Immunological Status of the Reproductive Tract of Ewes During Follicular and Luteal Phases

The levels of uterine Secretory-IgA (S-IgA) and numbers of plasma cells were measured to observe the differences between two stages of estrous cycle (follicular and luteal phases) in the healthy cycling non pregnant ewes. Twelve local crossbreed (Malin) ewes were used in this study and they were...

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Main Author:	Al-Medhtiy, Morteta H.
Format:	Thesis
Language:	English English
Published:	2009
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Online Access:	http://psasir.upm.edu.my/id/eprint/6555/1/abstract__FPV_2009_10.pdf
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spelling	my-upm-ir.65552013-05-27T07:30:15Z Immunological Status of the Reproductive Tract of Ewes During Follicular and Luteal Phases 2009 Al-Medhtiy, Morteta H. The levels of uterine Secretory-IgA (S-IgA) and numbers of plasma cells were measured to observe the differences between two stages of estrous cycle (follicular and luteal phases) in the healthy cycling non pregnant ewes. Twelve local crossbreed (Malin) ewes were used in this study and they were divided into two groups of 6 animals each according to the stages of estrous cycle. All ewes were subjected to estrous synchronization and were allowed to undergo one natural estrous cycle after the removal of the sponge. All animals were slaughtered at the end of the experiment. The uterine mucus was harvested by flushing with a mixture of protease inhibitor cocktail in distilled water. For both stages, the level of uterine S-IgA was quantified by using ELISA and Methyl Green Pyronine staining was used to quantify the plasma cell in the mucosa of the uterine horn and oviduct of ewe’s reproductive tract. H&E stain was used to evaluate the histological changes of endometrium during follicular and luteal phases of ewe. The protocol of the study was approved by the Faculty’s animal care and use committee (animal utilization protocol number: 08R26/Jun 08-May 09). The S-IgA level and the number of plasma cells in the reproductive tract of ewe were significantly higher (P<0.01) in the follicular phase as compared to those values in the luteal phase. 2009 Thesis http://psasir.upm.edu.my/id/eprint/6555/ http://psasir.upm.edu.my/id/eprint/6555/1/abstract__FPV_2009_10.pdf application/pdf en public masters Universiti Putra Malaysia Faculty Veterinary Medicine English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic
spellingShingle	Al-Medhtiy, Morteta H. Immunological Status of the Reproductive Tract of Ewes During Follicular and Luteal Phases
description	The levels of uterine Secretory-IgA (S-IgA) and numbers of plasma cells were measured to observe the differences between two stages of estrous cycle (follicular and luteal phases) in the healthy cycling non pregnant ewes. Twelve local crossbreed (Malin) ewes were used in this study and they were divided into two groups of 6 animals each according to the stages of estrous cycle. All ewes were subjected to estrous synchronization and were allowed to undergo one natural estrous cycle after the removal of the sponge. All animals were slaughtered at the end of the experiment. The uterine mucus was harvested by flushing with a mixture of protease inhibitor cocktail in distilled water. For both stages, the level of uterine S-IgA was quantified by using ELISA and Methyl Green Pyronine staining was used to quantify the plasma cell in the mucosa of the uterine horn and oviduct of ewe’s reproductive tract. H&E stain was used to evaluate the histological changes of endometrium during follicular and luteal phases of ewe. The protocol of the study was approved by the Faculty’s animal care and use committee (animal utilization protocol number: 08R26/Jun 08-May 09). The S-IgA level and the number of plasma cells in the reproductive tract of ewe were significantly higher (P<0.01) in the follicular phase as compared to those values in the luteal phase.
format	Thesis
qualification_level	Master's degree
author	Al-Medhtiy, Morteta H.
author_facet	Al-Medhtiy, Morteta H.
author_sort	Al-Medhtiy, Morteta H.
title	Immunological Status of the Reproductive Tract of Ewes During Follicular and Luteal Phases
title_short	Immunological Status of the Reproductive Tract of Ewes During Follicular and Luteal Phases
title_full	Immunological Status of the Reproductive Tract of Ewes During Follicular and Luteal Phases
title_fullStr	Immunological Status of the Reproductive Tract of Ewes During Follicular and Luteal Phases
title_full_unstemmed	Immunological Status of the Reproductive Tract of Ewes During Follicular and Luteal Phases
title_sort	immunological status of the reproductive tract of ewes during follicular and luteal phases
granting_institution	Universiti Putra Malaysia
granting_department	Faculty Veterinary Medicine
publishDate	2009
url	http://psasir.upm.edu.my/id/eprint/6555/1/abstract__FPV_2009_10.pdf
_version_	1747810627433267200

Immunological Status of the Reproductive Tract of Ewes During Follicular and Luteal Phases

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