acid equivalent) was found highest in unfermented Strobilanthes crispus tea (old leaves) (40.93583 + 0.70 mg/g of dried weight in methanol extract and 16.68333 f 0.53 mg/g of dried weight in water extract). Results from the antiproliferative studies showed that the fermented Strobilanthes crispus tea (old leaves) methanol extract, fermented Strobilanthes crispus tea (old leaves) water extract, unfermented Strobilanthes crispus tea (old leaves) methanol extract and unfermented Strobilanthes crispus tea (old leaves) water extract inhibited 50 % of the MCF-7 cell growth with ICso values = 23.0, 72.5, 63.0 and 80.5 yg/ml respectively, the unfermented Strobilanthes crispus tea (old leaves) methanol extract, unfermented Strobilanthes crispus tea (old leaves) water extract, fermented Strobilanthes crispus tea (young leaves) water extract, and fermented Strobilanthes crispus tea (old leaves) methanol extract, inhibited 50 % of the CAOV3 cell growth with Ic50 values = 54.12, 57.22, 13.91 and 67.39 pg/ml respectively, while unfermented Strobilanthes crispus tea (old leaves) methanol extract and unfermented Strobilanthes crispus tea (old leaves) water extract inhibited 50% of the HeLa cell growth with 1C50 values = 99.38 and 23.33 pg/ml respectively. On the other hand, sensory evaluation showed that the overall acceptability score was found highest for unfermented Strobilanthes crispus tea (old leaves), and this positively related with its ability to inhibit 50% of the proliferation of hormonedependent breast cancer (MCF-7), ovarian carcinoma cell lines (CAOV3) and human cervical carcinoma cell lines (HeLa) with ICso values less than 100 pg/ml. Generally, all the tea infusions were satisfactorily acceptable. Nonetheless, some of the values obtained for the different parameters for Strobilanthes crispus teas appear to fall within the range reported for other tea leaves and, in some cases, they are higher. Coupled with favorable amounts of minerals, fiber, vitamins, the various phenolic compounds, and the satisfactorily acceptable taste, colour, flavour and aroma, these teas may present a potential use as an herbal tea worth promoting for commercialization. 2006-01 Thesis http://psasir.upm.edu.my/id/eprint/6393/ http://psasir.upm.edu.my/id/eprint/6393/1/FPSK%28M%29_2006_6.pdf text en public masters Universiti Putra Malaysia Faculty Medicine and Health Sciences Rahmat, Asmah English |